Alterations in blood glucose and plasma glucagon concentrations during deep brain stimulation in the shell region of the nucleus accumbens in rats
Deep brain stimulation (DBS) of the nucleus accumbens (NAc) is an effective therapy for obsessive compulsive disorder (OCD) and is currently under investigation as a treatment for eating disorders. DBS of this area is associated with altered food intake and pharmacological treatment of OCD is associ...
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doaj-20ad69143a194d6993ce4a24b523adaf2020-11-24T20:42:48ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2013-12-01710.3389/fnins.2013.0022663611Alterations in blood glucose and plasma glucagon concentrations during deep brain stimulation in the shell region of the nucleus accumbens in ratsCharlene eDiepenbroek0Geoffrey evan der Plasse1Geoffrey evan der Plasse2Leslie eEggels3Merel eRijnsburger4Matthijs eFeenstra5Matthijs eFeenstra6Andries eKalsbeek7Andries eKalsbeek8Damiaan eDenys9Damiaan eDenys10Eric eFliers11Mireille J Serlie12Susanne E la Fleur13Academic Medical Center, University of AmsterdamBrain Center Rudolf Magnus, University Medical CenterBrain Center Rudolf Magnus, University Medical CenterAcademic Medical Center, University of AmsterdamAcademic Medical Center, University of AmsterdamNetherlands Institute for Neuroscience, an institute of the Royal Netherlands Academy of Arts and SciencesAcademic Medical Center, University of AmsterdamAcademic Medical Center, University of AmsterdamNetherlands Institute for Neuroscience, an institute of the Royal Netherlands Academy of Arts and SciencesNetherlands Institute for Neuroscience, an institute of the Royal Netherlands Academy of Arts and SciencesAcademic Medical Center, University of AmsterdamAcademic Medical Center, University of AmsterdamAcademic Medical Center, University of AmsterdamAcademic Medical Center, University of AmsterdamDeep brain stimulation (DBS) of the nucleus accumbens (NAc) is an effective therapy for obsessive compulsive disorder (OCD) and is currently under investigation as a treatment for eating disorders. DBS of this area is associated with altered food intake and pharmacological treatment of OCD is associated with the risk of developing type 2 diabetes. Therefore we examined if DBS of the NAc-shell (sNAc) influences glucose metabolism. Male Wistar rats were subjected to DBS, or sham stimulation, for a period of one hour. To assess the effects of stimulation on blood glucose and glucoregulatory hormones, blood samples were drawn before, during and after stimulation. Subsequently, all animals were used for quantitative assessment of Fos immunoreactivity in the lateral hypothalamic area (LHA) using computerized image analysis. DBS of the sNAc rapidly increased plasma concentrations of glucagon and glucose while sham stimulation and DBS outside the sNAc were ineffective. In addition, the increase in glucose was dependent on DBS intensity. In contrast, the DBS-induced increase in plasma corticosterone concentrations was independent of intensity and region, indicating that the observed DBS-induced metabolic changes were not due to corticosterone release. Stimulation of the sNAc with 200 μA increased Fos immunoreactivity in the LHA compared to sham or 100 μA stimulated animals. These data show that DBS of the sNAc alters glucose metabolism in a region- and intensity dependent manner in association with neuronal activation in the LHA. Moreover, these data illustrate the need to monitor changes in glucose metabolism during DBS-treatment of OCD patients.http://journal.frontiersin.org/Journal/10.3389/fnins.2013.00226/fullGlucoseneural activitydeep brain stimulation (DBS)Foslateral hypothalamic areanucleus accumbens shell |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Charlene eDiepenbroek Geoffrey evan der Plasse Geoffrey evan der Plasse Leslie eEggels Merel eRijnsburger Matthijs eFeenstra Matthijs eFeenstra Andries eKalsbeek Andries eKalsbeek Damiaan eDenys Damiaan eDenys Eric eFliers Mireille J Serlie Susanne E la Fleur |
spellingShingle |
Charlene eDiepenbroek Geoffrey evan der Plasse Geoffrey evan der Plasse Leslie eEggels Merel eRijnsburger Matthijs eFeenstra Matthijs eFeenstra Andries eKalsbeek Andries eKalsbeek Damiaan eDenys Damiaan eDenys Eric eFliers Mireille J Serlie Susanne E la Fleur Alterations in blood glucose and plasma glucagon concentrations during deep brain stimulation in the shell region of the nucleus accumbens in rats Frontiers in Neuroscience Glucose neural activity deep brain stimulation (DBS) Fos lateral hypothalamic area nucleus accumbens shell |
author_facet |
Charlene eDiepenbroek Geoffrey evan der Plasse Geoffrey evan der Plasse Leslie eEggels Merel eRijnsburger Matthijs eFeenstra Matthijs eFeenstra Andries eKalsbeek Andries eKalsbeek Damiaan eDenys Damiaan eDenys Eric eFliers Mireille J Serlie Susanne E la Fleur |
author_sort |
Charlene eDiepenbroek |
title |
Alterations in blood glucose and plasma glucagon concentrations during deep brain stimulation in the shell region of the nucleus accumbens in rats |
title_short |
Alterations in blood glucose and plasma glucagon concentrations during deep brain stimulation in the shell region of the nucleus accumbens in rats |
title_full |
Alterations in blood glucose and plasma glucagon concentrations during deep brain stimulation in the shell region of the nucleus accumbens in rats |
title_fullStr |
Alterations in blood glucose and plasma glucagon concentrations during deep brain stimulation in the shell region of the nucleus accumbens in rats |
title_full_unstemmed |
Alterations in blood glucose and plasma glucagon concentrations during deep brain stimulation in the shell region of the nucleus accumbens in rats |
title_sort |
alterations in blood glucose and plasma glucagon concentrations during deep brain stimulation in the shell region of the nucleus accumbens in rats |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Neuroscience |
issn |
1662-453X |
publishDate |
2013-12-01 |
description |
Deep brain stimulation (DBS) of the nucleus accumbens (NAc) is an effective therapy for obsessive compulsive disorder (OCD) and is currently under investigation as a treatment for eating disorders. DBS of this area is associated with altered food intake and pharmacological treatment of OCD is associated with the risk of developing type 2 diabetes. Therefore we examined if DBS of the NAc-shell (sNAc) influences glucose metabolism. Male Wistar rats were subjected to DBS, or sham stimulation, for a period of one hour. To assess the effects of stimulation on blood glucose and glucoregulatory hormones, blood samples were drawn before, during and after stimulation. Subsequently, all animals were used for quantitative assessment of Fos immunoreactivity in the lateral hypothalamic area (LHA) using computerized image analysis. DBS of the sNAc rapidly increased plasma concentrations of glucagon and glucose while sham stimulation and DBS outside the sNAc were ineffective. In addition, the increase in glucose was dependent on DBS intensity. In contrast, the DBS-induced increase in plasma corticosterone concentrations was independent of intensity and region, indicating that the observed DBS-induced metabolic changes were not due to corticosterone release. Stimulation of the sNAc with 200 μA increased Fos immunoreactivity in the LHA compared to sham or 100 μA stimulated animals. These data show that DBS of the sNAc alters glucose metabolism in a region- and intensity dependent manner in association with neuronal activation in the LHA. Moreover, these data illustrate the need to monitor changes in glucose metabolism during DBS-treatment of OCD patients. |
topic |
Glucose neural activity deep brain stimulation (DBS) Fos lateral hypothalamic area nucleus accumbens shell |
url |
http://journal.frontiersin.org/Journal/10.3389/fnins.2013.00226/full |
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