Mechanism of Xuebijing injection on hepatic ischemia-reperfusion injury based on network pharmacology
Objective: Using network pharmacology to predict the main active ingredients, targets and signaling pathways of Xuebijing injection in the treatment of hepatic ischemia-reperfusion injury and explore its potential mechanism of action. Methods: Screen the active ingredients and their targets of Da...
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doaj-21e5755080b345eb8fb7f961444622772021-03-04T08:16:17ZengEditorial Board of Journal of Hainan Medical UniversityJournal of Hainan Medical University1007-12371007-12372021-02-012734451Mechanism of Xuebijing injection on hepatic ischemia-reperfusion injury based on network pharmacologyYuan-Yuan Ding0Xuan Wang1Affiliated 81st Hospital's of Nanjing University of Chinese Medicine, Nanjing 210002, ChinaDepartment of General Surgery, General Hospital of Eastern Theater Command, PLA, Nanjing 210002, ChinaObjective: Using network pharmacology to predict the main active ingredients, targets and signaling pathways of Xuebijing injection in the treatment of hepatic ischemia-reperfusion injury and explore its potential mechanism of action. Methods: Screen the active ingredients and their targets of Danshen, Honghua, Chishao, Chuanxiong, and Danggui in Xuebijing injection through Traditional Chinese Medicine Systems Pharmacology (TCMSP) database and the Hepatic ischemia-reperfusion injury related targets through Online Mendelian Inheritance in Man (OMIM) and GeneCards Suite (The Human Gene Database) database. And acquire drug-disease intersection targets at the same time.The STRING database was used to construct a protein-protein interaction (PPI) network and topologically screen the central targets. Use the R language online search Bioconductor platform to perform GO function enrichment on the target; Database for Annotation, Visualization and Integrated Discovery (DAVID) database was used to perform KEGG channel enrichment analysis on the target. Use Cytoscape 3.7.2 to construct a " ingredient-target-pathway " network diagram and perform a topology analysis. Results: A total of 115 active ingredients were selected from Xuebijing injection, including Quercetin, Luteolin, Kaempferol, Beta-carotene, and Tanshinone IIa ,etc. It corresponds to 217 targets. There are 1057 disease-related targets, and 114 drug-disease common targets. PPI topologically screened out 17 target proteins. Topological analysis of the network graph obtained 15 target genes. Thire intersection contains key targets such as JUN, PPARG, PTGS2, AKT1 and MAPK1. A total of 137 related signaling pathways were obtained by GO enrichment analysis. A total of 8 signaling pathways were obtained through KEGG enrichment (P <0.05, FDR <0.05), among which signaling pathways such as Toll-like receptors, T cell receptors, NOD-like receptors, VEGF, and ErbB played an important role in immune regulation, anti-apoptosis, anti-inflammatory, anti-oxidation, and promoting angiogenesis in the treatment. Conclusion: Xuebijing injection can treat hepatic ischemia-reperfusion injury through multiple components, multiple targets and multiple pathways.http://www.hnykdxxb.com/PDF/202103/09.pdfnetwork pharmacologyxuebijing injectionhepatic ischemia-reperfusion injurymechanism of action |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yuan-Yuan Ding Xuan Wang |
spellingShingle |
Yuan-Yuan Ding Xuan Wang Mechanism of Xuebijing injection on hepatic ischemia-reperfusion injury based on network pharmacology Journal of Hainan Medical University network pharmacology xuebijing injection hepatic ischemia-reperfusion injury mechanism of action |
author_facet |
Yuan-Yuan Ding Xuan Wang |
author_sort |
Yuan-Yuan Ding |
title |
Mechanism of Xuebijing injection on hepatic ischemia-reperfusion injury based on network pharmacology |
title_short |
Mechanism of Xuebijing injection on hepatic ischemia-reperfusion injury based on network pharmacology |
title_full |
Mechanism of Xuebijing injection on hepatic ischemia-reperfusion injury based on network pharmacology |
title_fullStr |
Mechanism of Xuebijing injection on hepatic ischemia-reperfusion injury based on network pharmacology |
title_full_unstemmed |
Mechanism of Xuebijing injection on hepatic ischemia-reperfusion injury based on network pharmacology |
title_sort |
mechanism of xuebijing injection on hepatic ischemia-reperfusion injury based on network pharmacology |
publisher |
Editorial Board of Journal of Hainan Medical University |
series |
Journal of Hainan Medical University |
issn |
1007-1237 1007-1237 |
publishDate |
2021-02-01 |
description |
Objective: Using network pharmacology to predict the main active ingredients, targets and
signaling pathways of Xuebijing injection in the treatment of hepatic ischemia-reperfusion
injury and explore its potential mechanism of action. Methods: Screen the active ingredients
and their targets of Danshen, Honghua, Chishao, Chuanxiong, and Danggui in Xuebijing
injection through Traditional Chinese Medicine Systems Pharmacology (TCMSP) database and
the Hepatic ischemia-reperfusion injury related targets through Online Mendelian Inheritance
in Man (OMIM) and GeneCards Suite (The Human Gene Database) database. And acquire
drug-disease intersection targets at the same time.The STRING database was used to construct
a protein-protein interaction (PPI) network and topologically screen the central targets. Use
the R language online search Bioconductor platform to perform GO function enrichment on
the target; Database for Annotation, Visualization and Integrated Discovery (DAVID) database
was used to perform KEGG channel enrichment analysis on the target. Use Cytoscape 3.7.2
to construct a " ingredient-target-pathway " network diagram and perform a topology analysis.
Results: A total of 115 active ingredients were selected from Xuebijing injection, including
Quercetin, Luteolin, Kaempferol, Beta-carotene, and Tanshinone IIa ,etc. It corresponds to
217 targets. There are 1057 disease-related targets, and 114 drug-disease common targets.
PPI topologically screened out 17 target proteins. Topological analysis of the network graph
obtained 15 target genes. Thire intersection contains key targets such as JUN, PPARG,
PTGS2, AKT1 and MAPK1. A total of 137 related signaling pathways were obtained by GO
enrichment analysis. A total of 8 signaling pathways were obtained through KEGG enrichment
(P <0.05, FDR <0.05), among which signaling pathways such as Toll-like receptors, T
cell receptors, NOD-like receptors, VEGF, and ErbB played an important role in immune
regulation, anti-apoptosis, anti-inflammatory, anti-oxidation, and promoting angiogenesis in
the treatment. Conclusion: Xuebijing injection can treat hepatic ischemia-reperfusion injury
through multiple components, multiple targets and multiple pathways. |
topic |
network pharmacology xuebijing injection hepatic ischemia-reperfusion injury mechanism of action |
url |
http://www.hnykdxxb.com/PDF/202103/09.pdf |
work_keys_str_mv |
AT yuanyuanding mechanismofxuebijinginjectiononhepaticischemiareperfusioninjurybasedonnetworkpharmacology AT xuanwang mechanismofxuebijinginjectiononhepaticischemiareperfusioninjurybasedonnetworkpharmacology |
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1724232108412502016 |