Comparison of Biochemical and Molecular Methods for the Identification of Candida Species Causing Vulvovaginal Candidiasis and Recurring Vulvovaginal Candidiasis
Background and Aim: Vulvovaginal candidiasis (VVC) is a common clinical problem that affects most adult women at least once during their life time. Many also have frequently recurring yeast infections (RVVC) caused by Candida albicans and non albicans Candida species. We studied use of CHROMagar Can...
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doaj-212e28c616c74d3ea07c85738a992f112021-07-02T04:15:36ZengFarnameIranian Journal of Medical Microbiology1735-86122345-43422014-10-01834550Comparison of Biochemical and Molecular Methods for the Identification of Candida Species Causing Vulvovaginal Candidiasis and Recurring Vulvovaginal CandidiasisKambiz Diba0Atefeh Namaki1Haleh Ayatolahi2Haleh Hanifian3 Cellular and Molecular Research Center, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran Department of Gynecology, Arefian General Hospital, Urmia , Iran. Department of Gynecology, Motahari University Hospital, Urmia University of Medical Sciences, Urmia, Iran. Department of Mycology and Parasitology, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran. Background and Aim: Vulvovaginal candidiasis (VVC) is a common clinical problem that affects most adult women at least once during their life time. Many also have frequently recurring yeast infections (RVVC) caused by Candida albicans and non albicans Candida species. We studied use of CHROMagar Candida and PCR-RFLP to compare their differential ability and time consuming. Materials and Methods: Vaginal discharge samples of all women with clinical signs were cultured on CHROM agar Candida medium .After an overnight incubation at 37°C, growth coloration of Candida isolates compared with those of standard patterns. PCR-RFLP using restriction enzyme Msp I were used for the identification of more Candida isolates in the level of species.Results: Totally 192 vaginal discharge samples were obtained from the women with vulvovaginitis clinical signs. 90 patients had Candida vulvovaginitis, 11 case were RVVC. The Candida species which were identified included, C.albicans (78.8%), C.glabrata (7.7 %), C. parapsilosis (6.6%) , C. tropicalis (4.4 %) and C. krusei (2.2 %). Conclusions: We concluded that, use of PCR-RFLP is recommended for the identification of Candida species causing VVC and RVVC in accompany with the culture based methods, regarding to its differential power and speed.http://www.ijmm.ir/browse.php?a_code=A-10-89-1&slc_lang=en&sid=1Candida CHROMagar Molecular method |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kambiz Diba Atefeh Namaki Haleh Ayatolahi Haleh Hanifian |
spellingShingle |
Kambiz Diba Atefeh Namaki Haleh Ayatolahi Haleh Hanifian Comparison of Biochemical and Molecular Methods for the Identification of Candida Species Causing Vulvovaginal Candidiasis and Recurring Vulvovaginal Candidiasis Iranian Journal of Medical Microbiology Candida CHROMagar Molecular method |
author_facet |
Kambiz Diba Atefeh Namaki Haleh Ayatolahi Haleh Hanifian |
author_sort |
Kambiz Diba |
title |
Comparison of Biochemical and Molecular Methods for the Identification of Candida Species Causing Vulvovaginal Candidiasis and Recurring Vulvovaginal Candidiasis |
title_short |
Comparison of Biochemical and Molecular Methods for the Identification of Candida Species Causing Vulvovaginal Candidiasis and Recurring Vulvovaginal Candidiasis |
title_full |
Comparison of Biochemical and Molecular Methods for the Identification of Candida Species Causing Vulvovaginal Candidiasis and Recurring Vulvovaginal Candidiasis |
title_fullStr |
Comparison of Biochemical and Molecular Methods for the Identification of Candida Species Causing Vulvovaginal Candidiasis and Recurring Vulvovaginal Candidiasis |
title_full_unstemmed |
Comparison of Biochemical and Molecular Methods for the Identification of Candida Species Causing Vulvovaginal Candidiasis and Recurring Vulvovaginal Candidiasis |
title_sort |
comparison of biochemical and molecular methods for the identification of candida species causing vulvovaginal candidiasis and recurring vulvovaginal candidiasis |
publisher |
Farname |
series |
Iranian Journal of Medical Microbiology |
issn |
1735-8612 2345-4342 |
publishDate |
2014-10-01 |
description |
Background
and Aim: Vulvovaginal candidiasis (VVC) is a common clinical problem
that affects most adult women at least once during their life time. Many also
have frequently recurring yeast infections (RVVC) caused by Candida albicans
and non albicans Candida species. We studied use of CHROMagar Candida
and PCR-RFLP to compare their differential ability and time consuming. Materials
and Methods: Vaginal discharge samples of all
women with clinical signs were cultured on CHROM agar Candida medium .After an
overnight incubation at 37°C, growth coloration of Candida isolates compared
with those of standard patterns. PCR-RFLP using restriction enzyme Msp I
were used for the identification of more
Candida isolates in the level of species.Results:
Totally
192 vaginal discharge samples were obtained from the women with vulvovaginitis
clinical signs. 90 patients had Candida vulvovaginitis, 11 case were RVVC. The Candida
species which were identified included, C.albicans (78.8%), C.glabrata
(7.7 %), C. parapsilosis (6.6%) , C. tropicalis (4.4 %) and C. krusei
(2.2 %).
Conclusions:
We
concluded that, use of PCR-RFLP is recommended for the identification of Candida
species causing VVC and RVVC in accompany with the culture based methods,
regarding to its differential power and speed. |
topic |
Candida CHROMagar Molecular method |
url |
http://www.ijmm.ir/browse.php?a_code=A-10-89-1&slc_lang=en&sid=1 |
work_keys_str_mv |
AT kambizdiba comparisonofbiochemicalandmolecularmethodsfortheidentificationofcandidaspeciescausingvulvovaginalcandidiasisandrecurringvulvovaginalcandidiasis AT atefehnamaki comparisonofbiochemicalandmolecularmethodsfortheidentificationofcandidaspeciescausingvulvovaginalcandidiasisandrecurringvulvovaginalcandidiasis AT halehayatolahi comparisonofbiochemicalandmolecularmethodsfortheidentificationofcandidaspeciescausingvulvovaginalcandidiasisandrecurringvulvovaginalcandidiasis AT halehhanifian comparisonofbiochemicalandmolecularmethodsfortheidentificationofcandidaspeciescausingvulvovaginalcandidiasisandrecurringvulvovaginalcandidiasis |
_version_ |
1721340441426657280 |