Identification and assessment of Salmonella typhimurium, infantis and enteritidis serotypes in clinical samples from medical centers of Kerman province

• Main Authors: Abolfazl Moghadam, Shahram Nazarian, Jafar Amani
• Format: Article
• Language: English
• Published: Farname 2017-06-01
• Series: Iranian Journal of Medical Microbiology
• Subjects: Housekeeping genes; PCR; Salmonella typhimurium; Salmonella infantis; Salmonella enteritidis
• Online Access: http://ijmm.ir/article-1-620-en.html
• ISSN: 1735-8612; 2345-4342

Background and Aim: Salmonellosis is one of the important infectious diseases and can be as spread disease between humans and animals that make it essential for identification and detection of Salmonella. Housekeeping genes are typically important genes which are necessary for maintenance and survival of basic cells and can be considered as a gene diagnostic screening bacterial agents. The aim of this study was analysis of Flic, Sdf1 and FljB, housekeeping genes for screening of typhimurium, infantis and enteritidis serovars isolated in Kerman’s hospitals. Materials and Methods: In a descriptive study from February 2015 to August 2015, 132Salmonella specimens were taken from patients with acute gastroenteritis referred to different medical centers and hospitals in Kerman. The specimens were transferred to microbiology laboratory for identification of Salmonella with serological and bacteriological standard methods. DNA of Salmonella genus strains were extracted by CTAB and, specific primers of housekeeping genes of Salmonella genus (invA) and typhimurium(fliC), infantis(fljB) and, enteritidis(sdfI) serotypes are used in PCR test. Results: Using PCR technique, the presence of Salmonella genus were confirmed by amplification of invA gene in 130 out of 132 specimens which are identified as a Salmonella by microbiological and biochemical methods (98%). Also results indicating the prevalence of 19% in infantis, 22% in Salmonella typhimurium and 32% in Salmonella enteritidis. Conclusions: Results showed that the prevalence of Salmonella enteritidis is more than any other serotypes in this region, but as the global statistics, the prevalence of typhimurium and Infantis are increasing.