Human Bocavirus Infection Markers in Peripheral Blood and Stool Samples of Children with Acute Gastroenteritis
Human bocaviruses (HBoVs) 1⁻4 belong to the Parvoviridae family, and they infect the respiratory or gastrointestinal tracts in children. We investigated the prevalence of HBoV1⁻4 DNAs in the blood and stool samples, and of HBoV1⁻4 IgG and IgM in the plasma samples, of c...
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doaj-2290e9732bf048388a154d94d1f470722020-11-25T02:09:17ZengMDPI AGViruses1999-49152018-11-01101163910.3390/v10110639v10110639Human Bocavirus Infection Markers in Peripheral Blood and Stool Samples of Children with Acute GastroenteritisZaiga Nora-Krukle0Anda Vilmane1Man Xu2Santa Rasa3Inga Ziemele4Elina Silina5Maria Söderlund-Venermo6Dace Gardovska7Modra Murovska8Institute of Microbiology and Virology, Rīga Stradiņš University, 5 Ratsupites St., LV-1067 Riga, LatviaInstitute of Microbiology and Virology, Rīga Stradiņš University, 5 Ratsupites St., LV-1067 Riga, LatviaDepartment of Virology, University of Helsinki, 3 (PL21), 00014 Helsinki, FinlandInstitute of Microbiology and Virology, Rīga Stradiņš University, 5 Ratsupites St., LV-1067 Riga, LatviaChildren’s Clinical University Hospital, 45 Vienibas Avenue, LV-1004 Riga, LatviaDepartment of Paediatrics, Rīga Stradiņš University, 16 Dzirciema St., LV-1067 Riga, LatviaDepartment of Virology, University of Helsinki, 3 (PL21), 00014 Helsinki, FinlandChildren’s Clinical University Hospital, 45 Vienibas Avenue, LV-1004 Riga, LatviaInstitute of Microbiology and Virology, Rīga Stradiņš University, 5 Ratsupites St., LV-1067 Riga, LatviaHuman bocaviruses (HBoVs) 1⁻4 belong to the Parvoviridae family, and they infect the respiratory or gastrointestinal tracts in children. We investigated the prevalence of HBoV1⁻4 DNAs in the blood and stool samples, and of HBoV1⁻4 IgG and IgM in the plasma samples, of children presenting with acute gastroenteritis (AGE). In addition, we identified HBoV co-infections with the five most frequent gastrointestinal pathogens. A total of 83 paired blood and stool samples were collected from children aged five years or less. Infection markers of HBoV1, 2, or 3 (viral DNA in blood and/or stool and/or antibodies) were detected in 61 out of 83 (73.5%) patients. HBoV1, 2, or 3 DNA as a monoinfection was revealed in 18.1%, 2.4%, and 1.2%, respectively, and 21.7% in total. In 56.1% of the HBoV DNA-positive patients, the presence in stool of another virus—most frequently norovirus or rotavirus—was observed. In conclusion, this study, for the first time, illustrates the prevalence and genetic diversity of HBoVs in Latvian children with gastroenteritis, and shows a widespread distribution of these viruses in the community. HBoV1 and 2 are commonly found as single infectious agents in children with AGE, suggesting that the viruses can be as pathogenic by themselves as other enteric agents are.https://www.mdpi.com/1999-4915/10/11/639human bocaviruschildrenacute gastroenteritis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zaiga Nora-Krukle Anda Vilmane Man Xu Santa Rasa Inga Ziemele Elina Silina Maria Söderlund-Venermo Dace Gardovska Modra Murovska |
spellingShingle |
Zaiga Nora-Krukle Anda Vilmane Man Xu Santa Rasa Inga Ziemele Elina Silina Maria Söderlund-Venermo Dace Gardovska Modra Murovska Human Bocavirus Infection Markers in Peripheral Blood and Stool Samples of Children with Acute Gastroenteritis Viruses human bocavirus children acute gastroenteritis |
author_facet |
Zaiga Nora-Krukle Anda Vilmane Man Xu Santa Rasa Inga Ziemele Elina Silina Maria Söderlund-Venermo Dace Gardovska Modra Murovska |
author_sort |
Zaiga Nora-Krukle |
title |
Human Bocavirus Infection Markers in Peripheral Blood and Stool Samples of Children with Acute Gastroenteritis |
title_short |
Human Bocavirus Infection Markers in Peripheral Blood and Stool Samples of Children with Acute Gastroenteritis |
title_full |
Human Bocavirus Infection Markers in Peripheral Blood and Stool Samples of Children with Acute Gastroenteritis |
title_fullStr |
Human Bocavirus Infection Markers in Peripheral Blood and Stool Samples of Children with Acute Gastroenteritis |
title_full_unstemmed |
Human Bocavirus Infection Markers in Peripheral Blood and Stool Samples of Children with Acute Gastroenteritis |
title_sort |
human bocavirus infection markers in peripheral blood and stool samples of children with acute gastroenteritis |
publisher |
MDPI AG |
series |
Viruses |
issn |
1999-4915 |
publishDate |
2018-11-01 |
description |
Human bocaviruses (HBoVs) 1⁻4 belong to the Parvoviridae family, and they infect the respiratory or gastrointestinal tracts in children. We investigated the prevalence of HBoV1⁻4 DNAs in the blood and stool samples, and of HBoV1⁻4 IgG and IgM in the plasma samples, of children presenting with acute gastroenteritis (AGE). In addition, we identified HBoV co-infections with the five most frequent gastrointestinal pathogens. A total of 83 paired blood and stool samples were collected from children aged five years or less. Infection markers of HBoV1, 2, or 3 (viral DNA in blood and/or stool and/or antibodies) were detected in 61 out of 83 (73.5%) patients. HBoV1, 2, or 3 DNA as a monoinfection was revealed in 18.1%, 2.4%, and 1.2%, respectively, and 21.7% in total. In 56.1% of the HBoV DNA-positive patients, the presence in stool of another virus—most frequently norovirus or rotavirus—was observed. In conclusion, this study, for the first time, illustrates the prevalence and genetic diversity of HBoVs in Latvian children with gastroenteritis, and shows a widespread distribution of these viruses in the community. HBoV1 and 2 are commonly found as single infectious agents in children with AGE, suggesting that the viruses can be as pathogenic by themselves as other enteric agents are. |
topic |
human bocavirus children acute gastroenteritis |
url |
https://www.mdpi.com/1999-4915/10/11/639 |
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