In vivo image analysis of BoHV-4-based vector in mice.
Due to its biological characteristics bovine herpesvirus 4 (BoHV-4) has been considered as an appropriate gene delivery vector. Its genomic clone, modified as a bacterial artificial chromosome (BAC), is better genetically manipulable and can be used as an efficient gene delivery and vaccine vector....
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doaj-20a8618f001441ee84df061749c1511a2020-11-24T21:50:26ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0194e9577910.1371/journal.pone.0095779In vivo image analysis of BoHV-4-based vector in mice.Valentina FranceschiFabio Franco StellariCarlo MangiaSarah JaccaSophia LavrentiadouSandro CaviraniMathias HeikenwalderGaetano DonofrioDue to its biological characteristics bovine herpesvirus 4 (BoHV-4) has been considered as an appropriate gene delivery vector. Its genomic clone, modified as a bacterial artificial chromosome (BAC), is better genetically manipulable and can be used as an efficient gene delivery and vaccine vector. Although a large amount of data have been accumulated in vitro on this specific aspect, the same cannot be asserted for the in vivo condition. Therefore, here we investigated the fate of a recombinant BoHV-4 strain expressing luciferase (BoHV-4-A-CMVlucΔTK) after intraperitoneal or intravenous inoculation in mice, by generating a novel recombinant BoHV-4 expressing luciferase (BoHV-4-A-CMVlucΔTK) and by following the virus replication through in vivo imaging analysis. BoHV-4-A-CMVlucΔTK was first characterized in vitro where it was shown, on one hand that its replication properties are identical to those of the parental virus, and on the other that the transduced/infected cells strongly express luciferase. When BoHV-4-A-CMVlucΔTK was inoculated in mice, either intraperitoneally or intravenously, BoHV-4-A-CMVlucΔTK infection/transduction was exclusively localized to the liver, as detected by in vivo image analysis, and in particular almost exclusively in the hepatocytes, as determined by immuno-histochemistry. These data, that add a new insight on the biology of BoHV-4 in vivo, provide the first indication for the potential use of a BoHV-4-based vector in gene-transfer in the liver.http://europepmc.org/articles/PMC3994135?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Valentina Franceschi Fabio Franco Stellari Carlo Mangia Sarah Jacca Sophia Lavrentiadou Sandro Cavirani Mathias Heikenwalder Gaetano Donofrio |
spellingShingle |
Valentina Franceschi Fabio Franco Stellari Carlo Mangia Sarah Jacca Sophia Lavrentiadou Sandro Cavirani Mathias Heikenwalder Gaetano Donofrio In vivo image analysis of BoHV-4-based vector in mice. PLoS ONE |
author_facet |
Valentina Franceschi Fabio Franco Stellari Carlo Mangia Sarah Jacca Sophia Lavrentiadou Sandro Cavirani Mathias Heikenwalder Gaetano Donofrio |
author_sort |
Valentina Franceschi |
title |
In vivo image analysis of BoHV-4-based vector in mice. |
title_short |
In vivo image analysis of BoHV-4-based vector in mice. |
title_full |
In vivo image analysis of BoHV-4-based vector in mice. |
title_fullStr |
In vivo image analysis of BoHV-4-based vector in mice. |
title_full_unstemmed |
In vivo image analysis of BoHV-4-based vector in mice. |
title_sort |
in vivo image analysis of bohv-4-based vector in mice. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
Due to its biological characteristics bovine herpesvirus 4 (BoHV-4) has been considered as an appropriate gene delivery vector. Its genomic clone, modified as a bacterial artificial chromosome (BAC), is better genetically manipulable and can be used as an efficient gene delivery and vaccine vector. Although a large amount of data have been accumulated in vitro on this specific aspect, the same cannot be asserted for the in vivo condition. Therefore, here we investigated the fate of a recombinant BoHV-4 strain expressing luciferase (BoHV-4-A-CMVlucΔTK) after intraperitoneal or intravenous inoculation in mice, by generating a novel recombinant BoHV-4 expressing luciferase (BoHV-4-A-CMVlucΔTK) and by following the virus replication through in vivo imaging analysis. BoHV-4-A-CMVlucΔTK was first characterized in vitro where it was shown, on one hand that its replication properties are identical to those of the parental virus, and on the other that the transduced/infected cells strongly express luciferase. When BoHV-4-A-CMVlucΔTK was inoculated in mice, either intraperitoneally or intravenously, BoHV-4-A-CMVlucΔTK infection/transduction was exclusively localized to the liver, as detected by in vivo image analysis, and in particular almost exclusively in the hepatocytes, as determined by immuno-histochemistry. These data, that add a new insight on the biology of BoHV-4 in vivo, provide the first indication for the potential use of a BoHV-4-based vector in gene-transfer in the liver. |
url |
http://europepmc.org/articles/PMC3994135?pdf=render |
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