Microarray-Based Comparisons of Ion Channel Expression Patterns: Human Keratinocytes to Reprogrammed hiPSCs to Differentiated Neuronal and Cardiac Progeny
Ion channels are involved in a large variety of cellular processes including stem cell differentiation. Numerous families of ion channels are present in the organism which can be distinguished by means of, for example, ion selectivity, gating mechanism, composition, or cell biological function. To c...
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Online Access: | http://dx.doi.org/10.1155/2013/784629 |
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doaj-22af6c40ef2e4745b80ca22015e2510b2020-11-24T22:01:44ZengHindawi LimitedStem Cells International1687-966X1687-96782013-01-01201310.1155/2013/784629784629Microarray-Based Comparisons of Ion Channel Expression Patterns: Human Keratinocytes to Reprogrammed hiPSCs to Differentiated Neuronal and Cardiac ProgenyLeonhard Linta0Marianne Stockmann1Qiong Lin2André Lechel3Christian Proepper4Tobias M. Boeckers5Alexander Kleger6Stefan Liebau7Institute for Anatomy Cell Biology, Ulm University, Albert-Einstein Allee 11, 89081 Ulm, GermanyInstitute for Anatomy Cell Biology, Ulm University, Albert-Einstein Allee 11, 89081 Ulm, GermanyInstitute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen, Pauwelstrasse 30, 52074 Aachen, GermanyDepartment of Internal Medicine I, Ulm University, Albert-Einstein Allee 11, 89081 Ulm, GermanyInstitute for Anatomy Cell Biology, Ulm University, Albert-Einstein Allee 11, 89081 Ulm, GermanyInstitute for Anatomy Cell Biology, Ulm University, Albert-Einstein Allee 11, 89081 Ulm, GermanyDepartment of Internal Medicine I, Ulm University, Albert-Einstein Allee 11, 89081 Ulm, GermanyInstitute for Anatomy Cell Biology, Ulm University, Albert-Einstein Allee 11, 89081 Ulm, GermanyIon channels are involved in a large variety of cellular processes including stem cell differentiation. Numerous families of ion channels are present in the organism which can be distinguished by means of, for example, ion selectivity, gating mechanism, composition, or cell biological function. To characterize the distinct expression of this group of ion channels we have compared the mRNA expression levels of ion channel genes between human keratinocyte-derived induced pluripotent stem cells (hiPSCs) and their somatic cell source, keratinocytes from plucked human hair. This comparison revealed that 26% of the analyzed probes showed an upregulation of ion channels in hiPSCs while just 6% were downregulated. Additionally, iPSCs express a much higher number of ion channels compared to keratinocytes. Further, to narrow down specificity of ion channel expression in iPS cells we compared their expression patterns with differentiated progeny, namely, neurons and cardiomyocytes derived from iPS cells. To conclude, hiPSCs exhibit a very considerable and diverse ion channel expression pattern. Their detailed analysis could give an insight into their contribution to many cellular processes and even disease mechanisms.http://dx.doi.org/10.1155/2013/784629 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Leonhard Linta Marianne Stockmann Qiong Lin André Lechel Christian Proepper Tobias M. Boeckers Alexander Kleger Stefan Liebau |
spellingShingle |
Leonhard Linta Marianne Stockmann Qiong Lin André Lechel Christian Proepper Tobias M. Boeckers Alexander Kleger Stefan Liebau Microarray-Based Comparisons of Ion Channel Expression Patterns: Human Keratinocytes to Reprogrammed hiPSCs to Differentiated Neuronal and Cardiac Progeny Stem Cells International |
author_facet |
Leonhard Linta Marianne Stockmann Qiong Lin André Lechel Christian Proepper Tobias M. Boeckers Alexander Kleger Stefan Liebau |
author_sort |
Leonhard Linta |
title |
Microarray-Based Comparisons of Ion Channel Expression Patterns: Human Keratinocytes to Reprogrammed hiPSCs to Differentiated Neuronal and Cardiac Progeny |
title_short |
Microarray-Based Comparisons of Ion Channel Expression Patterns: Human Keratinocytes to Reprogrammed hiPSCs to Differentiated Neuronal and Cardiac Progeny |
title_full |
Microarray-Based Comparisons of Ion Channel Expression Patterns: Human Keratinocytes to Reprogrammed hiPSCs to Differentiated Neuronal and Cardiac Progeny |
title_fullStr |
Microarray-Based Comparisons of Ion Channel Expression Patterns: Human Keratinocytes to Reprogrammed hiPSCs to Differentiated Neuronal and Cardiac Progeny |
title_full_unstemmed |
Microarray-Based Comparisons of Ion Channel Expression Patterns: Human Keratinocytes to Reprogrammed hiPSCs to Differentiated Neuronal and Cardiac Progeny |
title_sort |
microarray-based comparisons of ion channel expression patterns: human keratinocytes to reprogrammed hipscs to differentiated neuronal and cardiac progeny |
publisher |
Hindawi Limited |
series |
Stem Cells International |
issn |
1687-966X 1687-9678 |
publishDate |
2013-01-01 |
description |
Ion channels are involved in a large variety of cellular processes including stem cell differentiation. Numerous families of ion channels are present in the organism which can be distinguished by means of, for example, ion selectivity, gating mechanism, composition, or cell biological function. To characterize the distinct expression of this group of ion channels we have compared the mRNA expression levels of ion channel genes between human keratinocyte-derived induced pluripotent stem cells (hiPSCs) and their somatic cell source, keratinocytes from plucked human hair. This comparison revealed that 26% of the analyzed probes showed an upregulation of ion channels in hiPSCs while just 6% were downregulated. Additionally, iPSCs express a much higher number of ion channels compared to keratinocytes. Further, to narrow down specificity of ion channel expression in iPS cells we compared their expression patterns with differentiated progeny, namely, neurons and cardiomyocytes derived from iPS cells. To conclude, hiPSCs exhibit a very considerable and diverse ion channel expression pattern. Their detailed analysis could give an insight into their contribution to many cellular processes and even disease mechanisms. |
url |
http://dx.doi.org/10.1155/2013/784629 |
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