Obtaining patient-derived cancer organoid cultures via fine-needle aspiration

Summary: Patient-derived tumor organoid cultures are an essential and innovative methodology for translational research. However, current techniques to establish these cultures are cumbersome, expensive, and often require irreplaceable clinical tissue from surgery or core biopsies. Fine-needle aspir...

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Main Authors:	Courtney J. Phifer, Kensey N. Bergdorf, Matthew E. Bechard, Anna Vilgelm, Naira Baregamian, Oliver G. McDonald, Ethan Lee, Vivian L. Weiss
Format:	Article
Language:	English
Published:	Elsevier 2021-03-01
Series:	STAR Protocols
Subjects:	cell culture cell isolation cancer organoids
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166720302070

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spelling	doaj-2191ce4fe9eb4249bf0083cec3ced77e2021-03-22T12:52:42ZengElsevierSTAR Protocols2666-16672021-03-0121100220Obtaining patient-derived cancer organoid cultures via fine-needle aspirationCourtney J. Phifer0Kensey N. Bergdorf1Matthew E. Bechard2Anna Vilgelm3Naira Baregamian4Oliver G. McDonald5Ethan Lee6Vivian L. Weiss7Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USADepartment of Pharmacology, Vanderbilt University, Nashville, TN 37232, USADepartment of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USADepartment of Pathology, Ohio State University, Columbus, OH 43210, USADepartment of Surgery, Vanderbilt University Medical Center, Nashville, TN 37232, USA; Vanderbilt Ingram Cancer Center, Vanderbilt University Medical Center, Nashville, TN 37232, USADepartment of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA; Vanderbilt Ingram Cancer Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA; Epithelial Biology Center, Vanderbilt University Medical Center, Nashville, TN 37232, USADepartment of Pharmacology, Vanderbilt University, Nashville, TN 37232, USA; Vanderbilt Ingram Cancer Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA; Department of Cell and Developmental Biology, Nashville, TN 37232, USADepartment of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA; Department of Pharmacology, Vanderbilt University, Nashville, TN 37232, USA; Vanderbilt Ingram Cancer Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA; Corresponding authorSummary: Patient-derived tumor organoid cultures are an essential and innovative methodology for translational research. However, current techniques to establish these cultures are cumbersome, expensive, and often require irreplaceable clinical tissue from surgery or core biopsies. Fine-needle aspiration (FNA) provides a minimally invasive biopsy technique commonly performed in clinical settings. Here, we provide a protocol for FNA. We have found that FNA provides a cost-effective, rapid, and streamlined method for tissue acquisition for cancer organoid culture.For complete details on the use and execution of this protocol, please refer to Lee et al. (2020) and Vilgelm et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166720302070cell culturecell isolationcancerorganoids
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Courtney J. Phifer Kensey N. Bergdorf Matthew E. Bechard Anna Vilgelm Naira Baregamian Oliver G. McDonald Ethan Lee Vivian L. Weiss
spellingShingle	Courtney J. Phifer Kensey N. Bergdorf Matthew E. Bechard Anna Vilgelm Naira Baregamian Oliver G. McDonald Ethan Lee Vivian L. Weiss Obtaining patient-derived cancer organoid cultures via fine-needle aspiration STAR Protocols cell culture cell isolation cancer organoids
author_facet	Courtney J. Phifer Kensey N. Bergdorf Matthew E. Bechard Anna Vilgelm Naira Baregamian Oliver G. McDonald Ethan Lee Vivian L. Weiss
author_sort	Courtney J. Phifer
title	Obtaining patient-derived cancer organoid cultures via fine-needle aspiration
title_short	Obtaining patient-derived cancer organoid cultures via fine-needle aspiration
title_full	Obtaining patient-derived cancer organoid cultures via fine-needle aspiration
title_fullStr	Obtaining patient-derived cancer organoid cultures via fine-needle aspiration
title_full_unstemmed	Obtaining patient-derived cancer organoid cultures via fine-needle aspiration
title_sort	obtaining patient-derived cancer organoid cultures via fine-needle aspiration
publisher	Elsevier
series	STAR Protocols
issn	2666-1667
publishDate	2021-03-01
description	Summary: Patient-derived tumor organoid cultures are an essential and innovative methodology for translational research. However, current techniques to establish these cultures are cumbersome, expensive, and often require irreplaceable clinical tissue from surgery or core biopsies. Fine-needle aspiration (FNA) provides a minimally invasive biopsy technique commonly performed in clinical settings. Here, we provide a protocol for FNA. We have found that FNA provides a cost-effective, rapid, and streamlined method for tissue acquisition for cancer organoid culture.For complete details on the use and execution of this protocol, please refer to Lee et al. (2020) and Vilgelm et al. (2020).
topic	cell culture cell isolation cancer organoids
url	http://www.sciencedirect.com/science/article/pii/S2666166720302070
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Obtaining patient-derived cancer organoid cultures via fine-needle aspiration

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