Induction of Stem Cell Like Cells from Mouse Embryonic Fibroblast by Short-Term Shear Stress and Vitamin C

Induction of Stem Cell Like Cells from Mouse Embryonic Fibroblast by Short-Term Shear Stress and Vitamin C

Induced pluripotent stem cells (iPSCs) are a good medicine source because of their potential to differentiate into various tissues or cells. However, traditionally, iPSCs made by specific transgenes and virus vectors are not appropriate for clinical use because of safety concerns and risk of tumor d...

Full description

Bibliographic Details
Main Authors: Seungmin Yeom, Myung Chul Lee, Shambhavi Pandey, Jaewoon Lim, Sangbae Park, Jae Eun Kim, Kyoung Je Jang, Hoon Seonwoo, Pankaj Garg, Jong Hoon Chung
Format: Article
Language:English
Published: MDPI AG 2021-02-01
Series:Applied Sciences
Subjects:
mouse embryonic fibroblast
reprogramming
sphere formation
shear stress
vitamin C
Online Access:https://www.mdpi.com/2076-3417/11/4/1941
id doaj-20acd97c77074f6e8f6975be90a5a25b
record_format Article
spelling doaj-20acd97c77074f6e8f6975be90a5a25b2021-02-24T00:00:52ZengMDPI AGApplied Sciences2076-34172021-02-01111941194110.3390/app11041941Induction of Stem Cell Like Cells from Mouse Embryonic Fibroblast by Short-Term Shear Stress and Vitamin CSeungmin Yeom0Myung Chul Lee1Shambhavi Pandey2Jaewoon Lim3Sangbae Park4Jae Eun Kim5Kyoung Je Jang6Hoon Seonwoo7Pankaj Garg8Jong Hoon Chung9Department of Biosystems & Biomaterial Science and Engineering, Seoul National University, Seoul 08826, KoreaDepartment of Biosystems & Biomaterial Science and Engineering, Seoul National University, Seoul 08826, KoreaResearch Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, KoreaDepartment of Biosystems & Biomaterial Science and Engineering, Seoul National University, Seoul 08826, KoreaDepartment of Biosystems & Biomaterial Science and Engineering, Seoul National University, Seoul 08826, KoreaDepartment of Biosystems Engineering, Seoul National University, Seoul 08826, KoreaDivision of Agro-system Engineering, Gyeongsang National University, Jinju 52828, KoreaDepartment of Industrial Machinery Engineering, College of Life Science and Natural Resources, Sunchon National University, Sunchon 57922, KoreaResearch Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, KoreaResearch Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, KoreaInduced pluripotent stem cells (iPSCs) are a good medicine source because of their potential to differentiate into various tissues or cells. However, traditionally, iPSCs made by specific transgenes and virus vectors are not appropriate for clinical use because of safety concerns and risk of tumor development. The goal of this research was to develop an alternative method for reprogramming, using small molecules and external stimuli. Two groups were established: short-term shear stress (STSS) under suspension culture and a combination of short-term shear stress and vitamin C (SSVC) under suspension culture. For STSS, the pipetting was carried out for cells twice per day for 2 min for 14 days in the embryonic stem cell (ES) medium. In the case of SSVC, the procedure was the same as for STSS however, its ES medium included 10 µM of vitamin C. After 14 days, all spheroids were picked and checked for pluripotency by ALP (alkaline phosphatase) assay and immunocytochemistry. Both groups partially showed the characteristics of stem cells but data demonstrated that the spheroids under shear stress and vitamin C had improved stem cell-like properties. This research showed the possibility of external stimuli and small molecules to reprogram the somatic cells without the use of transgenes.https://www.mdpi.com/2076-3417/11/4/1941mouse embryonic fibroblastreprogrammingsphere formationshear stressvitamin C
collection DOAJ
language English
format Article
sources DOAJ
author Seungmin Yeom
Myung Chul Lee
Shambhavi Pandey
Jaewoon Lim
Sangbae Park
Jae Eun Kim
Kyoung Je Jang
Hoon Seonwoo
Pankaj Garg
Jong Hoon Chung
spellingShingle Seungmin Yeom
Myung Chul Lee
Shambhavi Pandey
Jaewoon Lim
Sangbae Park
Jae Eun Kim
Kyoung Je Jang
Hoon Seonwoo
Pankaj Garg
Jong Hoon Chung
Induction of Stem Cell Like Cells from Mouse Embryonic Fibroblast by Short-Term Shear Stress and Vitamin C
Applied Sciences
mouse embryonic fibroblast
reprogramming
sphere formation
shear stress
vitamin C
author_facet Seungmin Yeom
Myung Chul Lee
Shambhavi Pandey
Jaewoon Lim
Sangbae Park
Jae Eun Kim
Kyoung Je Jang
Hoon Seonwoo
Pankaj Garg
Jong Hoon Chung
author_sort Seungmin Yeom
title Induction of Stem Cell Like Cells from Mouse Embryonic Fibroblast by Short-Term Shear Stress and Vitamin C
title_short Induction of Stem Cell Like Cells from Mouse Embryonic Fibroblast by Short-Term Shear Stress and Vitamin C
title_full Induction of Stem Cell Like Cells from Mouse Embryonic Fibroblast by Short-Term Shear Stress and Vitamin C
title_fullStr Induction of Stem Cell Like Cells from Mouse Embryonic Fibroblast by Short-Term Shear Stress and Vitamin C
title_full_unstemmed Induction of Stem Cell Like Cells from Mouse Embryonic Fibroblast by Short-Term Shear Stress and Vitamin C
title_sort induction of stem cell like cells from mouse embryonic fibroblast by short-term shear stress and vitamin c
publisher MDPI AG
series Applied Sciences
issn 2076-3417
publishDate 2021-02-01
description Induced pluripotent stem cells (iPSCs) are a good medicine source because of their potential to differentiate into various tissues or cells. However, traditionally, iPSCs made by specific transgenes and virus vectors are not appropriate for clinical use because of safety concerns and risk of tumor development. The goal of this research was to develop an alternative method for reprogramming, using small molecules and external stimuli. Two groups were established: short-term shear stress (STSS) under suspension culture and a combination of short-term shear stress and vitamin C (SSVC) under suspension culture. For STSS, the pipetting was carried out for cells twice per day for 2 min for 14 days in the embryonic stem cell (ES) medium. In the case of SSVC, the procedure was the same as for STSS however, its ES medium included 10 µM of vitamin C. After 14 days, all spheroids were picked and checked for pluripotency by ALP (alkaline phosphatase) assay and immunocytochemistry. Both groups partially showed the characteristics of stem cells but data demonstrated that the spheroids under shear stress and vitamin C had improved stem cell-like properties. This research showed the possibility of external stimuli and small molecules to reprogram the somatic cells without the use of transgenes.
topic mouse embryonic fibroblast
reprogramming
sphere formation
shear stress
vitamin C
url https://www.mdpi.com/2076-3417/11/4/1941
work_keys_str_mv AT seungminyeom inductionofstemcelllikecellsfrommouseembryonicfibroblastbyshorttermshearstressandvitaminc
AT myungchullee inductionofstemcelllikecellsfrommouseembryonicfibroblastbyshorttermshearstressandvitaminc
AT shambhavipandey inductionofstemcelllikecellsfrommouseembryonicfibroblastbyshorttermshearstressandvitaminc
AT jaewoonlim inductionofstemcelllikecellsfrommouseembryonicfibroblastbyshorttermshearstressandvitaminc
AT sangbaepark inductionofstemcelllikecellsfrommouseembryonicfibroblastbyshorttermshearstressandvitaminc
AT jaeeunkim inductionofstemcelllikecellsfrommouseembryonicfibroblastbyshorttermshearstressandvitaminc
AT kyoungjejang inductionofstemcelllikecellsfrommouseembryonicfibroblastbyshorttermshearstressandvitaminc
AT hoonseonwoo inductionofstemcelllikecellsfrommouseembryonicfibroblastbyshorttermshearstressandvitaminc
AT pankajgarg inductionofstemcelllikecellsfrommouseembryonicfibroblastbyshorttermshearstressandvitaminc
AT jonghoonchung inductionofstemcelllikecellsfrommouseembryonicfibroblastbyshorttermshearstressandvitaminc
_version_ 1724253691710537728

Similar Items