Direct and indirect methods of determining DNA nucleotide sequences in microorganisms
Typing of bacterial populations and identification of microorganisms are the overriding priorities in the field of prophylaxis, diagnosis and treatment of bacterial infections. Over the recent two decades rapidly developing approaches to molecular typing of bacterial populations have become an essen...
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Ministry of Health of the Russian Federation. Federal State Budgetary Institution «Scientific Centre for Expert Evaluation of Medicinal Products»
2018-02-01
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Series: | Биопрепараты: Профилактика, диагностика, лечение |
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Online Access: | https://www.biopreparations.ru/jour/article/view/9 |
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doaj-218ff3e24fd8452ab4c4814d845233702021-07-28T14:03:33ZrusMinistry of Health of the Russian Federation. Federal State Budgetary Institution «Scientific Centre for Expert Evaluation of Medicinal Products» Биопрепараты: Профилактика, диагностика, лечение2221-996X2619-11562018-02-01029149Direct and indirect methods of determining DNA nucleotide sequences in microorganismsR. A. Volkova0E. S. Skolotneva1E. V. Elbert2E. D. Mytsa3V. A. Merkulov4V. P. Bondarev5I. V. Borisevich6Federal State Budgetary Institution «Scientific Center on Expertise of Medical Application Products» of the Ministry of Health of the Russian FederationM.V. Lomonosov Moscow State UniversityFederal State Budgetary Institution «Scientific Center on Expertise of Medical Application Products» of the Ministry of Health of the Russian FederationFederal State Budgetary Institution «Scientific Center on Expertise of Medical Application Products» of the Ministry of Health of the Russian FederationFederal State Budgetary Institution «Scientific Center on Expertise of Medical Application Products» of the Ministry of Health of the Russian FederationFederal State Budgetary Institution «Scientific Center on Expertise of Medical Application Products» of the Ministry of Health of the Russian FederationFederal State Budgetary Institution «Scientific Center on Expertise of Medical Application Products» of the Ministry of Health of the Russian FederationTyping of bacterial populations and identification of microorganisms are the overriding priorities in the field of prophylaxis, diagnosis and treatment of bacterial infections. Over the recent two decades rapidly developing approaches to molecular typing of bacterial populations have become an essential tool for the mentioned task. The information encoded in nucleic acids is more complete as compared to other characteristics of an organism. It can be obtained by either direct determination of genome sequences or by using a variety of techniques, that allow to indirectly assess the nucleotide composition in a representative genome locus of a microorganism under study. The article provides an overview of the literature data on direct and indirect methods of determining nucleotide composition of DNA sequences in microorganisms (multiple locus sequence typing, multiple sequeneed sites typing, denaturing gel electrophoresis, high-sensitivity melting curve analysis, DNA macro(micro)arrays).https://www.biopreparations.ru/jour/article/view/9генотипированиемикроорганизмымолекулярно-генетические методысеквенированиеднк макро(микро) чипыgenotypingmicroorganismsmolecular methodsdna sequencingdna macro(micro)arrays |
collection |
DOAJ |
language |
Russian |
format |
Article |
sources |
DOAJ |
author |
R. A. Volkova E. S. Skolotneva E. V. Elbert E. D. Mytsa V. A. Merkulov V. P. Bondarev I. V. Borisevich |
spellingShingle |
R. A. Volkova E. S. Skolotneva E. V. Elbert E. D. Mytsa V. A. Merkulov V. P. Bondarev I. V. Borisevich Direct and indirect methods of determining DNA nucleotide sequences in microorganisms Биопрепараты: Профилактика, диагностика, лечение генотипирование микроорганизмы молекулярно-генетические методы секвенирование днк макро(микро) чипы genotyping microorganisms molecular methods dna sequencing dna macro(micro)arrays |
author_facet |
R. A. Volkova E. S. Skolotneva E. V. Elbert E. D. Mytsa V. A. Merkulov V. P. Bondarev I. V. Borisevich |
author_sort |
R. A. Volkova |
title |
Direct and indirect methods of determining DNA nucleotide sequences in microorganisms |
title_short |
Direct and indirect methods of determining DNA nucleotide sequences in microorganisms |
title_full |
Direct and indirect methods of determining DNA nucleotide sequences in microorganisms |
title_fullStr |
Direct and indirect methods of determining DNA nucleotide sequences in microorganisms |
title_full_unstemmed |
Direct and indirect methods of determining DNA nucleotide sequences in microorganisms |
title_sort |
direct and indirect methods of determining dna nucleotide sequences in microorganisms |
publisher |
Ministry of Health of the Russian Federation. Federal State Budgetary Institution «Scientific Centre for Expert Evaluation of Medicinal Products» |
series |
Биопрепараты: Профилактика, диагностика, лечение |
issn |
2221-996X 2619-1156 |
publishDate |
2018-02-01 |
description |
Typing of bacterial populations and identification of microorganisms are the overriding priorities in the field of prophylaxis, diagnosis and treatment of bacterial infections. Over the recent two decades rapidly developing approaches to molecular typing of bacterial populations have become an essential tool for the mentioned task. The information encoded in nucleic acids is more complete as compared to other characteristics of an organism. It can be obtained by either direct determination of genome sequences or by using a variety of techniques, that allow to indirectly assess the nucleotide composition in a representative genome locus of a microorganism under study. The article provides an overview of the literature data on direct and indirect methods of determining nucleotide composition of DNA sequences in microorganisms (multiple locus sequence typing, multiple sequeneed sites typing, denaturing gel electrophoresis, high-sensitivity melting curve analysis, DNA macro(micro)arrays). |
topic |
генотипирование микроорганизмы молекулярно-генетические методы секвенирование днк макро(микро) чипы genotyping microorganisms molecular methods dna sequencing dna macro(micro)arrays |
url |
https://www.biopreparations.ru/jour/article/view/9 |
work_keys_str_mv |
AT ravolkova directandindirectmethodsofdeterminingdnanucleotidesequencesinmicroorganisms AT esskolotneva directandindirectmethodsofdeterminingdnanucleotidesequencesinmicroorganisms AT evelbert directandindirectmethodsofdeterminingdnanucleotidesequencesinmicroorganisms AT edmytsa directandindirectmethodsofdeterminingdnanucleotidesequencesinmicroorganisms AT vamerkulov directandindirectmethodsofdeterminingdnanucleotidesequencesinmicroorganisms AT vpbondarev directandindirectmethodsofdeterminingdnanucleotidesequencesinmicroorganisms AT ivborisevich directandindirectmethodsofdeterminingdnanucleotidesequencesinmicroorganisms |
_version_ |
1721268669868146688 |