In-Cell NMR: Analysis of Protein–Small Molecule Interactions, Metabolic Processes, and Protein Phosphorylation

In-Cell NMR: Analysis of Protein–Small Molecule Interactions, Metabolic Processes, and Protein Phosphorylation

Nuclear magnetic resonance (NMR) spectroscopy enables the non-invasive observation of biochemical processes, in living cells, at comparably high spectral and temporal resolution. Preferably, means of increasing the detection limit of this powerful analytical method need to be applied when observing...

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Main Authors: Amit Kumar, Lars T. Kuhn, Jochen Balbach
Format: Article
Language:English
Published: MDPI AG 2019-01-01
Series:International Journal of Molecular Sciences
Subjects:
protein NMR
in-cell NMR
in-situ NMR
DNP
review
Online Access:http://www.mdpi.com/1422-0067/20/2/378
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spelling doaj-202bddc30a4a44fea29696b539015e112020-11-24T21:25:53ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-01-0120237810.3390/ijms20020378ijms20020378In-Cell NMR: Analysis of Protein–Small Molecule Interactions, Metabolic Processes, and Protein PhosphorylationAmit Kumar0Lars T. Kuhn1Jochen Balbach2Astbury Centre for Structural Molecular Biology, School of Molecular and Cellular Biology, University of Leeds, Leeds LS2 9JT, UKAstbury Centre for Structural Molecular Biology, School of Molecular and Cellular Biology, University of Leeds, Leeds LS2 9JT, UKInstitute of Physics, Biophysics, Martin–Luther–University Halle–Wittenberg, 06120 Halle, GermanyNuclear magnetic resonance (NMR) spectroscopy enables the non-invasive observation of biochemical processes, in living cells, at comparably high spectral and temporal resolution. Preferably, means of increasing the detection limit of this powerful analytical method need to be applied when observing cellular processes under physiological conditions, due to the low sensitivity inherent to the technique. In this review, a brief introduction to in-cell NMR, protein–small molecule interactions, posttranslational phosphorylation, and hyperpolarization NMR methods, used for the study of metabolites in cellulo, are presented. Recent examples of method development in all three fields are conceptually highlighted, and an outlook into future perspectives of this emerging area of NMR research is given.http://www.mdpi.com/1422-0067/20/2/378protein NMRin-cell NMRin-situ NMRDNPreview
collection DOAJ
language English
format Article
sources DOAJ
author Amit Kumar
Lars T. Kuhn
Jochen Balbach
spellingShingle Amit Kumar
Lars T. Kuhn
Jochen Balbach
In-Cell NMR: Analysis of Protein–Small Molecule Interactions, Metabolic Processes, and Protein Phosphorylation
International Journal of Molecular Sciences
protein NMR
in-cell NMR
in-situ NMR
DNP
review
author_facet Amit Kumar
Lars T. Kuhn
Jochen Balbach
author_sort Amit Kumar
title In-Cell NMR: Analysis of Protein–Small Molecule Interactions, Metabolic Processes, and Protein Phosphorylation
title_short In-Cell NMR: Analysis of Protein–Small Molecule Interactions, Metabolic Processes, and Protein Phosphorylation
title_full In-Cell NMR: Analysis of Protein–Small Molecule Interactions, Metabolic Processes, and Protein Phosphorylation
title_fullStr In-Cell NMR: Analysis of Protein–Small Molecule Interactions, Metabolic Processes, and Protein Phosphorylation
title_full_unstemmed In-Cell NMR: Analysis of Protein–Small Molecule Interactions, Metabolic Processes, and Protein Phosphorylation
title_sort in-cell nmr: analysis of protein–small molecule interactions, metabolic processes, and protein phosphorylation
publisher MDPI AG
series International Journal of Molecular Sciences
issn 1422-0067
publishDate 2019-01-01
description Nuclear magnetic resonance (NMR) spectroscopy enables the non-invasive observation of biochemical processes, in living cells, at comparably high spectral and temporal resolution. Preferably, means of increasing the detection limit of this powerful analytical method need to be applied when observing cellular processes under physiological conditions, due to the low sensitivity inherent to the technique. In this review, a brief introduction to in-cell NMR, protein–small molecule interactions, posttranslational phosphorylation, and hyperpolarization NMR methods, used for the study of metabolites in cellulo, are presented. Recent examples of method development in all three fields are conceptually highlighted, and an outlook into future perspectives of this emerging area of NMR research is given.
topic protein NMR
in-cell NMR
in-situ NMR
DNP
review
url http://www.mdpi.com/1422-0067/20/2/378
work_keys_str_mv AT amitkumar incellnmranalysisofproteinsmallmoleculeinteractionsmetabolicprocessesandproteinphosphorylation
AT larstkuhn incellnmranalysisofproteinsmallmoleculeinteractionsmetabolicprocessesandproteinphosphorylation
AT jochenbalbach incellnmranalysisofproteinsmallmoleculeinteractionsmetabolicprocessesandproteinphosphorylation
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