Cytotoxicological Investigation of the Essential Oil and the Extracts of Cotula cinerea and Salvia verbenaca from Morocco
The objective of this work was to investigate the cytotoxicological effect of the extracts (hexane, ethyl acetate, and n-butanol) of Cotula cinerea and Salvia verbenaca in addition to the essential oil of Cotula cinerea. These plants are widely used in the Moroccan traditional folk medicine. The cyt...
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doaj-22572a2fe9d042d09bb462b24d3e0d132020-11-25T00:30:26ZengHindawi LimitedBioMed Research International2314-61332314-61412018-01-01201810.1155/2018/71639617163961Cytotoxicological Investigation of the Essential Oil and the Extracts of Cotula cinerea and Salvia verbenaca from MoroccoFatima-Ezzahrae Guaouguaou0Mohamed Alien Ahl Bebaha1Khalid Taghzouti2Abdelhakim Bouyahya3Youssef Bakri4Nadia Dakka5Nour Eddine Es-Safi6Mohammed V University in Rabat, LPCMIO, Materials Science Center (MSC), Ecole Normale Supérieure, Rabat, MoroccoMohammed V University in Rabat, LPCMIO, Materials Science Center (MSC), Ecole Normale Supérieure, Rabat, MoroccoLaboratory of Animal Physiology, Faculty of Sciences, Mohammed V University in Rabat, Rabat, MoroccoMohammed V University of Rabat, Faculty of Sciences, Department of Biology, Genomic Center of Human Pathologies, Laboratory of Human Pathologies Biology, Rabat, MoroccoMohammed V University of Rabat, Faculty of Sciences, Department of Biology, Genomic Center of Human Pathologies, Laboratory of Human Pathologies Biology, Rabat, MoroccoMohammed V University of Rabat, Faculty of Sciences, Department of Biology, Genomic Center of Human Pathologies, Laboratory of Human Pathologies Biology, Rabat, MoroccoMohammed V University in Rabat, LPCMIO, Materials Science Center (MSC), Ecole Normale Supérieure, Rabat, MoroccoThe objective of this work was to investigate the cytotoxicological effect of the extracts (hexane, ethyl acetate, and n-butanol) of Cotula cinerea and Salvia verbenaca in addition to the essential oil of Cotula cinerea. These plants are widely used in the Moroccan traditional folk medicine. The cytotoxic effect was explored against two cancer cell lines, Vero and RD, using the colorimetric MTT assay. The obtained results showed that the cytotoxicity differed according to the used extract with an efficient effect of Cotula cinerea extracts compared to Salvia verbenaca. A potent cytotoxicity was thus observed for the Cotula cinerea hexane extract which inhibited the growth of RD cell line at the lowest IC50 value (57.21±3.43 µg/mL). This was followed by the ethyl acetate extract and the essential oil with moderate effects against RD cell line and showed IC50 values of 187.52±6.27 µg/mL and 173.05±4.46 µg/mL, respectively. On the other hand, different results were obtained and Cotula cinerea essential oil was the most cytotoxic with the lowest IC50 value (72.72±2.18 µg/mL) against Vero cell line. In the same conditions, higher concentrations were needed in the case of Salvia verbenaca extracts. The results of this study showed thus that Cotula cinerea essential oil and hexane extract showed significant cytotoxic effects against RD and Vero cell lines, respectively, and could be considered as novel source of antitumor agents. This study is expected to be beneficial for clinical and traditional applications for Cotula cinerea as a remedy against cancer and opens new perspectives for further investigations on other types of cancer cell lines.http://dx.doi.org/10.1155/2018/7163961 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fatima-Ezzahrae Guaouguaou Mohamed Alien Ahl Bebaha Khalid Taghzouti Abdelhakim Bouyahya Youssef Bakri Nadia Dakka Nour Eddine Es-Safi |
spellingShingle |
Fatima-Ezzahrae Guaouguaou Mohamed Alien Ahl Bebaha Khalid Taghzouti Abdelhakim Bouyahya Youssef Bakri Nadia Dakka Nour Eddine Es-Safi Cytotoxicological Investigation of the Essential Oil and the Extracts of Cotula cinerea and Salvia verbenaca from Morocco BioMed Research International |
author_facet |
Fatima-Ezzahrae Guaouguaou Mohamed Alien Ahl Bebaha Khalid Taghzouti Abdelhakim Bouyahya Youssef Bakri Nadia Dakka Nour Eddine Es-Safi |
author_sort |
Fatima-Ezzahrae Guaouguaou |
title |
Cytotoxicological Investigation of the Essential Oil and the Extracts of Cotula cinerea and Salvia verbenaca from Morocco |
title_short |
Cytotoxicological Investigation of the Essential Oil and the Extracts of Cotula cinerea and Salvia verbenaca from Morocco |
title_full |
Cytotoxicological Investigation of the Essential Oil and the Extracts of Cotula cinerea and Salvia verbenaca from Morocco |
title_fullStr |
Cytotoxicological Investigation of the Essential Oil and the Extracts of Cotula cinerea and Salvia verbenaca from Morocco |
title_full_unstemmed |
Cytotoxicological Investigation of the Essential Oil and the Extracts of Cotula cinerea and Salvia verbenaca from Morocco |
title_sort |
cytotoxicological investigation of the essential oil and the extracts of cotula cinerea and salvia verbenaca from morocco |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2018-01-01 |
description |
The objective of this work was to investigate the cytotoxicological effect of the extracts (hexane, ethyl acetate, and n-butanol) of Cotula cinerea and Salvia verbenaca in addition to the essential oil of Cotula cinerea. These plants are widely used in the Moroccan traditional folk medicine. The cytotoxic effect was explored against two cancer cell lines, Vero and RD, using the colorimetric MTT assay. The obtained results showed that the cytotoxicity differed according to the used extract with an efficient effect of Cotula cinerea extracts compared to Salvia verbenaca. A potent cytotoxicity was thus observed for the Cotula cinerea hexane extract which inhibited the growth of RD cell line at the lowest IC50 value (57.21±3.43 µg/mL). This was followed by the ethyl acetate extract and the essential oil with moderate effects against RD cell line and showed IC50 values of 187.52±6.27 µg/mL and 173.05±4.46 µg/mL, respectively. On the other hand, different results were obtained and Cotula cinerea essential oil was the most cytotoxic with the lowest IC50 value (72.72±2.18 µg/mL) against Vero cell line. In the same conditions, higher concentrations were needed in the case of Salvia verbenaca extracts. The results of this study showed thus that Cotula cinerea essential oil and hexane extract showed significant cytotoxic effects against RD and Vero cell lines, respectively, and could be considered as novel source of antitumor agents. This study is expected to be beneficial for clinical and traditional applications for Cotula cinerea as a remedy against cancer and opens new perspectives for further investigations on other types of cancer cell lines. |
url |
http://dx.doi.org/10.1155/2018/7163961 |
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