<i>Helicobacter pylori</i> Stress-Response: Definition of the HrcA Regulon
Bacteria respond to different environmental stresses by reprogramming the transcription of specific genes whose proper expression is critical for their survival. In this regard, the heat-shock response, a widespread protective mechanism, triggers a sudden increase in the cellular concentration of di...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2019-10-01
|
Series: | Microorganisms |
Subjects: | |
Online Access: | https://www.mdpi.com/2076-2607/7/10/436 |
id |
doaj-223c7fffdae14e44b3b55cf7c582bb02 |
---|---|
record_format |
Article |
spelling |
doaj-223c7fffdae14e44b3b55cf7c582bb022020-11-25T01:32:42ZengMDPI AGMicroorganisms2076-26072019-10-0171043610.3390/microorganisms7100436microorganisms7100436<i>Helicobacter pylori</i> Stress-Response: Definition of the HrcA RegulonDavide Roncarati0Eva Pinatel1Elisabetta Fiore2Clelia Peano3Stefany Loibman4Vincenzo Scarlato5Department of Pharmacy and Biotechnology (FaBiT), University of Bologna, 40126 Bologna, ItalyInstitute of Biomedical Technologies, National Research Council, 20090 Milan, ItalyDepartment of Pharmacy and Biotechnology (FaBiT), University of Bologna, 40126 Bologna, ItalyInstitute of Genetic and Biomedical Research, UoS Milan, National Research Council, Rozzano, 20089 Milan, ItalyDepartment of Pharmacy and Biotechnology (FaBiT), University of Bologna, 40126 Bologna, ItalyDepartment of Pharmacy and Biotechnology (FaBiT), University of Bologna, 40126 Bologna, ItalyBacteria respond to different environmental stresses by reprogramming the transcription of specific genes whose proper expression is critical for their survival. In this regard, the heat-shock response, a widespread protective mechanism, triggers a sudden increase in the cellular concentration of different proteins, including molecular chaperones and proteases, to preserve protein folding and maintain cellular homeostasis. In the medically important gastric pathogen <i>Helicobacter pylori</i> the regulation of the principal heat-shock genes is under the transcriptional control of two repressor proteins named HspR and HrcA. To define the HrcA regulon, we carried out whole transcriptome analysis through RNA-sequencing, comparing the transcriptome of the <i>H. pylori</i> G27 wild type strain to that of the isogenic <i>hrcA</i>-knockout strain. Overall, differential gene expression analysis outlined 49 genes to be deregulated upon <i>hrcA</i> gene inactivation. Interestingly, besides controlling the transcription of genes coding for molecular chaperones and stress-related mediators, HrcA is involved in regulating the expression of proteins whose function is linked to several cellular processes crucial for bacterial survival and virulence. These include cell motility, membrane transporters, Lipopolysaccharide modifiers and adhesins. The role of HrcA as a central regulator of <i>H. pylori</i> transcriptome, as well as its interconnections with the HspR regulon are here analyzed and discussed. As the HrcA protein acts as a pleiotropic regulator, influencing the expression of several stress-unrelated genes, it may be considered a promising target for the design of new antimicrobial strategies.https://www.mdpi.com/2076-2607/7/10/436heat-shock responsestress conditionshrca repressor<i>helicobacter pylori</i>rna-sequencingtranscriptome |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Davide Roncarati Eva Pinatel Elisabetta Fiore Clelia Peano Stefany Loibman Vincenzo Scarlato |
spellingShingle |
Davide Roncarati Eva Pinatel Elisabetta Fiore Clelia Peano Stefany Loibman Vincenzo Scarlato <i>Helicobacter pylori</i> Stress-Response: Definition of the HrcA Regulon Microorganisms heat-shock response stress conditions hrca repressor <i>helicobacter pylori</i> rna-sequencing transcriptome |
author_facet |
Davide Roncarati Eva Pinatel Elisabetta Fiore Clelia Peano Stefany Loibman Vincenzo Scarlato |
author_sort |
Davide Roncarati |
title |
<i>Helicobacter pylori</i> Stress-Response: Definition of the HrcA Regulon |
title_short |
<i>Helicobacter pylori</i> Stress-Response: Definition of the HrcA Regulon |
title_full |
<i>Helicobacter pylori</i> Stress-Response: Definition of the HrcA Regulon |
title_fullStr |
<i>Helicobacter pylori</i> Stress-Response: Definition of the HrcA Regulon |
title_full_unstemmed |
<i>Helicobacter pylori</i> Stress-Response: Definition of the HrcA Regulon |
title_sort |
<i>helicobacter pylori</i> stress-response: definition of the hrca regulon |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2019-10-01 |
description |
Bacteria respond to different environmental stresses by reprogramming the transcription of specific genes whose proper expression is critical for their survival. In this regard, the heat-shock response, a widespread protective mechanism, triggers a sudden increase in the cellular concentration of different proteins, including molecular chaperones and proteases, to preserve protein folding and maintain cellular homeostasis. In the medically important gastric pathogen <i>Helicobacter pylori</i> the regulation of the principal heat-shock genes is under the transcriptional control of two repressor proteins named HspR and HrcA. To define the HrcA regulon, we carried out whole transcriptome analysis through RNA-sequencing, comparing the transcriptome of the <i>H. pylori</i> G27 wild type strain to that of the isogenic <i>hrcA</i>-knockout strain. Overall, differential gene expression analysis outlined 49 genes to be deregulated upon <i>hrcA</i> gene inactivation. Interestingly, besides controlling the transcription of genes coding for molecular chaperones and stress-related mediators, HrcA is involved in regulating the expression of proteins whose function is linked to several cellular processes crucial for bacterial survival and virulence. These include cell motility, membrane transporters, Lipopolysaccharide modifiers and adhesins. The role of HrcA as a central regulator of <i>H. pylori</i> transcriptome, as well as its interconnections with the HspR regulon are here analyzed and discussed. As the HrcA protein acts as a pleiotropic regulator, influencing the expression of several stress-unrelated genes, it may be considered a promising target for the design of new antimicrobial strategies. |
topic |
heat-shock response stress conditions hrca repressor <i>helicobacter pylori</i> rna-sequencing transcriptome |
url |
https://www.mdpi.com/2076-2607/7/10/436 |
work_keys_str_mv |
AT davideroncarati ihelicobacterpyloriistressresponsedefinitionofthehrcaregulon AT evapinatel ihelicobacterpyloriistressresponsedefinitionofthehrcaregulon AT elisabettafiore ihelicobacterpyloriistressresponsedefinitionofthehrcaregulon AT cleliapeano ihelicobacterpyloriistressresponsedefinitionofthehrcaregulon AT stefanyloibman ihelicobacterpyloriistressresponsedefinitionofthehrcaregulon AT vincenzoscarlato ihelicobacterpyloriistressresponsedefinitionofthehrcaregulon |
_version_ |
1725080407410475008 |