Electrophoretic Mobility of Cardiac Myosin Heavy Chain Isoforms Revisited: Application of MALDI TOF/TOF Analysis
The expression of two cardiac myosin heavy chain (MyHC) isoforms in response to the thyroid status was studied in left ventricles (LVs) of Lewis rats. Major MyHC isoform in euthyroid and hyperthyroid LVs had a higher mobility on SDS-PAGE, whereas hypothyroid LVs predominantly contained a MyHC isofor...
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doaj-21ab2255ebaf46b58080f5947e2a619b2020-11-25T01:38:42ZengHindawi LimitedJournal of Biomedicine and Biotechnology1110-72431110-72512011-01-01201110.1155/2011/634253634253Electrophoretic Mobility of Cardiac Myosin Heavy Chain Isoforms Revisited: Application of MALDI TOF/TOF AnalysisPetra Arnostova0Petr L. Jedelsky1Tomáš Soukup2Jitka Zurmanova3Department of Physiology, Faculty of Science, Charles University in Prague, 12843 Prague, Czech RepublicDepartment of Cell Biology, Faculty of Science, Charles University in Prague, 12843 Prague, Czech RepublicInstitute of Physiology, Academy of Sciences of the Czech Republic, v.v.i., 14220 Prague, Czech RepublicDepartment of Physiology, Faculty of Science, Charles University in Prague, 12843 Prague, Czech RepublicThe expression of two cardiac myosin heavy chain (MyHC) isoforms in response to the thyroid status was studied in left ventricles (LVs) of Lewis rats. Major MyHC isoform in euthyroid and hyperthyroid LVs had a higher mobility on SDS-PAGE, whereas hypothyroid LVs predominantly contained a MyHC isoform with a lower mobility corresponding to that of the control soleus muscle. By comparing the MyHC profiles obtained under altered thyroid states together with the control soleus, we concluded that MyHCα was represented by the lower band with higher mobility and MyHCβ by the upper band. The identity of these two bands in SDS-PAGE gels was confirmed by western blot and mass spectrometry. Thus, in contrast to the literature data, we found that the MyHCα possessed a higher mobility rate than the MyHCβ isoform. Our data highlighted the importance of the careful identification of the MyHCα and MyHCβ isoforms analyzed by the SDS-PAGE.http://dx.doi.org/10.1155/2011/634253 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Petra Arnostova Petr L. Jedelsky Tomáš Soukup Jitka Zurmanova |
spellingShingle |
Petra Arnostova Petr L. Jedelsky Tomáš Soukup Jitka Zurmanova Electrophoretic Mobility of Cardiac Myosin Heavy Chain Isoforms Revisited: Application of MALDI TOF/TOF Analysis Journal of Biomedicine and Biotechnology |
author_facet |
Petra Arnostova Petr L. Jedelsky Tomáš Soukup Jitka Zurmanova |
author_sort |
Petra Arnostova |
title |
Electrophoretic Mobility of Cardiac Myosin Heavy Chain Isoforms Revisited: Application of MALDI TOF/TOF Analysis |
title_short |
Electrophoretic Mobility of Cardiac Myosin Heavy Chain Isoforms Revisited: Application of MALDI TOF/TOF Analysis |
title_full |
Electrophoretic Mobility of Cardiac Myosin Heavy Chain Isoforms Revisited: Application of MALDI TOF/TOF Analysis |
title_fullStr |
Electrophoretic Mobility of Cardiac Myosin Heavy Chain Isoforms Revisited: Application of MALDI TOF/TOF Analysis |
title_full_unstemmed |
Electrophoretic Mobility of Cardiac Myosin Heavy Chain Isoforms Revisited: Application of MALDI TOF/TOF Analysis |
title_sort |
electrophoretic mobility of cardiac myosin heavy chain isoforms revisited: application of maldi tof/tof analysis |
publisher |
Hindawi Limited |
series |
Journal of Biomedicine and Biotechnology |
issn |
1110-7243 1110-7251 |
publishDate |
2011-01-01 |
description |
The expression of two cardiac myosin heavy chain (MyHC) isoforms in response to the thyroid status was studied in left ventricles (LVs) of Lewis rats. Major MyHC isoform in euthyroid and hyperthyroid LVs had a higher mobility on SDS-PAGE, whereas hypothyroid LVs predominantly contained a MyHC isoform with a lower mobility corresponding to that of the control soleus muscle. By comparing the MyHC profiles obtained under altered thyroid states together with the control soleus, we concluded that MyHCα was represented by the lower band with higher mobility and MyHCβ by the upper band. The identity of these two bands in SDS-PAGE gels was confirmed by western blot and mass spectrometry. Thus, in contrast to the literature data, we found that the MyHCα possessed a higher mobility rate than the MyHCβ isoform. Our data highlighted the importance of the careful identification of the MyHCα and MyHCβ isoforms analyzed by the SDS-PAGE. |
url |
http://dx.doi.org/10.1155/2011/634253 |
work_keys_str_mv |
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