Dental Biofilm and Laboratory Microbial Culture Models for Cariology Research
Dental caries form through a complex interaction over time among dental plaque, fermentable carbohydrate, and host factors (including teeth and saliva). As a key factor, dental plaque or biofilm substantially influence the characteristic of the carious lesions. Laboratory microbial culture models ar...
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doaj-21d98b5542454ac5ab1964bda08562052020-11-25T01:36:02ZengMDPI AGDentistry Journal2304-67672017-06-01522110.3390/dj5020021dj5020021Dental Biofilm and Laboratory Microbial Culture Models for Cariology ResearchOllie Yiru Yu0Irene Shuping Zhao1May Lei Mei2Edward Chin-Man Lo3Chun-Hung Chu4Faculty of Dentistry, The University of Hong Kong, Hong Kong, ChinaFaculty of Dentistry, The University of Hong Kong, Hong Kong, ChinaFaculty of Dentistry, The University of Hong Kong, Hong Kong, ChinaFaculty of Dentistry, The University of Hong Kong, Hong Kong, ChinaFaculty of Dentistry, The University of Hong Kong, Hong Kong, ChinaDental caries form through a complex interaction over time among dental plaque, fermentable carbohydrate, and host factors (including teeth and saliva). As a key factor, dental plaque or biofilm substantially influence the characteristic of the carious lesions. Laboratory microbial culture models are often used because they provide a controllable and constant environment for cariology research. Moreover, they do not have ethical problems associated with clinical studies. The design of the microbial culture model varies from simple to sophisticated according to the purpose of the investigation. Each model is a compromise between the reality of the oral cavity and the simplification of the model. Researchers, however, can still obtain meaningful and useful results from the models they select. Laboratory microbial culture models can be categorized into a closed system and an open system. Models in the closed system have a finite supply of nutrients, and are also simple and cost-effective. Models in the open system enabled the supply of a fresh culture medium and the removal of metabolites and spent culture liquid simultaneously. They provide better regulation of the biofilm growth rate than the models in the closed system. This review paper gives an overview of the dental plaque biofilm and laboratory microbial culture models used for cariology research.http://www.mdpi.com/2304-6767/5/2/21biofilmdental plaquedemineralizationremineralizationcariesreview |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ollie Yiru Yu Irene Shuping Zhao May Lei Mei Edward Chin-Man Lo Chun-Hung Chu |
spellingShingle |
Ollie Yiru Yu Irene Shuping Zhao May Lei Mei Edward Chin-Man Lo Chun-Hung Chu Dental Biofilm and Laboratory Microbial Culture Models for Cariology Research Dentistry Journal biofilm dental plaque demineralization remineralization caries review |
author_facet |
Ollie Yiru Yu Irene Shuping Zhao May Lei Mei Edward Chin-Man Lo Chun-Hung Chu |
author_sort |
Ollie Yiru Yu |
title |
Dental Biofilm and Laboratory Microbial Culture Models for Cariology Research |
title_short |
Dental Biofilm and Laboratory Microbial Culture Models for Cariology Research |
title_full |
Dental Biofilm and Laboratory Microbial Culture Models for Cariology Research |
title_fullStr |
Dental Biofilm and Laboratory Microbial Culture Models for Cariology Research |
title_full_unstemmed |
Dental Biofilm and Laboratory Microbial Culture Models for Cariology Research |
title_sort |
dental biofilm and laboratory microbial culture models for cariology research |
publisher |
MDPI AG |
series |
Dentistry Journal |
issn |
2304-6767 |
publishDate |
2017-06-01 |
description |
Dental caries form through a complex interaction over time among dental plaque, fermentable carbohydrate, and host factors (including teeth and saliva). As a key factor, dental plaque or biofilm substantially influence the characteristic of the carious lesions. Laboratory microbial culture models are often used because they provide a controllable and constant environment for cariology research. Moreover, they do not have ethical problems associated with clinical studies. The design of the microbial culture model varies from simple to sophisticated according to the purpose of the investigation. Each model is a compromise between the reality of the oral cavity and the simplification of the model. Researchers, however, can still obtain meaningful and useful results from the models they select. Laboratory microbial culture models can be categorized into a closed system and an open system. Models in the closed system have a finite supply of nutrients, and are also simple and cost-effective. Models in the open system enabled the supply of a fresh culture medium and the removal of metabolites and spent culture liquid simultaneously. They provide better regulation of the biofilm growth rate than the models in the closed system. This review paper gives an overview of the dental plaque biofilm and laboratory microbial culture models used for cariology research. |
topic |
biofilm dental plaque demineralization remineralization caries review |
url |
http://www.mdpi.com/2304-6767/5/2/21 |
work_keys_str_mv |
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