CRISPR-induced exon skipping is dependent on premature termination codon mutations

• Main Authors: Tingting Sui, Yuning Song, Zhiquan Liu, Mao Chen, Jichao Deng, Yuanyuan Xu, Liangxue Lai, Zhanjun Li
• Format: Article
• Language: English
• Published: BMC 2018-10-01
• Series: Genome Biology
• Subjects: Exon skipping; PTC; CRISPR/Cas9; Rabbits
• Online Access: http://link.springer.com/article/10.1186/s13059-018-1532-z
• ISSN: 1474-760X

Abstract In previous studies, CRISPR/Cas9 was shown to induce unexpected exon skipping; however, the mechanism by which this phenomenon is triggered is controversial. By analyzing 22 gene-edited rabbit lines generated using CRISPR/Cas9, we provide evidence of exon skipping at high frequency in premature termination codon-mutated rabbits but not in the rabbits with a premature termination codon mutation in exon 1 rabbits with non-frameshift or missense mutations. Our results suggest that CRISPR-mediated exon skipping depends on premature termination codon mutation-induced nonsense-associated altered splicing.