Neuronal Differentiation of NT2 Cells in Monolayer and Spheroid Cultures
Metabolism and differentiation of cultured cells are influenced by changes in cellular morphology. In this study, we investigated the differences in cell proliferation and neuronal differentiation of NT2 cells in monolayer (2D) and spheroid (3D) cultures. In the monolayer culture, the cells adhered...
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EDP Sciences
2021-01-01
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Online Access: | https://www.matec-conferences.org/articles/matecconf/pdf/2021/02/matecconf_apcche21_07008.pdf |
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doaj-220d77f234f04fc7991c73fc6a7483bd2021-01-15T10:19:30ZengEDP SciencesMATEC Web of Conferences2261-236X2021-01-013330700810.1051/matecconf/202133307008matecconf_apcche21_07008Neuronal Differentiation of NT2 Cells in Monolayer and Spheroid CulturesKitano OtomeNakazawa KohjiMetabolism and differentiation of cultured cells are influenced by changes in cellular morphology. In this study, we investigated the differences in cell proliferation and neuronal differentiation of NT2 cells in monolayer (2D) and spheroid (3D) cultures. In the monolayer culture, the cells adhered and extended on a tissue culture plate. For the spheroid culture, we fabricated a microwell chip comprising 195 circular microwells (600 ìm in diameter) on a cutture plate, and the surface was modified with polyethylene glycol to promote spheroid formation. The cells were aggregated in each microwell and formed spheroids within 24 hours of culture, and the spheroid morphology was maintained thoughout the culture period. Although the cell proliferation ability in monolayer culture was higher than that in spheroid culture, the neuronal differentiation ability of NT2 spheroid culture was higher than that in monolayer culture. Furthermore, the neuronal differentiation of NT2 spheroids was dramatically enhanced by retinoic acid treatment. These results indicate that NT2 cell properties are influenced by differences in cell morphologies, and that spheroid culture is a promising technique to induce neuronal differentiation.https://www.matec-conferences.org/articles/matecconf/pdf/2021/02/matecconf_apcche21_07008.pdf |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kitano Otome Nakazawa Kohji |
spellingShingle |
Kitano Otome Nakazawa Kohji Neuronal Differentiation of NT2 Cells in Monolayer and Spheroid Cultures MATEC Web of Conferences |
author_facet |
Kitano Otome Nakazawa Kohji |
author_sort |
Kitano Otome |
title |
Neuronal Differentiation of NT2 Cells in Monolayer and Spheroid Cultures |
title_short |
Neuronal Differentiation of NT2 Cells in Monolayer and Spheroid Cultures |
title_full |
Neuronal Differentiation of NT2 Cells in Monolayer and Spheroid Cultures |
title_fullStr |
Neuronal Differentiation of NT2 Cells in Monolayer and Spheroid Cultures |
title_full_unstemmed |
Neuronal Differentiation of NT2 Cells in Monolayer and Spheroid Cultures |
title_sort |
neuronal differentiation of nt2 cells in monolayer and spheroid cultures |
publisher |
EDP Sciences |
series |
MATEC Web of Conferences |
issn |
2261-236X |
publishDate |
2021-01-01 |
description |
Metabolism and differentiation of cultured cells are influenced by changes in cellular morphology. In this study, we investigated the differences in cell proliferation and neuronal differentiation of NT2 cells in monolayer (2D) and spheroid (3D) cultures. In the monolayer culture, the cells adhered and extended on a tissue culture plate. For the spheroid culture, we fabricated a microwell chip comprising 195 circular microwells (600 ìm in diameter) on a cutture plate, and the surface was modified with polyethylene glycol to promote spheroid formation. The cells were aggregated in each microwell and formed spheroids within 24 hours of culture, and the spheroid morphology was maintained thoughout the culture period. Although the cell proliferation ability in monolayer culture was higher than that in spheroid culture, the neuronal differentiation ability of NT2 spheroid culture was higher than that in monolayer culture. Furthermore, the neuronal differentiation of NT2 spheroids was dramatically enhanced by retinoic acid treatment. These results indicate that NT2 cell properties are influenced by differences in cell morphologies, and that spheroid culture is a promising technique to induce neuronal differentiation. |
url |
https://www.matec-conferences.org/articles/matecconf/pdf/2021/02/matecconf_apcche21_07008.pdf |
work_keys_str_mv |
AT kitanootome neuronaldifferentiationofnt2cellsinmonolayerandspheroidcultures AT nakazawakohji neuronaldifferentiationofnt2cellsinmonolayerandspheroidcultures |
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