Germ-line recombination activity of the widely used hGFAP-Cre and nestin-Cre transgenes.
Herein we demonstrate with PCR, immunodetection and reporter gene approaches that the widely used human Glial Fibrillary Acidic Protein (hGFAP)-Cre transgene exhibits spontaneous germ-line recombination activity in leading to deletion in brain, heart and tail tissue with high frequency. The ectopic...
Main Authors: | Jiong Zhang, Pavel Dublin, Stephanie Griemsmann, Alexandra Klein, Ralph Brehm, Peter Bedner, Bernd K Fleischmann, Christian Steinhäuser, Martin Theis |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2013-01-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3857304?pdf=render |
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