Anomalous Dense Liquid Condensates Host the Nucleation of Tumor Suppressor p53 Fibrils
Summary: About half of human cancers are associated with mutations of the tumor suppressor p53. Gained oncogenic functions of the mutants have been related to aggregation behaviors of wild-type and mutant p53. The thermodynamic and kinetic mechanisms of p53 aggregation are poorly understood. Here we...
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doaj-2115390650724f12a24b2194be1d87df2020-11-25T00:44:25ZengElsevieriScience2589-00422019-02-0112342355Anomalous Dense Liquid Condensates Host the Nucleation of Tumor Suppressor p53 FibrilsMohammad S. Safari0Zhiqing Wang1Kunaal Tailor2Anatoly B. Kolomeisky3Jacinta C. Conrad4Peter G. Vekilov5Department of Chemical and Biomolecular Engineering, University of Houston, 4726 Calhoun Road, Houston, TX 77204-4004, USA; Department of Molecular Biology, Princeton University, Princeton, NJ 08544-1014, USADepartment of Chemical and Biomolecular Engineering, University of Houston, 4726 Calhoun Road, Houston, TX 77204-4004, USADepartment of Chemical and Biomolecular Engineering, University of Houston, 4726 Calhoun Road, Houston, TX 77204-4004, USADepartment of Chemistry, Rice University, P.O. Box 1892, MS 60, Houston, TX 77251-1892, USA; Corresponding authorDepartment of Chemical and Biomolecular Engineering, University of Houston, 4726 Calhoun Road, Houston, TX 77204-4004, USA; Corresponding authorDepartment of Chemical and Biomolecular Engineering, University of Houston, 4726 Calhoun Road, Houston, TX 77204-4004, USA; Department of Chemistry, University of Houston, 3585 Cullen Boulevard, Houston, TX 77204-5003, USA; Corresponding authorSummary: About half of human cancers are associated with mutations of the tumor suppressor p53. Gained oncogenic functions of the mutants have been related to aggregation behaviors of wild-type and mutant p53. The thermodynamic and kinetic mechanisms of p53 aggregation are poorly understood. Here we find that wild-type p53 forms an anomalous liquid phase. The liquid condensates exhibit several behaviors beyond the scope of classical phase transition theories: their size, ca. 100 nm, is independent of the p53 concentration and decoupled from the protein mass held in the liquid phase. Furthermore, the liquid phase lacks constant solubility. The nucleation of p53 fibrils deviates from the accepted mechanism of sequential association of single solute molecules. We find that the liquid condensates serve as pre-assembled precursors of high p53 concentration that facilitate fibril assembly. Fibril nucleation hosted by precursors represents a novel biological pathway, which opens avenues to suppress protein fibrillation in aggregation diseases. : Chemistry; Biological Sciences; Biophysics Subject Areas: Chemistry, Biological Sciences, Biophysicshttp://www.sciencedirect.com/science/article/pii/S258900421930029X |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mohammad S. Safari Zhiqing Wang Kunaal Tailor Anatoly B. Kolomeisky Jacinta C. Conrad Peter G. Vekilov |
spellingShingle |
Mohammad S. Safari Zhiqing Wang Kunaal Tailor Anatoly B. Kolomeisky Jacinta C. Conrad Peter G. Vekilov Anomalous Dense Liquid Condensates Host the Nucleation of Tumor Suppressor p53 Fibrils iScience |
author_facet |
Mohammad S. Safari Zhiqing Wang Kunaal Tailor Anatoly B. Kolomeisky Jacinta C. Conrad Peter G. Vekilov |
author_sort |
Mohammad S. Safari |
title |
Anomalous Dense Liquid Condensates Host the Nucleation of Tumor Suppressor p53 Fibrils |
title_short |
Anomalous Dense Liquid Condensates Host the Nucleation of Tumor Suppressor p53 Fibrils |
title_full |
Anomalous Dense Liquid Condensates Host the Nucleation of Tumor Suppressor p53 Fibrils |
title_fullStr |
Anomalous Dense Liquid Condensates Host the Nucleation of Tumor Suppressor p53 Fibrils |
title_full_unstemmed |
Anomalous Dense Liquid Condensates Host the Nucleation of Tumor Suppressor p53 Fibrils |
title_sort |
anomalous dense liquid condensates host the nucleation of tumor suppressor p53 fibrils |
publisher |
Elsevier |
series |
iScience |
issn |
2589-0042 |
publishDate |
2019-02-01 |
description |
Summary: About half of human cancers are associated with mutations of the tumor suppressor p53. Gained oncogenic functions of the mutants have been related to aggregation behaviors of wild-type and mutant p53. The thermodynamic and kinetic mechanisms of p53 aggregation are poorly understood. Here we find that wild-type p53 forms an anomalous liquid phase. The liquid condensates exhibit several behaviors beyond the scope of classical phase transition theories: their size, ca. 100 nm, is independent of the p53 concentration and decoupled from the protein mass held in the liquid phase. Furthermore, the liquid phase lacks constant solubility. The nucleation of p53 fibrils deviates from the accepted mechanism of sequential association of single solute molecules. We find that the liquid condensates serve as pre-assembled precursors of high p53 concentration that facilitate fibril assembly. Fibril nucleation hosted by precursors represents a novel biological pathway, which opens avenues to suppress protein fibrillation in aggregation diseases. : Chemistry; Biological Sciences; Biophysics Subject Areas: Chemistry, Biological Sciences, Biophysics |
url |
http://www.sciencedirect.com/science/article/pii/S258900421930029X |
work_keys_str_mv |
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