Sesamin suppresses LPS-induced microglial activation via regulation of TLR4 expression
Sesamin, one of the most abundant lignans in sesame seeds and oils, is well known for neuroprotective activity. However, its effects on toll-like-receptor 4 (TLR4), the key innate immune receptor implicated in microglia activation and neuroinflammation has not been reported. Our study demonstrated t...
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doaj-21242891e37942088181872dadb60c3a2021-04-30T07:14:34ZengElsevierJournal of Functional Foods1756-46462018-10-01493243Sesamin suppresses LPS-induced microglial activation via regulation of TLR4 expressionSasimol Udomruk0Chayanut Kaewmool1Peraphan Pothacharoen2Thanyaluck Phitak3Prachya Kongtawelert4Thailand Excellence Center for Tissue Engineering and Stem Cells, Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandThailand Excellence Center for Tissue Engineering and Stem Cells, Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandThailand Excellence Center for Tissue Engineering and Stem Cells, Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandThailand Excellence Center for Tissue Engineering and Stem Cells, Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandCorresponding author.; Thailand Excellence Center for Tissue Engineering and Stem Cells, Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandSesamin, one of the most abundant lignans in sesame seeds and oils, is well known for neuroprotective activity. However, its effects on toll-like-receptor 4 (TLR4), the key innate immune receptor implicated in microglia activation and neuroinflammation has not been reported. Our study demonstrated that sesamin significantly diminished LPS-stimulated TLR4 expression result in the reduction of nitric oxide (NO), prostaglandin E2 (PGE2) and pro-inflammatory cytokines (TNF-α, IL-1β and IL-6) in BV2 microglia by suppressing JNK and NF-κB pathway. While conditioned medium from LPS-stimulated microglia-induced PC12 cell death, sesamin pre-treatment on microglia abrogated the cytotoxic effects of pro-inflammatory mediators. Our result also demonstrated the direct effect of sesamin in ameliorating PC12 cell death induced by activated microglial-conditioned medium. These results suggested that sesamin alleviated inflammation-induced neurodegeneration via inhibition of TLR4 expression and microglial activation resulting in diminishing the neurotoxicity effect. Sesamin might be a potential agent for neurodegenerative diseases prevention.http://www.sciencedirect.com/science/article/pii/S1756464618304407SesaminToll-like receptor 4Pro-inflammatory mediatorsNeurodegeneration |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sasimol Udomruk Chayanut Kaewmool Peraphan Pothacharoen Thanyaluck Phitak Prachya Kongtawelert |
spellingShingle |
Sasimol Udomruk Chayanut Kaewmool Peraphan Pothacharoen Thanyaluck Phitak Prachya Kongtawelert Sesamin suppresses LPS-induced microglial activation via regulation of TLR4 expression Journal of Functional Foods Sesamin Toll-like receptor 4 Pro-inflammatory mediators Neurodegeneration |
author_facet |
Sasimol Udomruk Chayanut Kaewmool Peraphan Pothacharoen Thanyaluck Phitak Prachya Kongtawelert |
author_sort |
Sasimol Udomruk |
title |
Sesamin suppresses LPS-induced microglial activation via regulation of TLR4 expression |
title_short |
Sesamin suppresses LPS-induced microglial activation via regulation of TLR4 expression |
title_full |
Sesamin suppresses LPS-induced microglial activation via regulation of TLR4 expression |
title_fullStr |
Sesamin suppresses LPS-induced microglial activation via regulation of TLR4 expression |
title_full_unstemmed |
Sesamin suppresses LPS-induced microglial activation via regulation of TLR4 expression |
title_sort |
sesamin suppresses lps-induced microglial activation via regulation of tlr4 expression |
publisher |
Elsevier |
series |
Journal of Functional Foods |
issn |
1756-4646 |
publishDate |
2018-10-01 |
description |
Sesamin, one of the most abundant lignans in sesame seeds and oils, is well known for neuroprotective activity. However, its effects on toll-like-receptor 4 (TLR4), the key innate immune receptor implicated in microglia activation and neuroinflammation has not been reported. Our study demonstrated that sesamin significantly diminished LPS-stimulated TLR4 expression result in the reduction of nitric oxide (NO), prostaglandin E2 (PGE2) and pro-inflammatory cytokines (TNF-α, IL-1β and IL-6) in BV2 microglia by suppressing JNK and NF-κB pathway. While conditioned medium from LPS-stimulated microglia-induced PC12 cell death, sesamin pre-treatment on microglia abrogated the cytotoxic effects of pro-inflammatory mediators. Our result also demonstrated the direct effect of sesamin in ameliorating PC12 cell death induced by activated microglial-conditioned medium. These results suggested that sesamin alleviated inflammation-induced neurodegeneration via inhibition of TLR4 expression and microglial activation resulting in diminishing the neurotoxicity effect. Sesamin might be a potential agent for neurodegenerative diseases prevention. |
topic |
Sesamin Toll-like receptor 4 Pro-inflammatory mediators Neurodegeneration |
url |
http://www.sciencedirect.com/science/article/pii/S1756464618304407 |
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