Plasma glutathione and oxidized glutathione level, glutathione/oxidized glutathione ratio, and albumin concentration in complicated and uncomplicated falciparum malaria

• Main Authors: Loeki Enggar Fitri, Agustin Iskandar, Teguh Wahju Sardjono, Ummu Ditya Erliana, Widya Rahmawati, Didi Candradikusuma, Utama Budi Saputra, Eko Suhartono, Bambang Setiawan, Erma Sulistyaningsih
• Format: Article
• Language: English
• Published: Wolters Kluwer Medknow Publications 2016-08-01
• Series: Asian Pacific Journal of Tropical Biomedicine
• Subjects: Albumin; Complicated malaria; Glutathione; Oxidized glutathione; Glutathione/oxidized glutathione ratio; Uncomplicated malaria
• Online Access: http://www.sciencedirect.com/science/article/pii/S2221169115308911

Objective: To compare the level of glutathione (GSH) and oxidized glutathione (GSSG), the ratio of GSH/GSSG and the concentration of albumin in plasma of patients with complicated and un-complicated falciparum malaria. Methods: This research was a cross sectional study using comparison analysis with the plasma GSH and GSSG, the ratio of plasma GSH/GSSG and the concentration of plasma albumin as variables. The complicated malaria patients were obtained from Dr. Saiful Anwar Hospital Malang, whereas uncomplicated malaria patients were obtained from the Regency of Pleihari South Kalimantan. Plasma GSH and GSSG levels were determined by the spectrophotometer at the wave length of 412 nm, whereas the concentration of albumin was determined by bromocresol green method in the pH of 4.1. Results: There were no significant differences between the level of plasma GSH and GSSG in complicated and uncomplicated malaria patients, as well as the ratio of plasma GSH/GSSG in the two groups (P = 0.373; P = 0.538; and P = 0.615, respectively, independent t-test). In contrast, the plasma albumin concentration in complicated malaria patients were significantly higher than uncomplicated malaria patients (P = 0.000, Mann Whitney U test). Conclusions: It can be concluded that the average of plasma GSH and GSSG level, also plasma GSH/GSSG ratio in complicated malaria are not different from uncomplicated malaria. Although plasma concentration of albumin in both groups is below the normal range, there is an increase in complicated malaria that might be as compensation of oxidative stress.