Functionalized Thick Film Impedance Sensors for Use in In Vitro Cell Culture
Multi-electrode arrays find application in electrophysiological recordings. The quality of the captured signals depends on the interfacial contact between electrogenic cells and the electronic system. Therefore, it requires reliable low-impedance electrodes. Low-temperature cofired ceramic technolog...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2018-04-01
|
Series: | Biosensors |
Subjects: | |
Online Access: | http://www.mdpi.com/2079-6374/8/2/37 |
id |
doaj-227d1df29d6f4489b7362d59161e8db5 |
---|---|
record_format |
Article |
spelling |
doaj-227d1df29d6f4489b7362d59161e8db52020-11-25T02:25:38ZengMDPI AGBiosensors2079-63742018-04-01823710.3390/bios8020037bios8020037Functionalized Thick Film Impedance Sensors for Use in In Vitro Cell CultureHeike Bartsch0Martin Baca1Uta Fernekorn2Jens Müller3Andreas Schober4Hartmut Witte5Electronics Technology Group, Technische Universität Ilmenau, IMN MacroNano<sup>®</sup>, 98693 Ilmenau, GermanyNano-Biosystems Technology Group, Technische Universität Ilmenau, IMN MacroNano<sup>®</sup>, 98693 Ilmenau, GermanyNano-Biosystems Technology Group, Technische Universität Ilmenau, IMN MacroNano<sup>®</sup>, 98693 Ilmenau, GermanyElectronics Technology Group, Technische Universität Ilmenau, IMN MacroNano<sup>®</sup>, 98693 Ilmenau, GermanyNano-Biosystems Technology Group, Technische Universität Ilmenau, IMN MacroNano<sup>®</sup>, 98693 Ilmenau, GermanyBiomechatronics Group, Technische Universität Ilmenau, IMN MacroNano<sup>®</sup>, 98693 Ilmenau, GermanyMulti-electrode arrays find application in electrophysiological recordings. The quality of the captured signals depends on the interfacial contact between electrogenic cells and the electronic system. Therefore, it requires reliable low-impedance electrodes. Low-temperature cofired ceramic technology offers a suitable platform for rapid prototyping of biological reactors and can provide both stable fluid supply and integrated bio-hardware interfaces for recordings in electrogenic cell cultures. The 3D assembly of thick film gold electrodes in in vitro bio-reactors has been demonstrated for neuronal recordings. However, especially when dimensions become small, their performance varies strongly. This work investigates the influence of different coatings on thick film gold electrodes with regard to their influence on impedance behavior. PEDOT:PSS layer, titanium oxynitride and laminin coatings are deposited on LTCC gold electrodes using different 2D and 3D MEA chip designs. Their impedance characteristics are compared and discussed. Titanium oxynitride layers emerged as suitable functionalization. Small 86-µm-electrodes have a serial resistance Rs of 32 kOhm and serial capacitance Cs of 4.1 pF at 1 kHz. Thick film gold electrodes with such coatings are thus qualified for signal recording in 3-dimensional in vitro cell cultures.http://www.mdpi.com/2079-6374/8/2/37thick film sensorsimpedance measurementlow temperature co-fired ceramicsfunctionalizationelectrophysiological recordings |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Heike Bartsch Martin Baca Uta Fernekorn Jens Müller Andreas Schober Hartmut Witte |
spellingShingle |
Heike Bartsch Martin Baca Uta Fernekorn Jens Müller Andreas Schober Hartmut Witte Functionalized Thick Film Impedance Sensors for Use in In Vitro Cell Culture Biosensors thick film sensors impedance measurement low temperature co-fired ceramics functionalization electrophysiological recordings |
author_facet |
Heike Bartsch Martin Baca Uta Fernekorn Jens Müller Andreas Schober Hartmut Witte |
author_sort |
Heike Bartsch |
title |
Functionalized Thick Film Impedance Sensors for Use in In Vitro Cell Culture |
title_short |
Functionalized Thick Film Impedance Sensors for Use in In Vitro Cell Culture |
title_full |
Functionalized Thick Film Impedance Sensors for Use in In Vitro Cell Culture |
title_fullStr |
Functionalized Thick Film Impedance Sensors for Use in In Vitro Cell Culture |
title_full_unstemmed |
Functionalized Thick Film Impedance Sensors for Use in In Vitro Cell Culture |
title_sort |
functionalized thick film impedance sensors for use in in vitro cell culture |
publisher |
MDPI AG |
series |
Biosensors |
issn |
2079-6374 |
publishDate |
2018-04-01 |
description |
Multi-electrode arrays find application in electrophysiological recordings. The quality of the captured signals depends on the interfacial contact between electrogenic cells and the electronic system. Therefore, it requires reliable low-impedance electrodes. Low-temperature cofired ceramic technology offers a suitable platform for rapid prototyping of biological reactors and can provide both stable fluid supply and integrated bio-hardware interfaces for recordings in electrogenic cell cultures. The 3D assembly of thick film gold electrodes in in vitro bio-reactors has been demonstrated for neuronal recordings. However, especially when dimensions become small, their performance varies strongly. This work investigates the influence of different coatings on thick film gold electrodes with regard to their influence on impedance behavior. PEDOT:PSS layer, titanium oxynitride and laminin coatings are deposited on LTCC gold electrodes using different 2D and 3D MEA chip designs. Their impedance characteristics are compared and discussed. Titanium oxynitride layers emerged as suitable functionalization. Small 86-µm-electrodes have a serial resistance Rs of 32 kOhm and serial capacitance Cs of 4.1 pF at 1 kHz. Thick film gold electrodes with such coatings are thus qualified for signal recording in 3-dimensional in vitro cell cultures. |
topic |
thick film sensors impedance measurement low temperature co-fired ceramics functionalization electrophysiological recordings |
url |
http://www.mdpi.com/2079-6374/8/2/37 |
work_keys_str_mv |
AT heikebartsch functionalizedthickfilmimpedancesensorsforuseininvitrocellculture AT martinbaca functionalizedthickfilmimpedancesensorsforuseininvitrocellculture AT utafernekorn functionalizedthickfilmimpedancesensorsforuseininvitrocellculture AT jensmuller functionalizedthickfilmimpedancesensorsforuseininvitrocellculture AT andreasschober functionalizedthickfilmimpedancesensorsforuseininvitrocellculture AT hartmutwitte functionalizedthickfilmimpedancesensorsforuseininvitrocellculture |
_version_ |
1724850837269774336 |