<it>Troponin T </it>isoform expression is modulated during Atlantic Halibut metamorphosis
<p>Abstract</p> <p>Background</p> <p>Flatfish metamorphosis is a thyroid hormone (TH) driven process which leads to a dramatic change from a symmetrical larva to an asymmetrical juvenile. The effect of THs on muscle and in particular muscle sarcomer protein genes is lar...
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doaj-209a00659c9346199d1bc890ce5ce9462020-11-25T01:57:22ZengBMCBMC Developmental Biology1471-213X2007-06-01717110.1186/1471-213X-7-71<it>Troponin T </it>isoform expression is modulated during Atlantic Halibut metamorphosisLlewellyn LyndaNowell Mari ASilva NádiaCampinho Marco ASweeney Glen EPower Deborah M<p>Abstract</p> <p>Background</p> <p>Flatfish metamorphosis is a thyroid hormone (TH) driven process which leads to a dramatic change from a symmetrical larva to an asymmetrical juvenile. The effect of THs on muscle and in particular muscle sarcomer protein genes is largely unexplored in fish. The change in <it>Troponin T </it>(<it>TnT</it>), a pivotal protein in the assembly of skeletal muscles sarcomeres and a modulator of calcium driven muscle contraction, during flatfish metamophosis is studied.</p> <p>Results</p> <p>In the present study five cDNAs for halibut <it>TnT </it>genes were cloned; three were splice variants arising from a single <it>fast TnT </it>(<it>fTnT</it>) gene; a fourth encoded a novel teleost specific fTnT-like cDNA (<it>AfTnT</it>) expressed exclusively in slow muscle and the fifth encoded the teleost specific <it>sTnT2</it>. THs modified the expression of halibut <it>fTnT </it>isoforms which changed from predominantly basic to acidic isoforms during natural and T4 induced metamorphosis. In contrast, expression of red muscle specific genes, <it>AfTnT </it>and <it>sTnT2</it>, did not change during natural metamorphosis or after T4 treatment. Prior to and after metamorphosis no change in the dorso-ventral symmetry or temporal-spatial expression pattern of <it>TnT </it>genes and muscle fibre organization occurred in halibut musculature.</p> <p>Conclusion</p> <p>Muscle organisation in halibut remains symmetrical even after metamorphosis suggesting TH driven changes are associated with molecular adaptations. We hypothesize that species specific differences in <it>TnT </it>gene expression in teleosts underlies different larval muscle developmental programs which better adapts them to the specific ecological constraints.</p> http://www.biomedcentral.com/1471-213X/7/71 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Llewellyn Lynda Nowell Mari A Silva Nádia Campinho Marco A Sweeney Glen E Power Deborah M |
spellingShingle |
Llewellyn Lynda Nowell Mari A Silva Nádia Campinho Marco A Sweeney Glen E Power Deborah M <it>Troponin T </it>isoform expression is modulated during Atlantic Halibut metamorphosis BMC Developmental Biology |
author_facet |
Llewellyn Lynda Nowell Mari A Silva Nádia Campinho Marco A Sweeney Glen E Power Deborah M |
author_sort |
Llewellyn Lynda |
title |
<it>Troponin T </it>isoform expression is modulated during Atlantic Halibut metamorphosis |
title_short |
<it>Troponin T </it>isoform expression is modulated during Atlantic Halibut metamorphosis |
title_full |
<it>Troponin T </it>isoform expression is modulated during Atlantic Halibut metamorphosis |
title_fullStr |
<it>Troponin T </it>isoform expression is modulated during Atlantic Halibut metamorphosis |
title_full_unstemmed |
<it>Troponin T </it>isoform expression is modulated during Atlantic Halibut metamorphosis |
title_sort |
<it>troponin t </it>isoform expression is modulated during atlantic halibut metamorphosis |
publisher |
BMC |
series |
BMC Developmental Biology |
issn |
1471-213X |
publishDate |
2007-06-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Flatfish metamorphosis is a thyroid hormone (TH) driven process which leads to a dramatic change from a symmetrical larva to an asymmetrical juvenile. The effect of THs on muscle and in particular muscle sarcomer protein genes is largely unexplored in fish. The change in <it>Troponin T </it>(<it>TnT</it>), a pivotal protein in the assembly of skeletal muscles sarcomeres and a modulator of calcium driven muscle contraction, during flatfish metamophosis is studied.</p> <p>Results</p> <p>In the present study five cDNAs for halibut <it>TnT </it>genes were cloned; three were splice variants arising from a single <it>fast TnT </it>(<it>fTnT</it>) gene; a fourth encoded a novel teleost specific fTnT-like cDNA (<it>AfTnT</it>) expressed exclusively in slow muscle and the fifth encoded the teleost specific <it>sTnT2</it>. THs modified the expression of halibut <it>fTnT </it>isoforms which changed from predominantly basic to acidic isoforms during natural and T4 induced metamorphosis. In contrast, expression of red muscle specific genes, <it>AfTnT </it>and <it>sTnT2</it>, did not change during natural metamorphosis or after T4 treatment. Prior to and after metamorphosis no change in the dorso-ventral symmetry or temporal-spatial expression pattern of <it>TnT </it>genes and muscle fibre organization occurred in halibut musculature.</p> <p>Conclusion</p> <p>Muscle organisation in halibut remains symmetrical even after metamorphosis suggesting TH driven changes are associated with molecular adaptations. We hypothesize that species specific differences in <it>TnT </it>gene expression in teleosts underlies different larval muscle developmental programs which better adapts them to the specific ecological constraints.</p> |
url |
http://www.biomedcentral.com/1471-213X/7/71 |
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