ICAM-1-carrying targeted nano contrast agent for evaluating inflammatory injury in rabbits with atherosclerosis
Abstract To investigate the feasibility of using ICAM-1-targeted nano ultrasonic contrast to evaluate the degree of inflammatory injury at different stages in the abdominal aorta of rabbits with atherosclerosis (AS). Twenty-five experimental rabbits were assigned to five groups: the control group (A...
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2021-08-01
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Online Access: | https://doi.org/10.1038/s41598-021-96042-y |
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doaj-209c9542dc404c66abe45c56eb110e852021-08-15T11:24:39ZengNature Publishing GroupScientific Reports2045-23222021-08-0111111010.1038/s41598-021-96042-yICAM-1-carrying targeted nano contrast agent for evaluating inflammatory injury in rabbits with atherosclerosisPing Li0Lin Jin1Lan Feng2Yingchun Wang3Rong Yang4Department of Ultrasound, Jiading District Central Hospital Affiliated Shanghai University of Medicine and Health SciencesDepartment of Ultrasound, Jiading District Central Hospital Affiliated Shanghai University of Medicine and Health SciencesDepartment of Ultrasound, Jiading District Central Hospital Affiliated Shanghai University of Medicine and Health SciencesDepartment of Ultrasound, Jiading District Central Hospital Affiliated Shanghai University of Medicine and Health SciencesDepartment of Pathology, Jiading District Central Hospital Affiliated Shanghai University of Medicine and Health SciencesAbstract To investigate the feasibility of using ICAM-1-targeted nano ultrasonic contrast to evaluate the degree of inflammatory injury at different stages in the abdominal aorta of rabbits with atherosclerosis (AS). Twenty-five experimental rabbits were assigned to five groups: the control group (A); the week-4 after modeling group (B); the week-8 after modeling group (C); the week-12 after modeling group (D); the week-16 after modeling group (E). All groups were given 2D ultrasonography, conventional ultrasonic contrast (SonoVue), and ICAM-1-targeted nano ultrasonic contrast, respectively. Signal intensity of vascular perfusion was evaluated. Signal intensity of ICAM-1-targeted nano ultrasonic contrast was substantially enhanced and prolonged in the vascular wall of the abdominal bubble aorta increased in B, C, D, and E groups (all P < 0.05). A positive linear correlation between intensity and the expression of ICAM-1 (r = 0.895, P < 0.001). The intensity of outer membrane was enhanced from week 4 to week 12, and both the intima-media membrane and outer membrane were enhanced with double-layer parallel echo at week 16, which was in line with the progression of atherosclerotic plaque inflammatory injury. ICAM-1-targeted nano contrast agent would be possibly a novel non-invasive molecular imaging method for plaque inflammatory injury and site high expression of specific adhesion molecules in early atherosclerotic lesions.https://doi.org/10.1038/s41598-021-96042-y |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ping Li Lin Jin Lan Feng Yingchun Wang Rong Yang |
spellingShingle |
Ping Li Lin Jin Lan Feng Yingchun Wang Rong Yang ICAM-1-carrying targeted nano contrast agent for evaluating inflammatory injury in rabbits with atherosclerosis Scientific Reports |
author_facet |
Ping Li Lin Jin Lan Feng Yingchun Wang Rong Yang |
author_sort |
Ping Li |
title |
ICAM-1-carrying targeted nano contrast agent for evaluating inflammatory injury in rabbits with atherosclerosis |
title_short |
ICAM-1-carrying targeted nano contrast agent for evaluating inflammatory injury in rabbits with atherosclerosis |
title_full |
ICAM-1-carrying targeted nano contrast agent for evaluating inflammatory injury in rabbits with atherosclerosis |
title_fullStr |
ICAM-1-carrying targeted nano contrast agent for evaluating inflammatory injury in rabbits with atherosclerosis |
title_full_unstemmed |
ICAM-1-carrying targeted nano contrast agent for evaluating inflammatory injury in rabbits with atherosclerosis |
title_sort |
icam-1-carrying targeted nano contrast agent for evaluating inflammatory injury in rabbits with atherosclerosis |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-08-01 |
description |
Abstract To investigate the feasibility of using ICAM-1-targeted nano ultrasonic contrast to evaluate the degree of inflammatory injury at different stages in the abdominal aorta of rabbits with atherosclerosis (AS). Twenty-five experimental rabbits were assigned to five groups: the control group (A); the week-4 after modeling group (B); the week-8 after modeling group (C); the week-12 after modeling group (D); the week-16 after modeling group (E). All groups were given 2D ultrasonography, conventional ultrasonic contrast (SonoVue), and ICAM-1-targeted nano ultrasonic contrast, respectively. Signal intensity of vascular perfusion was evaluated. Signal intensity of ICAM-1-targeted nano ultrasonic contrast was substantially enhanced and prolonged in the vascular wall of the abdominal bubble aorta increased in B, C, D, and E groups (all P < 0.05). A positive linear correlation between intensity and the expression of ICAM-1 (r = 0.895, P < 0.001). The intensity of outer membrane was enhanced from week 4 to week 12, and both the intima-media membrane and outer membrane were enhanced with double-layer parallel echo at week 16, which was in line with the progression of atherosclerotic plaque inflammatory injury. ICAM-1-targeted nano contrast agent would be possibly a novel non-invasive molecular imaging method for plaque inflammatory injury and site high expression of specific adhesion molecules in early atherosclerotic lesions. |
url |
https://doi.org/10.1038/s41598-021-96042-y |
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