Directed evolution of P-glycoprotein cysteines reveals site-specific, non-conservative substitutions that preserve multidrug resistance

Directed evolution of P-glycoprotein cysteines reveals site-specific, non-conservative substitutions that preserve multidrug resistance

Pgp (P-glycoprotein) is a prototype ABC (ATP-binding-cassette) transporter involved in multidrug resistance of cancer. We used directed evolution to replace six cytoplasmic Cys (cysteine) residues in Pgp with all 20 standard amino acids and selected for active mutants. From a pool of 75000 transfor...

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Bibliographic Details
Main Authors: Douglas J. Swartz, Leo Mok, Sri K. Botta, Anukriti Singh, Guillermo A. Altenberg, Ina L. Urbatsch
Format: Article
Language:English
Published: Portland Press, Biochemical Society 2014-06-01
Series:Bioscience Reports
Subjects:
ABC transporter
multidrug transporter
non-conservative cysteine substitutions
protein evolution site-saturation mutagenesis
yeast drug resistance
Online Access:http://www.bioscirep.org/bsr/034/e116/bsr034e116.htm

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