Development of multiplex-PCR assay for rapid detection of <em>Candida spp.</em>
<p><strong>Aim</strong> <em>Candida spp.</em> infection commonly occur in immunocompromised patients. Biochemical assay for identification of <em>Candida spp.</em> is time-consuming and shows many undetermined results. Specific detection for antibody, antige...
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Faculty of Medicine Universitas Indonesia
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doaj-214c85c9aa6044a293cd6256da768f1d2020-11-25T01:15:43ZengFaculty of Medicine Universitas Indonesia Medical Journal of Indonesia0853-17732252-80832010-05-0119283710.13181/mji.v19i2.387384Development of multiplex-PCR assay for rapid detection of <em>Candida spp.</em>Ni Made A. TariniMardiastuti H. WahidFera IbrahimAndi YasmonSamsuridjal Djauzi<p><strong>Aim</strong> <em>Candida spp.</em> infection commonly occur in immunocompromised patients. Biochemical assay for identification of <em>Candida spp.</em> is time-consuming and shows many undetermined results. Specific detection for antibody, antigen and metabolites of <em>Candida spp.</em> had low sensitivity and specificity. In this study, we developed a rapid diagnostic method, Multiplex-PCR, to identify <em>Candida spp.</em></p><p><strong>Methods</strong> Five <em>Candida spp.</em> isolates were cultured, identifi ed with germ tube and API® 20 C AUX (BioMerieux® SA) kit. Furthermore, DNA was purified by QIAamp DNA mini (Qiagen®) kit for Multiplex-PCR assay.</p><p><strong>Results</strong> DNA detection limit by Multiplex-PCR assays for <em>C. albicans, C. tropicalis, C. parapsilosis, C. krusei</em> and <em>C. glabrata</em> were 4 pg, 0.98 pg, 0.98 pg, 0.5 pg and 16 pg respectively. This assay was also more sensitive than culture in that Multiplex-PCR could detect 2.6-2.9 x 100 CFU/ml, whereas culture 2.6-2.9 x 102 CFU/ml.</p><p><strong>Conclusion</strong> Multiplex-PCR is much more sensitive than culture and thus, can be recommended as a sensitive and specific assay for identification of <em>Candida spp.</em> <em><strong>(Med J Indones 2010; 19:83-7)</strong></em></p><p><strong>Keywords:</strong> <em>Candida spp., multiplex-PCR</em></p>http://mji.ui.ac.id/journal/index.php/mji/article/view/387 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ni Made A. Tarini Mardiastuti H. Wahid Fera Ibrahim Andi Yasmon Samsuridjal Djauzi |
spellingShingle |
Ni Made A. Tarini Mardiastuti H. Wahid Fera Ibrahim Andi Yasmon Samsuridjal Djauzi Development of multiplex-PCR assay for rapid detection of <em>Candida spp.</em> Medical Journal of Indonesia |
author_facet |
Ni Made A. Tarini Mardiastuti H. Wahid Fera Ibrahim Andi Yasmon Samsuridjal Djauzi |
author_sort |
Ni Made A. Tarini |
title |
Development of multiplex-PCR assay for rapid detection of <em>Candida spp.</em> |
title_short |
Development of multiplex-PCR assay for rapid detection of <em>Candida spp.</em> |
title_full |
Development of multiplex-PCR assay for rapid detection of <em>Candida spp.</em> |
title_fullStr |
Development of multiplex-PCR assay for rapid detection of <em>Candida spp.</em> |
title_full_unstemmed |
Development of multiplex-PCR assay for rapid detection of <em>Candida spp.</em> |
title_sort |
development of multiplex-pcr assay for rapid detection of <em>candida spp.</em> |
publisher |
Faculty of Medicine Universitas Indonesia |
series |
Medical Journal of Indonesia |
issn |
0853-1773 2252-8083 |
publishDate |
2010-05-01 |
description |
<p><strong>Aim</strong> <em>Candida spp.</em> infection commonly occur in immunocompromised patients. Biochemical assay for identification of <em>Candida spp.</em> is time-consuming and shows many undetermined results. Specific detection for antibody, antigen and metabolites of <em>Candida spp.</em> had low sensitivity and specificity. In this study, we developed a rapid diagnostic method, Multiplex-PCR, to identify <em>Candida spp.</em></p><p><strong>Methods</strong> Five <em>Candida spp.</em> isolates were cultured, identifi ed with germ tube and API® 20 C AUX (BioMerieux® SA) kit. Furthermore, DNA was purified by QIAamp DNA mini (Qiagen®) kit for Multiplex-PCR assay.</p><p><strong>Results</strong> DNA detection limit by Multiplex-PCR assays for <em>C. albicans, C. tropicalis, C. parapsilosis, C. krusei</em> and <em>C. glabrata</em> were 4 pg, 0.98 pg, 0.98 pg, 0.5 pg and 16 pg respectively. This assay was also more sensitive than culture in that Multiplex-PCR could detect 2.6-2.9 x 100 CFU/ml, whereas culture 2.6-2.9 x 102 CFU/ml.</p><p><strong>Conclusion</strong> Multiplex-PCR is much more sensitive than culture and thus, can be recommended as a sensitive and specific assay for identification of <em>Candida spp.</em> <em><strong>(Med J Indones 2010; 19:83-7)</strong></em></p><p><strong>Keywords:</strong> <em>Candida spp., multiplex-PCR</em></p> |
url |
http://mji.ui.ac.id/journal/index.php/mji/article/view/387 |
work_keys_str_mv |
AT nimadeatarini developmentofmultiplexpcrassayforrapiddetectionofemcandidasppem AT mardiastutihwahid developmentofmultiplexpcrassayforrapiddetectionofemcandidasppem AT feraibrahim developmentofmultiplexpcrassayforrapiddetectionofemcandidasppem AT andiyasmon developmentofmultiplexpcrassayforrapiddetectionofemcandidasppem AT samsuridjaldjauzi developmentofmultiplexpcrassayforrapiddetectionofemcandidasppem |
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