Germ-line recombination activity of the widely used hGFAP-Cre and nestin-Cre transgenes.
Herein we demonstrate with PCR, immunodetection and reporter gene approaches that the widely used human Glial Fibrillary Acidic Protein (hGFAP)-Cre transgene exhibits spontaneous germ-line recombination activity in leading to deletion in brain, heart and tail tissue with high frequency. The ectopic...
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doaj-2155fe001cfc497b98c178e24ea912b62020-11-25T00:47:15ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01812e8281810.1371/journal.pone.0082818Germ-line recombination activity of the widely used hGFAP-Cre and nestin-Cre transgenes.Jiong ZhangPavel DublinStephanie GriemsmannAlexandra KleinRalph BrehmPeter BednerBernd K FleischmannChristian SteinhäuserMartin TheisHerein we demonstrate with PCR, immunodetection and reporter gene approaches that the widely used human Glial Fibrillary Acidic Protein (hGFAP)-Cre transgene exhibits spontaneous germ-line recombination activity in leading to deletion in brain, heart and tail tissue with high frequency. The ectopic activity of hGFAP-Cre requires a rigorous control. We likewise observed that a second widely used nestin-Cre transgene shows germ-line deletion. Here we describe procedures to identify mice with germ-line recombination mediated by the hGFAP-Cre and nestin-Cre transgenes. Such control is essential to avoid pleiotropic effects due to germ-line deletion of loxP-flanked target genes and to maintain the CNS-restricted deletion status in transgenic mouse colonies.http://europepmc.org/articles/PMC3857304?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jiong Zhang Pavel Dublin Stephanie Griemsmann Alexandra Klein Ralph Brehm Peter Bedner Bernd K Fleischmann Christian Steinhäuser Martin Theis |
spellingShingle |
Jiong Zhang Pavel Dublin Stephanie Griemsmann Alexandra Klein Ralph Brehm Peter Bedner Bernd K Fleischmann Christian Steinhäuser Martin Theis Germ-line recombination activity of the widely used hGFAP-Cre and nestin-Cre transgenes. PLoS ONE |
author_facet |
Jiong Zhang Pavel Dublin Stephanie Griemsmann Alexandra Klein Ralph Brehm Peter Bedner Bernd K Fleischmann Christian Steinhäuser Martin Theis |
author_sort |
Jiong Zhang |
title |
Germ-line recombination activity of the widely used hGFAP-Cre and nestin-Cre transgenes. |
title_short |
Germ-line recombination activity of the widely used hGFAP-Cre and nestin-Cre transgenes. |
title_full |
Germ-line recombination activity of the widely used hGFAP-Cre and nestin-Cre transgenes. |
title_fullStr |
Germ-line recombination activity of the widely used hGFAP-Cre and nestin-Cre transgenes. |
title_full_unstemmed |
Germ-line recombination activity of the widely used hGFAP-Cre and nestin-Cre transgenes. |
title_sort |
germ-line recombination activity of the widely used hgfap-cre and nestin-cre transgenes. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
Herein we demonstrate with PCR, immunodetection and reporter gene approaches that the widely used human Glial Fibrillary Acidic Protein (hGFAP)-Cre transgene exhibits spontaneous germ-line recombination activity in leading to deletion in brain, heart and tail tissue with high frequency. The ectopic activity of hGFAP-Cre requires a rigorous control. We likewise observed that a second widely used nestin-Cre transgene shows germ-line deletion. Here we describe procedures to identify mice with germ-line recombination mediated by the hGFAP-Cre and nestin-Cre transgenes. Such control is essential to avoid pleiotropic effects due to germ-line deletion of loxP-flanked target genes and to maintain the CNS-restricted deletion status in transgenic mouse colonies. |
url |
http://europepmc.org/articles/PMC3857304?pdf=render |
work_keys_str_mv |
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