Inter-Dye Distance Distributions Studied by a Combination of Single-Molecule FRET-Filtered Lifetime Measurements and a Weighted Accessible Volume (wAV) Algorithm

Inter-Dye Distance Distributions Studied by a Combination of Single-Molecule FRET-Filtered Lifetime Measurements and a Weighted Accessible Volume (wAV) Algorithm

Förster resonance energy transfer (FRET) is an important tool for studying the structural and dynamical properties of biomolecules. The fact that both the internal dynamics of the biomolecule and the movements of the biomolecule-attached dyes can occur on similar timescales of nanoseconds is an inhe...

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Bibliographic Details
Main Authors: Henning Höfig, Matteo Gabba, Simón Poblete, Daryan Kempe, Jörg Fitter
Format: Article
Language:English
Published: MDPI AG 2014-11-01
Series:Molecules
Subjects:
smFRET
lifetime FRET measurements
dye-linker dynamics
protein dynamics
Online Access:http://www.mdpi.com/1420-3049/19/12/19269

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