Biosensing Amplification by Hybridization Chain Reaction on Phase-Sensitive Surface Plasmon Resonance

Surface Plasmon Resonance (SPR) is widely used in biological and chemical sensing with fascinating properties. However, the application of SPR to detect trace targets is hampered by non-specific binding and poor signal. A variety of approaches for amplification have been explored to overcome this de...

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Main Authors: Ching-Hsu Yang, Tzu-Heng Wu, Chia-Chen Chang, Hui-Yun Lo, Hui-Wen Liu, Nien-Tsu Huang, Chii-Wann Lin
Format: Article
Language:English
Published: MDPI AG 2021-03-01
Series:Biosensors
Subjects:
Online Access:https://www.mdpi.com/2079-6374/11/3/75
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spelling doaj-2152b5dc12094f6d88647fb4f3c783fc2021-03-07T00:03:56ZengMDPI AGBiosensors2079-63742021-03-0111757510.3390/bios11030075Biosensing Amplification by Hybridization Chain Reaction on Phase-Sensitive Surface Plasmon ResonanceChing-Hsu Yang0Tzu-Heng Wu1Chia-Chen Chang2Hui-Yun Lo3Hui-Wen Liu4Nien-Tsu Huang5Chii-Wann Lin6Graduate Institute of Bioelectronics and Bioinformatics, National Taiwan University, Taipei 106, TaiwanGraduate Institute of Bioelectronics and Bioinformatics, National Taiwan University, Taipei 106, TaiwanDepartment of Medical Biotechnology and Laboratory Sciences, College of Medicine, Chang Gung University, Taoyuan 333, TaiwanDepartment of Biomedical Engineering, National Taiwan University, Taipei 106, TaiwanDepartment of Biomedical Engineering, National Taiwan University, Taipei 106, TaiwanGraduate Institute of Bioelectronics and Bioinformatics, National Taiwan University, Taipei 106, TaiwanDepartment of Biomedical Engineering, National Taiwan University, Taipei 106, TaiwanSurface Plasmon Resonance (SPR) is widely used in biological and chemical sensing with fascinating properties. However, the application of SPR to detect trace targets is hampered by non-specific binding and poor signal. A variety of approaches for amplification have been explored to overcome this deficiency including DNA aptamers as versatile target detection tools. Hybridization chain reaction (HCR) is a high-efficiency enzyme-free DNA amplification method operated at room temperature, in which two stable species of DNA hairpins coexist in solution until the introduction of the initiator strand triggers a cascade of hybridization events. At an optimal salt condition, as the concentrations of H1 and H2 increased, the HCR signals were enhanced, leading to signal amplification reaching up to 6.5-fold of the detection measure at 30 min. This feature enables DNA to act as an amplifying transducer for biosensing applications to provide an enzyme-free alternative that can easily detect complex DNA sequences. Improvement of more diverse recognition events can be achieved by integrating HCR with a phase-sensitive SPR (pSPR)-tested aptamer stimulus. This work seeks to establish pSPR aptamer system for highly informative sensing by means of an amplification HCR. Thus, combining pSPR and HCR technologies provide an expandable platform for sensitive biosensing.https://www.mdpi.com/2079-6374/11/3/75aptamerhybridization chain reactionphase-sensitive surface plasmon resonance (pSPR) biosensor
collection DOAJ
language English
format Article
sources DOAJ
author Ching-Hsu Yang
Tzu-Heng Wu
Chia-Chen Chang
Hui-Yun Lo
Hui-Wen Liu
Nien-Tsu Huang
Chii-Wann Lin
spellingShingle Ching-Hsu Yang
Tzu-Heng Wu
Chia-Chen Chang
Hui-Yun Lo
Hui-Wen Liu
Nien-Tsu Huang
Chii-Wann Lin
Biosensing Amplification by Hybridization Chain Reaction on Phase-Sensitive Surface Plasmon Resonance
Biosensors
aptamer
hybridization chain reaction
phase-sensitive surface plasmon resonance (pSPR) biosensor
author_facet Ching-Hsu Yang
Tzu-Heng Wu
Chia-Chen Chang
Hui-Yun Lo
Hui-Wen Liu
Nien-Tsu Huang
Chii-Wann Lin
author_sort Ching-Hsu Yang
title Biosensing Amplification by Hybridization Chain Reaction on Phase-Sensitive Surface Plasmon Resonance
title_short Biosensing Amplification by Hybridization Chain Reaction on Phase-Sensitive Surface Plasmon Resonance
title_full Biosensing Amplification by Hybridization Chain Reaction on Phase-Sensitive Surface Plasmon Resonance
title_fullStr Biosensing Amplification by Hybridization Chain Reaction on Phase-Sensitive Surface Plasmon Resonance
title_full_unstemmed Biosensing Amplification by Hybridization Chain Reaction on Phase-Sensitive Surface Plasmon Resonance
title_sort biosensing amplification by hybridization chain reaction on phase-sensitive surface plasmon resonance
publisher MDPI AG
series Biosensors
issn 2079-6374
publishDate 2021-03-01
description Surface Plasmon Resonance (SPR) is widely used in biological and chemical sensing with fascinating properties. However, the application of SPR to detect trace targets is hampered by non-specific binding and poor signal. A variety of approaches for amplification have been explored to overcome this deficiency including DNA aptamers as versatile target detection tools. Hybridization chain reaction (HCR) is a high-efficiency enzyme-free DNA amplification method operated at room temperature, in which two stable species of DNA hairpins coexist in solution until the introduction of the initiator strand triggers a cascade of hybridization events. At an optimal salt condition, as the concentrations of H1 and H2 increased, the HCR signals were enhanced, leading to signal amplification reaching up to 6.5-fold of the detection measure at 30 min. This feature enables DNA to act as an amplifying transducer for biosensing applications to provide an enzyme-free alternative that can easily detect complex DNA sequences. Improvement of more diverse recognition events can be achieved by integrating HCR with a phase-sensitive SPR (pSPR)-tested aptamer stimulus. This work seeks to establish pSPR aptamer system for highly informative sensing by means of an amplification HCR. Thus, combining pSPR and HCR technologies provide an expandable platform for sensitive biosensing.
topic aptamer
hybridization chain reaction
phase-sensitive surface plasmon resonance (pSPR) biosensor
url https://www.mdpi.com/2079-6374/11/3/75
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AT huiyunlo biosensingamplificationbyhybridizationchainreactiononphasesensitivesurfaceplasmonresonance
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