Identification of molecular markers for type 2 Diabetes mellitus in Sidoarjo, Indonesia

• Main Authors: Miftahul Mushlih, Fitri Kumala Sari, Hindah Sabrina Amin, Siti Asriani Iknan
• Format: Article
• Language: Indonesian
• Published: Poltekkes Kemenkes Yogyakarta 2020-12-01
• Series: Jurnal Teknologi Laboratorium
• Subjects: diabetes mellitus; genetic disorder; pcr-rapd; polymorphism; molecular marker
• Online Access: https://teknolabjournal.com/index.php/Jtl/article/view/235

T2DM can be triggered by two collaborating factors, namely genetics and the environment. This study aimed to identify genetic markers that can be used to detect the possibility of a person having T2D using the random amplified polymorphism DNA (RAPD) method. The study was carried out cross-sectional and involved 60 samples consisting of 30 positive T2D samples and 30 negative samples T2D. The primer used for PCR-RAPD was D20 (5'-ACCCGGTCAC-3’). The PCR-RAPD results were then analyzed using the scoring method and analyzed using the non-parametric Chi-Square test (cl: 95%). Among T2D, 576 bp band were confirmed to be markers in the patients.