Microfluidic-Based Mechanical Phenotyping of Androgen-Sensitive and Non-sensitive Prostate Cancer Cells Lines

Cell mechanical properties have been identified to characterize cells pathologic states. Here, we report our work on high-throughput mechanical phenotyping of androgen-sensitive and non-sensitive human prostate cancer cell lines based on a morphological rheological microfluidic method. The theory fo...

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Main Authors:	Na Liu, Panpan Du, Xiaoxiao Xiao, Yuanyuan Liu, Yan Peng, Chen Yang, Tao Yue
Format:	Article
Language:	English
Published:	MDPI AG 2019-09-01
Series:	Micromachines
Subjects:	cellular mechanics cell deformability single-cell analysis high-throughput microfluidic morphological rheology
Online Access:	https://www.mdpi.com/2072-666X/10/9/602

id	doaj-22ba0b13e71642f4b8f32e440db74742
record_format	Article
spelling	doaj-22ba0b13e71642f4b8f32e440db747422020-11-25T02:03:26ZengMDPI AGMicromachines2072-666X2019-09-0110960210.3390/mi10090602mi10090602Microfluidic-Based Mechanical Phenotyping of Androgen-Sensitive and Non-sensitive Prostate Cancer Cells LinesNa Liu0Panpan Du1Xiaoxiao Xiao2Yuanyuan Liu3Yan Peng4Chen Yang5Tao Yue6School of Mechatronics Engineering and Automation, Shanghai University, Shanghai 200444, ChinaSchool of Mechatronics Engineering and Automation, Shanghai University, Shanghai 200444, ChinaSchool of Mechatronics Engineering and Automation, Shanghai University, Shanghai 200444, ChinaSchool of Mechatronics Engineering and Automation, Shanghai University, Shanghai 200444, ChinaSchool of Mechatronics Engineering and Automation, Shanghai University, Shanghai 200444, ChinaFudan Institute of Urology, Fudan University, Shanghai 200433, ChinaSchool of Mechatronics Engineering and Automation, Shanghai University, Shanghai 200444, ChinaCell mechanical properties have been identified to characterize cells pathologic states. Here, we report our work on high-throughput mechanical phenotyping of androgen-sensitive and non-sensitive human prostate cancer cell lines based on a morphological rheological microfluidic method. The theory for extracting cells’ elastic modulus from their deformation and area, and the used experimental parameters were analyzed. The mechanical properties of three types of prostate cancer cells lines with different sensitivity to androgen including LNCaP, DU145, and PC3 were quantified. The result shows that LNCaP cell was the softest, DU145 was the second softest, and PC3 was the stiffest. Furthermore, atomic force microscopy (AFM) was used to verify the effectiveness of this high-throughput morphological rheological method.https://www.mdpi.com/2072-666X/10/9/602cellular mechanicscell deformabilitysingle-cell analysishigh-throughputmicrofluidicmorphological rheology
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Na Liu Panpan Du Xiaoxiao Xiao Yuanyuan Liu Yan Peng Chen Yang Tao Yue
spellingShingle	Na Liu Panpan Du Xiaoxiao Xiao Yuanyuan Liu Yan Peng Chen Yang Tao Yue Microfluidic-Based Mechanical Phenotyping of Androgen-Sensitive and Non-sensitive Prostate Cancer Cells Lines Micromachines cellular mechanics cell deformability single-cell analysis high-throughput microfluidic morphological rheology
author_facet	Na Liu Panpan Du Xiaoxiao Xiao Yuanyuan Liu Yan Peng Chen Yang Tao Yue
author_sort	Na Liu
title	Microfluidic-Based Mechanical Phenotyping of Androgen-Sensitive and Non-sensitive Prostate Cancer Cells Lines
title_short	Microfluidic-Based Mechanical Phenotyping of Androgen-Sensitive and Non-sensitive Prostate Cancer Cells Lines
title_full	Microfluidic-Based Mechanical Phenotyping of Androgen-Sensitive and Non-sensitive Prostate Cancer Cells Lines
title_fullStr	Microfluidic-Based Mechanical Phenotyping of Androgen-Sensitive and Non-sensitive Prostate Cancer Cells Lines
title_full_unstemmed	Microfluidic-Based Mechanical Phenotyping of Androgen-Sensitive and Non-sensitive Prostate Cancer Cells Lines
title_sort	microfluidic-based mechanical phenotyping of androgen-sensitive and non-sensitive prostate cancer cells lines
publisher	MDPI AG
series	Micromachines
issn	2072-666X
publishDate	2019-09-01
description	Cell mechanical properties have been identified to characterize cells pathologic states. Here, we report our work on high-throughput mechanical phenotyping of androgen-sensitive and non-sensitive human prostate cancer cell lines based on a morphological rheological microfluidic method. The theory for extracting cells’ elastic modulus from their deformation and area, and the used experimental parameters were analyzed. The mechanical properties of three types of prostate cancer cells lines with different sensitivity to androgen including LNCaP, DU145, and PC3 were quantified. The result shows that LNCaP cell was the softest, DU145 was the second softest, and PC3 was the stiffest. Furthermore, atomic force microscopy (AFM) was used to verify the effectiveness of this high-throughput morphological rheological method.
topic	cellular mechanics cell deformability single-cell analysis high-throughput microfluidic morphological rheology
url	https://www.mdpi.com/2072-666X/10/9/602
work_keys_str_mv	AT naliu microfluidicbasedmechanicalphenotypingofandrogensensitiveandnonsensitiveprostatecancercellslines AT panpandu microfluidicbasedmechanicalphenotypingofandrogensensitiveandnonsensitiveprostatecancercellslines AT xiaoxiaoxiao microfluidicbasedmechanicalphenotypingofandrogensensitiveandnonsensitiveprostatecancercellslines AT yuanyuanliu microfluidicbasedmechanicalphenotypingofandrogensensitiveandnonsensitiveprostatecancercellslines AT yanpeng microfluidicbasedmechanicalphenotypingofandrogensensitiveandnonsensitiveprostatecancercellslines AT chenyang microfluidicbasedmechanicalphenotypingofandrogensensitiveandnonsensitiveprostatecancercellslines AT taoyue microfluidicbasedmechanicalphenotypingofandrogensensitiveandnonsensitiveprostatecancercellslines
_version_	1724948214629531648

Microfluidic-Based Mechanical Phenotyping of Androgen-Sensitive and Non-sensitive Prostate Cancer Cells Lines

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