MADS-Box Transcription Factor MadsA Regulates Dimorphic Transition, Conidiation, and Germination of Talaromyces marneffei
The opportunistic human pathogen Talaromyces marneffei exhibits a temperature-dependent dimorphic transition, which is closely related with its pathogenicity. This species grows as multinucleate mycelia that produce infectious conidia at 25°C, while undergoes a dimorphic transition to generate uninu...
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Frontiers Media S.A.
2018-08-01
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Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.01781/full |
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Article |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qiangyi Wang Minghao Du Minghao Du Shuai Wang Linxia Liu Linxia Liu Liming Xiao Liming Xiao Linqi Wang Tong Li Hui Zhuang Ence Yang Ence Yang |
spellingShingle |
Qiangyi Wang Minghao Du Minghao Du Shuai Wang Linxia Liu Linxia Liu Liming Xiao Liming Xiao Linqi Wang Tong Li Hui Zhuang Ence Yang Ence Yang MADS-Box Transcription Factor MadsA Regulates Dimorphic Transition, Conidiation, and Germination of Talaromyces marneffei Frontiers in Microbiology Talaromyces marneffei madsA dimorphic transition transcriptome yeast-to-mycelium oxidative stress |
author_facet |
Qiangyi Wang Minghao Du Minghao Du Shuai Wang Linxia Liu Linxia Liu Liming Xiao Liming Xiao Linqi Wang Tong Li Hui Zhuang Ence Yang Ence Yang |
author_sort |
Qiangyi Wang |
title |
MADS-Box Transcription Factor MadsA Regulates Dimorphic Transition, Conidiation, and Germination of Talaromyces marneffei |
title_short |
MADS-Box Transcription Factor MadsA Regulates Dimorphic Transition, Conidiation, and Germination of Talaromyces marneffei |
title_full |
MADS-Box Transcription Factor MadsA Regulates Dimorphic Transition, Conidiation, and Germination of Talaromyces marneffei |
title_fullStr |
MADS-Box Transcription Factor MadsA Regulates Dimorphic Transition, Conidiation, and Germination of Talaromyces marneffei |
title_full_unstemmed |
MADS-Box Transcription Factor MadsA Regulates Dimorphic Transition, Conidiation, and Germination of Talaromyces marneffei |
title_sort |
mads-box transcription factor madsa regulates dimorphic transition, conidiation, and germination of talaromyces marneffei |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2018-08-01 |
description |
The opportunistic human pathogen Talaromyces marneffei exhibits a temperature-dependent dimorphic transition, which is closely related with its pathogenicity. This species grows as multinucleate mycelia that produce infectious conidia at 25°C, while undergoes a dimorphic transition to generate uninucleate yeast form cells at 37°C. The mechanisms of phenotype switching are not fully understood. The transcription factor madsA gene is a member of the MADS-box gene family. Previously, it was found that overexpression of madsA gene resulted in mycelial growth instead of yeast form at 37°C. In the current study, the madsA deletion mutant (ΔmadsA) and complemented strain (CMA) were constructed by genetic manipulation. We compared the phenotypes, growth, conidiation, conidial germination and susceptibility to stresses (including osmotic and oxidative) of the ΔmadsA with the wild-type (WT) and CMA strains. The results showed that the ΔmadsA displayed a faster process of the yeast-to-mycelium transition than the WT and CMA. In addition, the deletion of madsA led to a delay in conidia production and conidial germination. The tolerance of ΔmadsA conidia to hydrogen peroxide was better than that of the WT and CMA strains. Then, RNA-seq was performed to identify differences in gene expression between the ΔmadsA mutant and WT strain during the yeast phase, mycelium phase, yeast-to-mycelium transition and mycelium-to-yeast transition, respectively. Gene ontology functional enrichment analyses indicated that some important processes such as transmembrane transport, oxidation-reduction process, protein catabolic process and response to oxidative stress were affected by the madsA deletion. Together, our results suggest that madsA functions as a global regulator involved in the conidiation and germination, especially in the dimorphic transition of T. marneffei. Its roles in the survival, pathogenicity and transmission of T. marneffei require further investigation. |
topic |
Talaromyces marneffei madsA dimorphic transition transcriptome yeast-to-mycelium oxidative stress |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2018.01781/full |
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doaj-2216aeb470ac4324a8aacfede99b42452020-11-24T23:13:10ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-08-01910.3389/fmicb.2018.01781360473MADS-Box Transcription Factor MadsA Regulates Dimorphic Transition, Conidiation, and Germination of Talaromyces marneffeiQiangyi Wang0Minghao Du1Minghao Du2Shuai Wang3Linxia Liu4Linxia Liu5Liming Xiao6Liming Xiao7Linqi Wang8Tong Li9Hui Zhuang10Ence Yang11Ence Yang12Department of Microbiology & Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, ChinaDepartment of Microbiology & Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, ChinaInstitute of Systems Biomedicine, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, ChinaDepartment of Laboratorial Science and Technology, School of Public Health, Peking University, Beijing, ChinaState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaInstitute of Microbiology, University of Chinese Academy of Sciences, Beijing, ChinaDepartment of Microbiology & Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, ChinaInstitute of Systems Biomedicine, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, ChinaState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaDepartment of Microbiology & Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, ChinaDepartment of Microbiology & Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, ChinaDepartment of Microbiology & Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, ChinaInstitute of Systems Biomedicine, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, ChinaThe opportunistic human pathogen Talaromyces marneffei exhibits a temperature-dependent dimorphic transition, which is closely related with its pathogenicity. This species grows as multinucleate mycelia that produce infectious conidia at 25°C, while undergoes a dimorphic transition to generate uninucleate yeast form cells at 37°C. The mechanisms of phenotype switching are not fully understood. The transcription factor madsA gene is a member of the MADS-box gene family. Previously, it was found that overexpression of madsA gene resulted in mycelial growth instead of yeast form at 37°C. In the current study, the madsA deletion mutant (ΔmadsA) and complemented strain (CMA) were constructed by genetic manipulation. We compared the phenotypes, growth, conidiation, conidial germination and susceptibility to stresses (including osmotic and oxidative) of the ΔmadsA with the wild-type (WT) and CMA strains. The results showed that the ΔmadsA displayed a faster process of the yeast-to-mycelium transition than the WT and CMA. In addition, the deletion of madsA led to a delay in conidia production and conidial germination. The tolerance of ΔmadsA conidia to hydrogen peroxide was better than that of the WT and CMA strains. Then, RNA-seq was performed to identify differences in gene expression between the ΔmadsA mutant and WT strain during the yeast phase, mycelium phase, yeast-to-mycelium transition and mycelium-to-yeast transition, respectively. Gene ontology functional enrichment analyses indicated that some important processes such as transmembrane transport, oxidation-reduction process, protein catabolic process and response to oxidative stress were affected by the madsA deletion. Together, our results suggest that madsA functions as a global regulator involved in the conidiation and germination, especially in the dimorphic transition of T. marneffei. Its roles in the survival, pathogenicity and transmission of T. marneffei require further investigation.https://www.frontiersin.org/article/10.3389/fmicb.2018.01781/fullTalaromyces marneffeimadsAdimorphic transitiontranscriptomeyeast-to-myceliumoxidative stress |