Identification of molecular markers for type 2 Diabetes mellitus in Sidoarjo, Indonesia

Identification of molecular markers for type 2 Diabetes mellitus in Sidoarjo, Indonesia

T2DM can be triggered by two collaborating factors, namely genetics and the environment. This study aimed to identify genetic markers that can be used to detect the possibility of a person having T2D using the random amplified polymorphism DNA (RAPD) method. The study was carried out cross-sectional...

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Bibliographic Details
Main Authors: Miftahul Mushlih, Fitri Kumala Sari, Hindah Sabrina Amin, Siti Asriani Iknan
Format: Article
Language:Indonesian
Published: Poltekkes Kemenkes Yogyakarta 2020-12-01
Series:Jurnal Teknologi Laboratorium
Subjects:
diabetes mellitus
genetic disorder
pcr-rapd
polymorphism
molecular marker
Online Access:https://teknolabjournal.com/index.php/Jtl/article/view/235
Description
Summary:T2DM can be triggered by two collaborating factors, namely genetics and the environment. This study aimed to identify genetic markers that can be used to detect the possibility of a person having T2D using the random amplified polymorphism DNA (RAPD) method. The study was carried out cross-sectional and involved 60 samples consisting of 30 positive T2D samples and 30 negative samples T2D. The primer used for PCR-RAPD was D20 (5'-ACCCGGTCAC-3’). The PCR-RAPD results were then analyzed using the scoring method and analyzed using the non-parametric Chi-Square test (cl: 95%). Among T2D, 576 bp band were confirmed to be markers in the patients.
ISSN:2338-5634
2580-0191

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