Bacterial Diversity and Community Structure of a Municipal Solid Waste Landfill: A Source of Lignocellulolytic Potential

• Main Authors: Ogechukwu Bose Chukwuma, Mohd Rafatullah, Husnul Azan Tajarudin, Norli Ismail
• Format: Article
• Language: English
• Published: MDPI AG 2021-05-01
• Series: Life
• Subjects: bacteria; biodiversity; landfill; lignocellulose biomass; lignocellulolytic enzyme; lignocellulolytic bacteria
• Online Access: https://www.mdpi.com/2075-1729/11/6/493

Omics have given rise to research on sparsely studied microbial communities such as the landfill, lignocellulolytic microorganisms and enzymes. The bacterial diversity of Municipal Solid Waste sediments was determined using the illumina MiSeq system after DNA extraction and Polymerase chain reactions. Data analysis was used to determine the community’s richness, diversity, and correlation with environmental factors. Physicochemical studies revealed sites with mesophilic and thermophilic temperature ranges and a mixture of acidic and alkaline pH values. Temperature and moisture content showed the highest correlation with the bacteria community. The bacterial analysis of the community DNA revealed 357,030 effective sequences and 1891 operational taxonomic units (OTUs) assigned. Forty phyla were found, with the dominant phyla <i>Proteobacteria</i>, <i>Firmicutes</i>, <i>Actinobacteria</i>, and <i>Bacteroidota</i>, while <i>Aerococcus</i>, <i>Stenotrophomonas</i>, and <i>Sporosarcina</i> were the dominant species. PICRUSt provided insight on community’s metabolic function, which was narrowed down to search for lignocellulolytic enzymes’ function. Cellulase, xylanase, esterase, and peroxidase were gene functions inferred from the data. This article reports on the first phylogenetic analysis of the Pulau Burung landfill bacterial community. These results will help to improve the understanding of organisms dominant in the landfill and the corresponding enzymes that contribute to lignocellulose breakdown.