Directed evolution of P-glycoprotein cysteines reveals site-specific, non-conservative substitutions that preserve multidrug resistance
Pgp (P-glycoprotein) is a prototype ABC (ATP-binding-cassette) transporter involved in multidrug resistance of cancer. We used directed evolution to replace six cytoplasmic Cys (cysteine) residues in Pgp with all 20 standard amino acids and selected for active mutants. From a pool of 75000 transfor...
Main Authors: | Douglas J. Swartz, Leo Mok, Sri K. Botta, Anukriti Singh, Guillermo A. Altenberg, Ina L. Urbatsch |
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Format: | Article |
Language: | English |
Published: |
Portland Press, Biochemical Society
2014-06-01
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Series: | Bioscience Reports |
Subjects: | |
Online Access: | http://www.bioscirep.org/bsr/034/e116/bsr034e116.htm |
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