Component-Metabolome Correlations of Gut Microbiota from Child-Turcotte-Pugh of A and B patients
The gut flora are widely involved in the cometabolism with the host and have evident effects on the metabolic phenotype of host. This study performed a metabolome analysis of the intestinal microbiota specific for liver cirrhosis. The study population included patients with Child-Turcotte-Pugh (CTP)...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2016-11-01
|
Series: | Frontiers in Microbiology |
Subjects: | |
Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01856/full |
id |
doaj-20dd0fbaa97d4c2d929911621716645a |
---|---|
record_format |
Article |
spelling |
doaj-20dd0fbaa97d4c2d929911621716645a2020-11-24T21:54:04ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-11-01710.3389/fmicb.2016.01856224770Component-Metabolome Correlations of Gut Microbiota from Child-Turcotte-Pugh of A and B patientsXiao Wei0Shan Jiang1Xiangna Zhao2Huan Li3Weishi Lin4Boxing Li5Jing Lu6Yansong Sun7Jing Yuan8Institute of disease control and preventionInstitute of disease control and preventionInstitute of disease control and preventionInstitute of disease control and preventionInstitute of disease control and preventionInstitute of disease control and preventionInstitute of disease control and preventionInstitute of disease control and preventionInstitute of disease control and preventionThe gut flora are widely involved in the cometabolism with the host and have evident effects on the metabolic phenotype of host. This study performed a metabolome analysis of the intestinal microbiota specific for liver cirrhosis. The study population included patients with Child-Turcotte-Pugh (CTP) score of A (AP, n=5) and B (BP, n=5), and control subjects (NM, n=3). Metagenomic DNA from fecal microbiota was extracted followed by metagenomic sequenceing through Illumina MiSeq high throughput sequencing of 16S rRNA regions. The detection of metabolites from fecal samples was performed using high-performance liquid phase chromatography and gas chromatography coupled with tandem mass spectrometry (HPLC-GC/MS-MS). Intestinal microbiota community and metabolite analysis both showed separation of cirrhotic patients from control participants, moreover, the microbiota-metabolite correlations changed in cirrhotic patients. Fecal microbiota from cirrhotic patients, with the reduced diversity, contained a decreased abundance of Bacteroidetes and an increased abundance of Proteobacteria compared with the normal samples. Analysis of metabolome revealed a remarkable change in the metabolic potential of the microbiota in cirrhotic patients, with specific higher concentrations of amine, unsaturated fatty acid, and SCFAs (short-chain fatty acids), and lower concentrations of sugar alcohol and amino acid, suggesting the initial equilibrium of gut microbiota community and co-metabolism with the host were perturbed by cirrhosis. Our study illustrated the relationship between fecal microbiota composition and metabolom in cirrhotic patients, which may improve the clinical prognosis of cirrhosis.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01856/fullLiver CirrhosisMetabolomeAmino acidGut MicrobiotaSCFAs |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiao Wei Shan Jiang Xiangna Zhao Huan Li Weishi Lin Boxing Li Jing Lu Yansong Sun Jing Yuan |
spellingShingle |
Xiao Wei Shan Jiang Xiangna Zhao Huan Li Weishi Lin Boxing Li Jing Lu Yansong Sun Jing Yuan Component-Metabolome Correlations of Gut Microbiota from Child-Turcotte-Pugh of A and B patients Frontiers in Microbiology Liver Cirrhosis Metabolome Amino acid Gut Microbiota SCFAs |
author_facet |
Xiao Wei Shan Jiang Xiangna Zhao Huan Li Weishi Lin Boxing Li Jing Lu Yansong Sun Jing Yuan |
author_sort |
Xiao Wei |
title |
Component-Metabolome Correlations of Gut Microbiota from Child-Turcotte-Pugh of A and B patients |
title_short |
Component-Metabolome Correlations of Gut Microbiota from Child-Turcotte-Pugh of A and B patients |
title_full |
Component-Metabolome Correlations of Gut Microbiota from Child-Turcotte-Pugh of A and B patients |
title_fullStr |
Component-Metabolome Correlations of Gut Microbiota from Child-Turcotte-Pugh of A and B patients |
title_full_unstemmed |
Component-Metabolome Correlations of Gut Microbiota from Child-Turcotte-Pugh of A and B patients |
title_sort |
component-metabolome correlations of gut microbiota from child-turcotte-pugh of a and b patients |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2016-11-01 |
description |
The gut flora are widely involved in the cometabolism with the host and have evident effects on the metabolic phenotype of host. This study performed a metabolome analysis of the intestinal microbiota specific for liver cirrhosis. The study population included patients with Child-Turcotte-Pugh (CTP) score of A (AP, n=5) and B (BP, n=5), and control subjects (NM, n=3). Metagenomic DNA from fecal microbiota was extracted followed by metagenomic sequenceing through Illumina MiSeq high throughput sequencing of 16S rRNA regions. The detection of metabolites from fecal samples was performed using high-performance liquid phase chromatography and gas chromatography coupled with tandem mass spectrometry (HPLC-GC/MS-MS). Intestinal microbiota community and metabolite analysis both showed separation of cirrhotic patients from control participants, moreover, the microbiota-metabolite correlations changed in cirrhotic patients. Fecal microbiota from cirrhotic patients, with the reduced diversity, contained a decreased abundance of Bacteroidetes and an increased abundance of Proteobacteria compared with the normal samples. Analysis of metabolome revealed a remarkable change in the metabolic potential of the microbiota in cirrhotic patients, with specific higher concentrations of amine, unsaturated fatty acid, and SCFAs (short-chain fatty acids), and lower concentrations of sugar alcohol and amino acid, suggesting the initial equilibrium of gut microbiota community and co-metabolism with the host were perturbed by cirrhosis. Our study illustrated the relationship between fecal microbiota composition and metabolom in cirrhotic patients, which may improve the clinical prognosis of cirrhosis. |
topic |
Liver Cirrhosis Metabolome Amino acid Gut Microbiota SCFAs |
url |
http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01856/full |
work_keys_str_mv |
AT xiaowei componentmetabolomecorrelationsofgutmicrobiotafromchildturcottepughofaandbpatients AT shanjiang componentmetabolomecorrelationsofgutmicrobiotafromchildturcottepughofaandbpatients AT xiangnazhao componentmetabolomecorrelationsofgutmicrobiotafromchildturcottepughofaandbpatients AT huanli componentmetabolomecorrelationsofgutmicrobiotafromchildturcottepughofaandbpatients AT weishilin componentmetabolomecorrelationsofgutmicrobiotafromchildturcottepughofaandbpatients AT boxingli componentmetabolomecorrelationsofgutmicrobiotafromchildturcottepughofaandbpatients AT jinglu componentmetabolomecorrelationsofgutmicrobiotafromchildturcottepughofaandbpatients AT yansongsun componentmetabolomecorrelationsofgutmicrobiotafromchildturcottepughofaandbpatients AT jingyuan componentmetabolomecorrelationsofgutmicrobiotafromchildturcottepughofaandbpatients |
_version_ |
1725869226279829504 |