Pemaparan bakteri Porphyromonas gingivalis mempengaruhi produksi superoksid netrofil The effect of Porphyromonas gingivalis induction on neutrophil’s superoxide production

• Main Authors: Nahdiya Fitriyana, Yuliana MD Arina, Happy Harmono, IDA Susilawati
• Format: Article
• Language: English
• Published: Hasanuddin University 2013-10-01
• Series: Journal of Dentomaxillofacial Science
• Subjects: free radical superoxide, P.gingivalis, neutrophil
• Online Access: http://jdmfs.org/index.php/jdmfs/article/view/370

Microbicidal mechanism with producing free radical superoxide is one of neutrophil response to the bacterial invasion. When overproducted, the free radical superoxide greater than antioxidan defense system and destroyed the cell itself. P.gingivalis had been knowed as the major bacteria that induce periodontal inflammation. These bacteria can stimulated neutrophil activities. This was aimed to determinate the superoxide production from neutrophil stimulated with P.gingivalis. Neutrophil as samples were isolated from subject who fulfill the selected criteria. The intracelluler superoxide production was determinated by nitroblue tetra zolium (NBT)test and the extracelluler production by spectrophotometer with wave length 580 nm. The result showed there was superoxide production from neutrophil stimulated by P.gingivalis. It was concluded that the stimulation of P.gingivalis on neutrophil can promote superoxide production both intracelluler and extracelluler.