Immunohistochemical Differences in Squamous Precancerous and Cancerous Lesions of the Oral Cavity and the Larynx: Preliminary Data
The aim of this study is to assess immune cell populations in squamous precancerous (preinvasive) and cancerous lesions of the oral cavity and larynx. Qualitative and quantitative immunohistochemical analyses were performed to determine the expressions of CD4, CD8, CD15, CD57 and CD68. The expressio...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-02-01
|
Series: | Applied Sciences |
Subjects: | |
Online Access: | https://www.mdpi.com/2076-3417/11/5/2048 |
id |
doaj-20ef932acf534c259e80e90ca2d22233 |
---|---|
record_format |
Article |
spelling |
doaj-20ef932acf534c259e80e90ca2d222332021-02-26T00:05:52ZengMDPI AGApplied Sciences2076-34172021-02-01112048204810.3390/app11052048Immunohistochemical Differences in Squamous Precancerous and Cancerous Lesions of the Oral Cavity and the Larynx: Preliminary DataPiero Luigi Aló0Miriam Cicciarelli1Francesca De Felice2Claudia Quintiliani3Alessandro Corsi4Antonella Polimeni5Department DAF, Anatomic Pathology, ASL Frosinone, Via Armando Fabi, 03100 Frosinone, ItalyDepartment of Molecular Medicine, “Sapienza” University of Rome, Policlinico Umberto I, Viale Regina Elena 324, 00161 Rome, ItalyDepartment of of Radiological, Oncological and Pathological Sciences, Section of Radiotherapy, “Sapienza” University of Rome, Policlinico Umberto I, Viale Regina Elena 324, 00161 Rome, ItalyDepartment DAF, Anatomic Pathology, ASL Frosinone, Via Armando Fabi, 03100 Frosinone, ItalyDepartment of Molecular Medicine, “Sapienza” University of Rome, Policlinico Umberto I, Viale Regina Elena 324, 00161 Rome, ItalyDepartment of Oral and Maxillo Facial Sciences, “Sapienza” University of Rome, Policlinico Umberto I, Via Caserta 6, 00161 Rome, ItalyThe aim of this study is to assess immune cell populations in squamous precancerous (preinvasive) and cancerous lesions of the oral cavity and larynx. Qualitative and quantitative immunohistochemical analyses were performed to determine the expressions of CD4, CD8, CD15, CD57 and CD68. The expressions of programmed death-ligand 1 (PD-L1), p16 and Ki67 were also assessed. Squamous cell lesions from forty-one patients were included in the study. Sixteen samples were categorized as precancerous (preinvasive) lesions and twenty-five as invasive squamous cell carcinoma. Invasive lesions showed a negative correlation with CD57+ cells (ρ = −0.69) and a positive correlation with Ki67 (ρ = 0.61). The amount of CD4+ lymphocytes was higher in invasive lesions. There were no differences in PD-L1 and p16 immunoreactivity. Our analysis showed differences in the immunohistochemical profile between preinvasive and invasive squamous cell lesions. In the near future, this study should be useful in driving treatment strategy in both preinvasive and invasive squamous cell lesions of the oral cavity and larynx. However, studies on larger series of patients focusing on site-specific lesions are required.https://www.mdpi.com/2076-3417/11/5/2048oral cavity squamous cell carcinomalaryngeal squamous cell carcinomapreinvasive squamous cell lesionstumor associated immune cellsPD-L1Ki67 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Piero Luigi Aló Miriam Cicciarelli Francesca De Felice Claudia Quintiliani Alessandro Corsi Antonella Polimeni |
spellingShingle |
Piero Luigi Aló Miriam Cicciarelli Francesca De Felice Claudia Quintiliani Alessandro Corsi Antonella Polimeni Immunohistochemical Differences in Squamous Precancerous and Cancerous Lesions of the Oral Cavity and the Larynx: Preliminary Data Applied Sciences oral cavity squamous cell carcinoma laryngeal squamous cell carcinoma preinvasive squamous cell lesions tumor associated immune cells PD-L1 Ki67 |
author_facet |
Piero Luigi Aló Miriam Cicciarelli Francesca De Felice Claudia Quintiliani Alessandro Corsi Antonella Polimeni |
author_sort |
Piero Luigi Aló |
title |
Immunohistochemical Differences in Squamous Precancerous and Cancerous Lesions of the Oral Cavity and the Larynx: Preliminary Data |
title_short |
Immunohistochemical Differences in Squamous Precancerous and Cancerous Lesions of the Oral Cavity and the Larynx: Preliminary Data |
title_full |
Immunohistochemical Differences in Squamous Precancerous and Cancerous Lesions of the Oral Cavity and the Larynx: Preliminary Data |
title_fullStr |
Immunohistochemical Differences in Squamous Precancerous and Cancerous Lesions of the Oral Cavity and the Larynx: Preliminary Data |
title_full_unstemmed |
Immunohistochemical Differences in Squamous Precancerous and Cancerous Lesions of the Oral Cavity and the Larynx: Preliminary Data |
title_sort |
immunohistochemical differences in squamous precancerous and cancerous lesions of the oral cavity and the larynx: preliminary data |
publisher |
MDPI AG |
series |
Applied Sciences |
issn |
2076-3417 |
publishDate |
2021-02-01 |
description |
The aim of this study is to assess immune cell populations in squamous precancerous (preinvasive) and cancerous lesions of the oral cavity and larynx. Qualitative and quantitative immunohistochemical analyses were performed to determine the expressions of CD4, CD8, CD15, CD57 and CD68. The expressions of programmed death-ligand 1 (PD-L1), p16 and Ki67 were also assessed. Squamous cell lesions from forty-one patients were included in the study. Sixteen samples were categorized as precancerous (preinvasive) lesions and twenty-five as invasive squamous cell carcinoma. Invasive lesions showed a negative correlation with CD57+ cells (ρ = −0.69) and a positive correlation with Ki67 (ρ = 0.61). The amount of CD4+ lymphocytes was higher in invasive lesions. There were no differences in PD-L1 and p16 immunoreactivity. Our analysis showed differences in the immunohistochemical profile between preinvasive and invasive squamous cell lesions. In the near future, this study should be useful in driving treatment strategy in both preinvasive and invasive squamous cell lesions of the oral cavity and larynx. However, studies on larger series of patients focusing on site-specific lesions are required. |
topic |
oral cavity squamous cell carcinoma laryngeal squamous cell carcinoma preinvasive squamous cell lesions tumor associated immune cells PD-L1 Ki67 |
url |
https://www.mdpi.com/2076-3417/11/5/2048 |
work_keys_str_mv |
AT pieroluigialo immunohistochemicaldifferencesinsquamousprecancerousandcancerouslesionsoftheoralcavityandthelarynxpreliminarydata AT miriamcicciarelli immunohistochemicaldifferencesinsquamousprecancerousandcancerouslesionsoftheoralcavityandthelarynxpreliminarydata AT francescadefelice immunohistochemicaldifferencesinsquamousprecancerousandcancerouslesionsoftheoralcavityandthelarynxpreliminarydata AT claudiaquintiliani immunohistochemicaldifferencesinsquamousprecancerousandcancerouslesionsoftheoralcavityandthelarynxpreliminarydata AT alessandrocorsi immunohistochemicaldifferencesinsquamousprecancerousandcancerouslesionsoftheoralcavityandthelarynxpreliminarydata AT antonellapolimeni immunohistochemicaldifferencesinsquamousprecancerousandcancerouslesionsoftheoralcavityandthelarynxpreliminarydata |
_version_ |
1724250196197507072 |