Enhanced detection of gametocytes by magnetic deposition microscopy predicts higher potential for <it>Plasmodium falciparum </it>transmission
<p>Abstract</p> <p>Background</p> <p>Aggregated haemozoin crystals within malaria-infected erythrocytes confer susceptibility of parasitized cells to a magnetic field. Here the utility of this method for diagnosis of human malaria is evaluated in a malaria-endemic regio...
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doaj-2086b6ab2fea4b0b9d3a441551b49e192020-11-25T02:18:28ZengBMCMalaria Journal1475-28752008-04-01716610.1186/1475-2875-7-66Enhanced detection of gametocytes by magnetic deposition microscopy predicts higher potential for <it>Plasmodium falciparum </it>transmissionZborowski MaciejMueller IvoMichon PascalGrimberg Brian TMoore LeeDavid MakindiKarl StephanZimmerman Peter A<p>Abstract</p> <p>Background</p> <p>Aggregated haemozoin crystals within malaria-infected erythrocytes confer susceptibility of parasitized cells to a magnetic field. Here the utility of this method for diagnosis of human malaria is evaluated in a malaria-endemic region of Papua New Guinea (PNG).</p> <p>Methods and findings</p> <p>Individuals with <it>Plasmodium falciparum </it>malaria symptoms (n = 55) provided samples for conventional blood smear (CBS) and magnetic deposition microscopy (MDM) diagnosis. Standard Giemsa staining and light microscopy was performed to evaluate all preparations. <it>Plasmodium falciparum </it>parasitaemia observed on MDM slides was consistently higher than parasitaemia observed by (CBS) for ring (CBS = 2.6 vs. MDM = 3.4%; t-test P-value = 0.13), trophozoite (CBS = 0.5 vs. MDM = 1.6%; t-test P-value = 0.01), schizont (CBS = 0.003 vs. MDM = 0.1%; t-test P-value = 0.08) and gametocyte (CBS = 0.001 vs. MDM = 0.4%; t-test P-value = 0.0002) parasitaemias. Gametocyte prevalence determined by CBS compared to MDM increased from 7.3% to 45%, respectively.</p> <p>Conclusion</p> <p>MDM increased detection sensitivity of <it>P. falciparum</it>-infected, haemozoin-containing erythrocytes from infected humans while maintaining detection of ring-stage parasites. Gametocyte prevalence five-fold higher than observed by CBS suggests higher malaria transmission potential in PNG endemic sites compared to previous estimates.</p> http://www.malariajournal.com/content/7/1/66 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zborowski Maciej Mueller Ivo Michon Pascal Grimberg Brian T Moore Lee David Makindi Karl Stephan Zimmerman Peter A |
spellingShingle |
Zborowski Maciej Mueller Ivo Michon Pascal Grimberg Brian T Moore Lee David Makindi Karl Stephan Zimmerman Peter A Enhanced detection of gametocytes by magnetic deposition microscopy predicts higher potential for <it>Plasmodium falciparum </it>transmission Malaria Journal |
author_facet |
Zborowski Maciej Mueller Ivo Michon Pascal Grimberg Brian T Moore Lee David Makindi Karl Stephan Zimmerman Peter A |
author_sort |
Zborowski Maciej |
title |
Enhanced detection of gametocytes by magnetic deposition microscopy predicts higher potential for <it>Plasmodium falciparum </it>transmission |
title_short |
Enhanced detection of gametocytes by magnetic deposition microscopy predicts higher potential for <it>Plasmodium falciparum </it>transmission |
title_full |
Enhanced detection of gametocytes by magnetic deposition microscopy predicts higher potential for <it>Plasmodium falciparum </it>transmission |
title_fullStr |
Enhanced detection of gametocytes by magnetic deposition microscopy predicts higher potential for <it>Plasmodium falciparum </it>transmission |
title_full_unstemmed |
Enhanced detection of gametocytes by magnetic deposition microscopy predicts higher potential for <it>Plasmodium falciparum </it>transmission |
title_sort |
enhanced detection of gametocytes by magnetic deposition microscopy predicts higher potential for <it>plasmodium falciparum </it>transmission |
publisher |
BMC |
series |
Malaria Journal |
issn |
1475-2875 |
publishDate |
2008-04-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Aggregated haemozoin crystals within malaria-infected erythrocytes confer susceptibility of parasitized cells to a magnetic field. Here the utility of this method for diagnosis of human malaria is evaluated in a malaria-endemic region of Papua New Guinea (PNG).</p> <p>Methods and findings</p> <p>Individuals with <it>Plasmodium falciparum </it>malaria symptoms (n = 55) provided samples for conventional blood smear (CBS) and magnetic deposition microscopy (MDM) diagnosis. Standard Giemsa staining and light microscopy was performed to evaluate all preparations. <it>Plasmodium falciparum </it>parasitaemia observed on MDM slides was consistently higher than parasitaemia observed by (CBS) for ring (CBS = 2.6 vs. MDM = 3.4%; t-test P-value = 0.13), trophozoite (CBS = 0.5 vs. MDM = 1.6%; t-test P-value = 0.01), schizont (CBS = 0.003 vs. MDM = 0.1%; t-test P-value = 0.08) and gametocyte (CBS = 0.001 vs. MDM = 0.4%; t-test P-value = 0.0002) parasitaemias. Gametocyte prevalence determined by CBS compared to MDM increased from 7.3% to 45%, respectively.</p> <p>Conclusion</p> <p>MDM increased detection sensitivity of <it>P. falciparum</it>-infected, haemozoin-containing erythrocytes from infected humans while maintaining detection of ring-stage parasites. Gametocyte prevalence five-fold higher than observed by CBS suggests higher malaria transmission potential in PNG endemic sites compared to previous estimates.</p> |
url |
http://www.malariajournal.com/content/7/1/66 |
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