Epitope-loaded nanoemulsion delivery system with ability of extending antigen release elicits potent Th1 response for intranasal vaccine against Helicobacter pylori
Abstract Background Helicobacter pylori (H. pylori) infection remains a global public health issue, especially in Asia. Due to the emergence of antibiotic-resistant strains and the complexity of H. pylori infection, conventional vaccination is the best way to control the disease. Our previous study...
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doaj-22a881a8b10a455fb5acf5693b3ca0332020-11-25T01:23:32ZengBMCJournal of Nanobiotechnology1477-31552019-01-0117111510.1186/s12951-019-0441-yEpitope-loaded nanoemulsion delivery system with ability of extending antigen release elicits potent Th1 response for intranasal vaccine against Helicobacter pyloriYun Yang0Li Chen1Hong-wu Sun2Hong Guo3Zhen Song4Ying You5Liu-yang Yang6Ya-nan Tong7Ji-ning Gao8Hao Zeng9Wu-chen Yang10Quan-ming Zou11National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical UniversityNational Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical UniversityNational Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical UniversityDepartment of Gastroenterology, The Second Affiliated Hospital, Third Military Medical UniversityNational Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical UniversityNational Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical UniversityNational Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical UniversityNational Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical UniversityInstitute of Combined Injury of PLA, College of Military Preventive Medicine, Third Military Medical University of Chinese PLANational Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical UniversityNational Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical UniversityNational Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical UniversityAbstract Background Helicobacter pylori (H. pylori) infection remains a global public health issue, especially in Asia. Due to the emergence of antibiotic-resistant strains and the complexity of H. pylori infection, conventional vaccination is the best way to control the disease. Our previous study found that the N-acetyl-neuroaminyllactose-binding hemagglutinin protein (HpaA) is an effective protective antigen for vaccination against H. pylori infection, and intranasal immunization with the immunodominant HpaA epitope peptide (HpaA 154-171, P22, MEGVLIPAGFIKVTILEP) in conjunction with a CpG adjuvant decreased bacterial colonization in H. pylori-infected mice. However, to confer more robust and effective protection against H. pylori infection, an optimized delivery system is needed to enhance the P22-specific memory T cell response. Results In this study, an intranasal nanoemulsion (NE) delivery system offering high vaccine efficacy without obvious cytotoxicity was designed and produced. We found that this highly stable system significantly prolonged the nasal residence time and enhanced the cellular uptake of the epitope peptide, which powerfully boosted the specific Th1 responses of the NE-P22 vaccine, thus reducing bacterial colonization without CpG. Furthermore, the protection efficacy was further enhanced by combining the NE-P22 vaccine with CpG. Conclusion This epitope-loaded nanoemulsion delivery system was shown to extend antigen release and elicit potent Th1 response, it is an applicable delivery system for intranasal vaccine against H. pylori.http://link.springer.com/article/10.1186/s12951-019-0441-yNanoemulsionEpitope vaccineHelicobacter pyloriIntranasalHpaA |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yun Yang Li Chen Hong-wu Sun Hong Guo Zhen Song Ying You Liu-yang Yang Ya-nan Tong Ji-ning Gao Hao Zeng Wu-chen Yang Quan-ming Zou |
spellingShingle |
Yun Yang Li Chen Hong-wu Sun Hong Guo Zhen Song Ying You Liu-yang Yang Ya-nan Tong Ji-ning Gao Hao Zeng Wu-chen Yang Quan-ming Zou Epitope-loaded nanoemulsion delivery system with ability of extending antigen release elicits potent Th1 response for intranasal vaccine against Helicobacter pylori Journal of Nanobiotechnology Nanoemulsion Epitope vaccine Helicobacter pylori Intranasal HpaA |
author_facet |
Yun Yang Li Chen Hong-wu Sun Hong Guo Zhen Song Ying You Liu-yang Yang Ya-nan Tong Ji-ning Gao Hao Zeng Wu-chen Yang Quan-ming Zou |
author_sort |
Yun Yang |
title |
Epitope-loaded nanoemulsion delivery system with ability of extending antigen release elicits potent Th1 response for intranasal vaccine against Helicobacter pylori |
title_short |
Epitope-loaded nanoemulsion delivery system with ability of extending antigen release elicits potent Th1 response for intranasal vaccine against Helicobacter pylori |
title_full |
Epitope-loaded nanoemulsion delivery system with ability of extending antigen release elicits potent Th1 response for intranasal vaccine against Helicobacter pylori |
title_fullStr |
Epitope-loaded nanoemulsion delivery system with ability of extending antigen release elicits potent Th1 response for intranasal vaccine against Helicobacter pylori |
title_full_unstemmed |
Epitope-loaded nanoemulsion delivery system with ability of extending antigen release elicits potent Th1 response for intranasal vaccine against Helicobacter pylori |
title_sort |
epitope-loaded nanoemulsion delivery system with ability of extending antigen release elicits potent th1 response for intranasal vaccine against helicobacter pylori |
publisher |
BMC |
series |
Journal of Nanobiotechnology |
issn |
1477-3155 |
publishDate |
2019-01-01 |
description |
Abstract Background Helicobacter pylori (H. pylori) infection remains a global public health issue, especially in Asia. Due to the emergence of antibiotic-resistant strains and the complexity of H. pylori infection, conventional vaccination is the best way to control the disease. Our previous study found that the N-acetyl-neuroaminyllactose-binding hemagglutinin protein (HpaA) is an effective protective antigen for vaccination against H. pylori infection, and intranasal immunization with the immunodominant HpaA epitope peptide (HpaA 154-171, P22, MEGVLIPAGFIKVTILEP) in conjunction with a CpG adjuvant decreased bacterial colonization in H. pylori-infected mice. However, to confer more robust and effective protection against H. pylori infection, an optimized delivery system is needed to enhance the P22-specific memory T cell response. Results In this study, an intranasal nanoemulsion (NE) delivery system offering high vaccine efficacy without obvious cytotoxicity was designed and produced. We found that this highly stable system significantly prolonged the nasal residence time and enhanced the cellular uptake of the epitope peptide, which powerfully boosted the specific Th1 responses of the NE-P22 vaccine, thus reducing bacterial colonization without CpG. Furthermore, the protection efficacy was further enhanced by combining the NE-P22 vaccine with CpG. Conclusion This epitope-loaded nanoemulsion delivery system was shown to extend antigen release and elicit potent Th1 response, it is an applicable delivery system for intranasal vaccine against H. pylori. |
topic |
Nanoemulsion Epitope vaccine Helicobacter pylori Intranasal HpaA |
url |
http://link.springer.com/article/10.1186/s12951-019-0441-y |
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