Induction of Synovitis Using Interleukin-1 Beta: Are There Differences in the Response of Middle Carpal Joint Compared to the Tibiotarsal Joint?
Background: The effects of recombinant interleukin-1β (rIL-1β) have been described for the middle carpal joint (MCJ). However, we are unaware of any studies that have described the cytological response of the tibiotarsal joint (TTJ) to rIL-1β or compared the clinical and cytological responses of the...
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doaj-22ce5e236da74fbbac961d595484d4112020-11-24T21:28:28ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692018-08-01510.3389/fvets.2018.00208395409Induction of Synovitis Using Interleukin-1 Beta: Are There Differences in the Response of Middle Carpal Joint Compared to the Tibiotarsal Joint?Aimee C. Colbath0Aimee C. Colbath1Steven W. Dow2Leone S. Hopkins3Jennifer N. Phillips4Jennifer N. Phillips5C. Wayne McIlwraith6C. Wayne McIlwraith7Laurie R. Goodrich8Laurie R. Goodrich9Department of Clinical Sciences, Orthopaedic Research Center, Colorado State University, Fort Collins, CO, United StatesClinical Sciences, Colorado State University, Fort Collins, CO, United StatesClinical Sciences, Colorado State University, Fort Collins, CO, United StatesClinical Sciences, Colorado State University, Fort Collins, CO, United StatesDepartment of Clinical Sciences, Orthopaedic Research Center, Colorado State University, Fort Collins, CO, United StatesClinical Sciences, Colorado State University, Fort Collins, CO, United StatesDepartment of Clinical Sciences, Orthopaedic Research Center, Colorado State University, Fort Collins, CO, United StatesClinical Sciences, Colorado State University, Fort Collins, CO, United StatesDepartment of Clinical Sciences, Orthopaedic Research Center, Colorado State University, Fort Collins, CO, United StatesClinical Sciences, Colorado State University, Fort Collins, CO, United StatesBackground: The effects of recombinant interleukin-1β (rIL-1β) have been described for the middle carpal joint (MCJ). However, we are unaware of any studies that have described the cytological response of the tibiotarsal joint (TTJ) to rIL-1β or compared the clinical and cytological responses of the MCJ to the TTJ following the administration of intra-articular rIL-1β. Such information is critical for researchers planning to use rIL-1β to create acute synovitis models in horses.Objectives: To compare the clinical and cytological responses of the MCJ to the TTJ following administration of intra-articular rIL-1β.Methods: Twelve horses were used for the study. Eight horses received 75 ng of rIL-1β into the MCJ and four horses received 75 ng of rIL-1β into the TTJ. Clinical and cytological outcome parameters including lameness, joint circumference, joint effusion score, total nucleated cell count, cellular differentials, C-reactive protein, and prostaglandin-E2 concentrations were determined at baseline and multiple post-treatment time points over a 336 h period (2 weeks).Results: Recombinant IL-1β administered into the TTJ resulted in a significantly greater respiratory rate at 24 h and heart rate at 12 h when compared to rIL-1β administered into the MCJ. In addition, the TTJ had a significantly greater increase in joint circumference at 24 post-injection hour (PIH) and subjective effusion grade at 24 PIH and 336 PIH. The MCJ had significantly higher total protein concentration at 6 PIH, and a significantly higher NCC at 24 and 72 PIH when compared to the TTJ. Conversely, the TTJ had significantly higher neutrophilic infiltration than the MCJ at 6 PIH and 168 PIH.Conclusions: This study establishes that the same intra-articular dose of rIL-1 β elicits significantly different clinical and cytological responses in the MCJ compared to the TTJ in the equine model of intra-articular synovitis. In addition, clinical and cytological evidence of synovitis may persist up to or >1 week following intra-articular administration of rIL-1 β.https://www.frontiersin.org/article/10.3389/fvets.2018.00208/fullinterleukin-1 betacarpaltibiotarsalhorsessynovitis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Aimee C. Colbath Aimee C. Colbath Steven W. Dow Leone S. Hopkins Jennifer N. Phillips Jennifer N. Phillips C. Wayne McIlwraith C. Wayne McIlwraith Laurie R. Goodrich Laurie R. Goodrich |
spellingShingle |
Aimee C. Colbath Aimee C. Colbath Steven W. Dow Leone S. Hopkins Jennifer N. Phillips Jennifer N. Phillips C. Wayne McIlwraith C. Wayne McIlwraith Laurie R. Goodrich Laurie R. Goodrich Induction of Synovitis Using Interleukin-1 Beta: Are There Differences in the Response of Middle Carpal Joint Compared to the Tibiotarsal Joint? Frontiers in Veterinary Science interleukin-1 beta carpal tibiotarsal horses synovitis |
author_facet |
Aimee C. Colbath Aimee C. Colbath Steven W. Dow Leone S. Hopkins Jennifer N. Phillips Jennifer N. Phillips C. Wayne McIlwraith C. Wayne McIlwraith Laurie R. Goodrich Laurie R. Goodrich |
author_sort |
Aimee C. Colbath |
title |
Induction of Synovitis Using Interleukin-1 Beta: Are There Differences in the Response of Middle Carpal Joint Compared to the Tibiotarsal Joint? |
title_short |
Induction of Synovitis Using Interleukin-1 Beta: Are There Differences in the Response of Middle Carpal Joint Compared to the Tibiotarsal Joint? |
title_full |
Induction of Synovitis Using Interleukin-1 Beta: Are There Differences in the Response of Middle Carpal Joint Compared to the Tibiotarsal Joint? |
title_fullStr |
Induction of Synovitis Using Interleukin-1 Beta: Are There Differences in the Response of Middle Carpal Joint Compared to the Tibiotarsal Joint? |
title_full_unstemmed |
Induction of Synovitis Using Interleukin-1 Beta: Are There Differences in the Response of Middle Carpal Joint Compared to the Tibiotarsal Joint? |
title_sort |
induction of synovitis using interleukin-1 beta: are there differences in the response of middle carpal joint compared to the tibiotarsal joint? |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Veterinary Science |
issn |
2297-1769 |
publishDate |
2018-08-01 |
description |
Background: The effects of recombinant interleukin-1β (rIL-1β) have been described for the middle carpal joint (MCJ). However, we are unaware of any studies that have described the cytological response of the tibiotarsal joint (TTJ) to rIL-1β or compared the clinical and cytological responses of the MCJ to the TTJ following the administration of intra-articular rIL-1β. Such information is critical for researchers planning to use rIL-1β to create acute synovitis models in horses.Objectives: To compare the clinical and cytological responses of the MCJ to the TTJ following administration of intra-articular rIL-1β.Methods: Twelve horses were used for the study. Eight horses received 75 ng of rIL-1β into the MCJ and four horses received 75 ng of rIL-1β into the TTJ. Clinical and cytological outcome parameters including lameness, joint circumference, joint effusion score, total nucleated cell count, cellular differentials, C-reactive protein, and prostaglandin-E2 concentrations were determined at baseline and multiple post-treatment time points over a 336 h period (2 weeks).Results: Recombinant IL-1β administered into the TTJ resulted in a significantly greater respiratory rate at 24 h and heart rate at 12 h when compared to rIL-1β administered into the MCJ. In addition, the TTJ had a significantly greater increase in joint circumference at 24 post-injection hour (PIH) and subjective effusion grade at 24 PIH and 336 PIH. The MCJ had significantly higher total protein concentration at 6 PIH, and a significantly higher NCC at 24 and 72 PIH when compared to the TTJ. Conversely, the TTJ had significantly higher neutrophilic infiltration than the MCJ at 6 PIH and 168 PIH.Conclusions: This study establishes that the same intra-articular dose of rIL-1 β elicits significantly different clinical and cytological responses in the MCJ compared to the TTJ in the equine model of intra-articular synovitis. In addition, clinical and cytological evidence of synovitis may persist up to or >1 week following intra-articular administration of rIL-1 β. |
topic |
interleukin-1 beta carpal tibiotarsal horses synovitis |
url |
https://www.frontiersin.org/article/10.3389/fvets.2018.00208/full |
work_keys_str_mv |
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