Molecular Mapping of QTLs for Heat Tolerance in Chickpea
Chickpea (Cicer arietinum L.), a cool-season legume, is increasingly affected by heat-stress at reproductive stage due to changes in global climatic conditions and cropping systems. Identifying quantitative trait loci (QTLs) for heat tolerance may facilitate breeding for heat tolerant varieties. The...
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doaj-21a93097d45b43adae9806a6bb1926de2020-11-24T21:08:05ZengMDPI AGInternational Journal of Molecular Sciences1422-00672018-07-01198216610.3390/ijms19082166ijms19082166Molecular Mapping of QTLs for Heat Tolerance in ChickpeaPronob J. Paul0Srinivasan Samineni1Mahendar Thudi2Sobhan B. Sajja3Abhishek Rathore4Roma R. Das5Aamir W. Khan6Sushil K. Chaturvedi7Gera Roopa Lavanya8Rajeev. K. Varshney9Pooran M. Gaur10International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru Hyderabad 502324, IndiaInternational Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru Hyderabad 502324, IndiaInternational Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru Hyderabad 502324, IndiaInternational Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru Hyderabad 502324, IndiaInternational Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru Hyderabad 502324, IndiaInternational Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru Hyderabad 502324, IndiaInternational Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru Hyderabad 502324, IndiaICAR-Indian Institute of Pulses Research (ICAR-IIPR), Kanpur 208024, IndiaDepartment of Genetics and Plant Breeding, Sam Higginbottom University of Agriculture, Technology and Sciences (SHUATS), Allahabad 211007, IndiaInternational Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru Hyderabad 502324, IndiaInternational Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru Hyderabad 502324, IndiaChickpea (Cicer arietinum L.), a cool-season legume, is increasingly affected by heat-stress at reproductive stage due to changes in global climatic conditions and cropping systems. Identifying quantitative trait loci (QTLs) for heat tolerance may facilitate breeding for heat tolerant varieties. The present study was aimed at identifying QTLs associated with heat tolerance in chickpea using 292 F8-9 recombinant inbred lines (RILs) developed from the cross ICC 4567 (heat sensitive) × ICC 15614 (heat tolerant). Phenotyping of RILs was undertaken for two heat-stress (late sown) and one non-stress (normal sown) environments. A genetic map spanning 529.11 cM and comprising 271 genotyping by sequencing (GBS) based single nucleotide polymorphism (SNP) markers was constructed. Composite interval mapping (CIM) analysis revealed two consistent genomic regions harbouring four QTLs each on CaLG05 and CaLG06. Four major QTLs for number of filled pods per plot (FPod), total number of seeds per plot (TS), grain yield per plot (GY) and % pod setting (%PodSet), located in the CaLG05 genomic region, were found to have cumulative phenotypic variation of above 50%. Nineteen pairs of epistatic QTLs showed significant epistatic effect, and non-significant QTL × environment interaction effect, except for harvest index (HI) and biomass (BM). A total of 25 putative candidate genes for heat-stress were identified in the two major genomic regions. This is the first report on QTLs for heat-stress response in chickpea. The markers linked to the above mentioned four major QTLs can facilitate marker-assisted breeding for heat tolerance in chickpea.http://www.mdpi.com/1422-0067/19/8/2166abiotic stressCicer arietinumcandidate genesgeneticsheat-stressmolecular breeding |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pronob J. Paul Srinivasan Samineni Mahendar Thudi Sobhan B. Sajja Abhishek Rathore Roma R. Das Aamir W. Khan Sushil K. Chaturvedi Gera Roopa Lavanya Rajeev. K. Varshney Pooran M. Gaur |
spellingShingle |
Pronob J. Paul Srinivasan Samineni Mahendar Thudi Sobhan B. Sajja Abhishek Rathore Roma R. Das Aamir W. Khan Sushil K. Chaturvedi Gera Roopa Lavanya Rajeev. K. Varshney Pooran M. Gaur Molecular Mapping of QTLs for Heat Tolerance in Chickpea International Journal of Molecular Sciences abiotic stress Cicer arietinum candidate genes genetics heat-stress molecular breeding |
author_facet |
Pronob J. Paul Srinivasan Samineni Mahendar Thudi Sobhan B. Sajja Abhishek Rathore Roma R. Das Aamir W. Khan Sushil K. Chaturvedi Gera Roopa Lavanya Rajeev. K. Varshney Pooran M. Gaur |
author_sort |
Pronob J. Paul |
title |
Molecular Mapping of QTLs for Heat Tolerance in Chickpea |
title_short |
Molecular Mapping of QTLs for Heat Tolerance in Chickpea |
title_full |
Molecular Mapping of QTLs for Heat Tolerance in Chickpea |
title_fullStr |
Molecular Mapping of QTLs for Heat Tolerance in Chickpea |
title_full_unstemmed |
Molecular Mapping of QTLs for Heat Tolerance in Chickpea |
title_sort |
molecular mapping of qtls for heat tolerance in chickpea |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2018-07-01 |
description |
Chickpea (Cicer arietinum L.), a cool-season legume, is increasingly affected by heat-stress at reproductive stage due to changes in global climatic conditions and cropping systems. Identifying quantitative trait loci (QTLs) for heat tolerance may facilitate breeding for heat tolerant varieties. The present study was aimed at identifying QTLs associated with heat tolerance in chickpea using 292 F8-9 recombinant inbred lines (RILs) developed from the cross ICC 4567 (heat sensitive) × ICC 15614 (heat tolerant). Phenotyping of RILs was undertaken for two heat-stress (late sown) and one non-stress (normal sown) environments. A genetic map spanning 529.11 cM and comprising 271 genotyping by sequencing (GBS) based single nucleotide polymorphism (SNP) markers was constructed. Composite interval mapping (CIM) analysis revealed two consistent genomic regions harbouring four QTLs each on CaLG05 and CaLG06. Four major QTLs for number of filled pods per plot (FPod), total number of seeds per plot (TS), grain yield per plot (GY) and % pod setting (%PodSet), located in the CaLG05 genomic region, were found to have cumulative phenotypic variation of above 50%. Nineteen pairs of epistatic QTLs showed significant epistatic effect, and non-significant QTL × environment interaction effect, except for harvest index (HI) and biomass (BM). A total of 25 putative candidate genes for heat-stress were identified in the two major genomic regions. This is the first report on QTLs for heat-stress response in chickpea. The markers linked to the above mentioned four major QTLs can facilitate marker-assisted breeding for heat tolerance in chickpea. |
topic |
abiotic stress Cicer arietinum candidate genes genetics heat-stress molecular breeding |
url |
http://www.mdpi.com/1422-0067/19/8/2166 |
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