Traditional Uyghur medicine Quercus infectoria galls water extract triggers apoptosis and autophagic cell death in colorectal cancer cells
Abstract Background The water extract of Quercuse infectoria galls (QIG) is the active ingredient of Uyghur medicine Xipayi Kui Jie’an (KJA) which has promising therapeutic effects on Ulcerative Colitis (UC) as an alternative medicine. Considering the relationship between UC and the development of c...
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doaj-219876ccbab8494094ef4fe4e50844e52020-12-06T12:28:27ZengBMCBMC Complementary Medicine and Therapies2662-76712020-12-0120111210.1186/s12906-020-03167-0Traditional Uyghur medicine Quercus infectoria galls water extract triggers apoptosis and autophagic cell death in colorectal cancer cellsHui Zhang0Yongbing Wang1Jiayang Liu2Kudelaidi Kuerban3Jian Li4Mubarak Iminjan5Li Ye6Department of Biological Medicines & Shanghai Engineering Research Center of Immunotherapeutics, School of Pharmacy, Fudan UniversityPudong New Area People’s Hospital Affiliated to Shanghai University of Medicine and Health SciencesDepartment of Biological Medicines & Shanghai Engineering Research Center of Immunotherapeutics, School of Pharmacy, Fudan UniversityDepartment of Biological Medicines & Shanghai Engineering Research Center of Immunotherapeutics, School of Pharmacy, Fudan UniversityEndoscopy Center, Minhang Branch of Zhongshan Hospital, Fudan UniversityDepartment of pharmaceutical and physical chemistry, College of pharmacy, Xinjiang Medical UniversityDepartment of Biological Medicines & Shanghai Engineering Research Center of Immunotherapeutics, School of Pharmacy, Fudan UniversityAbstract Background The water extract of Quercuse infectoria galls (QIG) is the active ingredient of Uyghur medicine Xipayi Kui Jie’an (KJA) which has promising therapeutic effects on Ulcerative Colitis (UC) as an alternative medicine. Considering the relationship between UC and the development of colorectal cancer (CRC), the present work aims to explore the direct anti-CRC activity of QIG extract. Methods CCK8 assay and flow cytometry were used to detect cytotoxicity and apoptosis. Transmission electron microscopy (TEM), flow cytometry, laser confocal and western blotting were performed to examine autophagy. We also adopted Reactive Oxygen Assay kit, as well as transwell and wound healing tests to study the underlying mechanism of QIG against CRC cells. Results First, we found that QIG extract could suppress the viability of CRC cells and trigger caspases-dependent apoptosis. Subsequently, we proved for the first time that QIG extract also triggered autophagic cell death in CRC cells, which together with apoptosis contributed to the cytotoxic effect on CRC cells. Further investigation revealed that QIG-induced cytotoxicity associated with intracellular ROS accumulation which could suppress the AKT/mTOR signaling pathway, and then induce autophagy and inhibit cell growth. Besides, Erk signaling pathway was also involved in the process of autophagic cell death. Moreover, QIG extract also influenced EMT process and inhibited CRC cell migration. Conclusion Altogether, this study provides a basis for the utilization of QIG as an alternative medicine for CRC prevention and treatment.https://doi.org/10.1186/s12906-020-03167-0Quercuse infectoria gallsColorectal cancerApoptosisAutophagyReactive oxygen species |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hui Zhang Yongbing Wang Jiayang Liu Kudelaidi Kuerban Jian Li Mubarak Iminjan Li Ye |
spellingShingle |
Hui Zhang Yongbing Wang Jiayang Liu Kudelaidi Kuerban Jian Li Mubarak Iminjan Li Ye Traditional Uyghur medicine Quercus infectoria galls water extract triggers apoptosis and autophagic cell death in colorectal cancer cells BMC Complementary Medicine and Therapies Quercuse infectoria galls Colorectal cancer Apoptosis Autophagy Reactive oxygen species |
author_facet |
Hui Zhang Yongbing Wang Jiayang Liu Kudelaidi Kuerban Jian Li Mubarak Iminjan Li Ye |
author_sort |
Hui Zhang |
title |
Traditional Uyghur medicine Quercus infectoria galls water extract triggers apoptosis and autophagic cell death in colorectal cancer cells |
title_short |
Traditional Uyghur medicine Quercus infectoria galls water extract triggers apoptosis and autophagic cell death in colorectal cancer cells |
title_full |
Traditional Uyghur medicine Quercus infectoria galls water extract triggers apoptosis and autophagic cell death in colorectal cancer cells |
title_fullStr |
Traditional Uyghur medicine Quercus infectoria galls water extract triggers apoptosis and autophagic cell death in colorectal cancer cells |
title_full_unstemmed |
Traditional Uyghur medicine Quercus infectoria galls water extract triggers apoptosis and autophagic cell death in colorectal cancer cells |
title_sort |
traditional uyghur medicine quercus infectoria galls water extract triggers apoptosis and autophagic cell death in colorectal cancer cells |
publisher |
BMC |
series |
BMC Complementary Medicine and Therapies |
issn |
2662-7671 |
publishDate |
2020-12-01 |
description |
Abstract Background The water extract of Quercuse infectoria galls (QIG) is the active ingredient of Uyghur medicine Xipayi Kui Jie’an (KJA) which has promising therapeutic effects on Ulcerative Colitis (UC) as an alternative medicine. Considering the relationship between UC and the development of colorectal cancer (CRC), the present work aims to explore the direct anti-CRC activity of QIG extract. Methods CCK8 assay and flow cytometry were used to detect cytotoxicity and apoptosis. Transmission electron microscopy (TEM), flow cytometry, laser confocal and western blotting were performed to examine autophagy. We also adopted Reactive Oxygen Assay kit, as well as transwell and wound healing tests to study the underlying mechanism of QIG against CRC cells. Results First, we found that QIG extract could suppress the viability of CRC cells and trigger caspases-dependent apoptosis. Subsequently, we proved for the first time that QIG extract also triggered autophagic cell death in CRC cells, which together with apoptosis contributed to the cytotoxic effect on CRC cells. Further investigation revealed that QIG-induced cytotoxicity associated with intracellular ROS accumulation which could suppress the AKT/mTOR signaling pathway, and then induce autophagy and inhibit cell growth. Besides, Erk signaling pathway was also involved in the process of autophagic cell death. Moreover, QIG extract also influenced EMT process and inhibited CRC cell migration. Conclusion Altogether, this study provides a basis for the utilization of QIG as an alternative medicine for CRC prevention and treatment. |
topic |
Quercuse infectoria galls Colorectal cancer Apoptosis Autophagy Reactive oxygen species |
url |
https://doi.org/10.1186/s12906-020-03167-0 |
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