Data on protein changes of chick vitreous during normal eye growth using data-independent acquisition (SWATH-MS)
Myopia is the most common refractive error which is estimated to affect half the population of the world by 2050. It has been suggested that it could be determined by multiple factors such as environmental and genetic, but the mechanism behind the cause of myopia is still yet to be identified. Vitre...
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doaj-22e5984b74ca4117b1c2b1d78fcd88a82020-11-25T03:11:29ZengElsevierData in Brief2352-34092020-06-0130105576Data on protein changes of chick vitreous during normal eye growth using data-independent acquisition (SWATH-MS)Jimmy Ka-Wai Cheung0King-Kit Li1Lei Zhou2Chi-Ho To3Thomas Chuen Lam4Laboratory of Experimental Optometry, Centre for Myopia Research, School of Optometry, The Hong Kong Polytechnic University, Hong Kong Special Administractive Region of ChinaLaboratory of Experimental Optometry, Centre for Myopia Research, School of Optometry, The Hong Kong Polytechnic University, Hong Kong Special Administractive Region of ChinaOcular Proteomics Laboratory, Singapore Eye Research Institute, Singapore; Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore; Ophthalmology and Visual Sciences Academic Clinical Research Program, Duke-NUS Medical School, National University of Singapore, SingaporeLaboratory of Experimental Optometry, Centre for Myopia Research, School of Optometry, The Hong Kong Polytechnic University, Hong Kong Special Administractive Region of ChinaLaboratory of Experimental Optometry, Centre for Myopia Research, School of Optometry, The Hong Kong Polytechnic University, Hong Kong Special Administractive Region of China; Corresponding author.Myopia is the most common refractive error which is estimated to affect half the population of the world by 2050. It has been suggested that it could be determined by multiple factors such as environmental and genetic, but the mechanism behind the cause of myopia is still yet to be identified. Vitreous humor (VH) is a transparent gelatin-like substance that takes up to 80% of the volume of the eye, making it the largest component of the eye. Although VH is the main contributor to axial elongation of the eye including normal eye growth (emmetropization) and myopia, the diluted nature of VH (made up of 99% of water) made it difficult for less abundant molecules to be identified and therefore often overlooked. Using the more sensitive label-free mass spectrometry approach with data-independent acquisition (SWATH-MS), we established a comprehensive VH proteome library in chick animal model and quantified possible protein biomarkers that are responsible for the axial elongation during emmetropization (7, 14, 21, 28 days after hatching, n = 48 eyes). Raw data files for both information-dependent acquisition (IDA) and data-independent acquisition (SWATH-MS) were uploaded on PeptideAtlas for public access (http://www.peptideatlas.org/PASS/PASS01258).http://www.sciencedirect.com/science/article/pii/S2352340920304704VitreousSWATHEmmetropizationChick |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jimmy Ka-Wai Cheung King-Kit Li Lei Zhou Chi-Ho To Thomas Chuen Lam |
spellingShingle |
Jimmy Ka-Wai Cheung King-Kit Li Lei Zhou Chi-Ho To Thomas Chuen Lam Data on protein changes of chick vitreous during normal eye growth using data-independent acquisition (SWATH-MS) Data in Brief Vitreous SWATH Emmetropization Chick |
author_facet |
Jimmy Ka-Wai Cheung King-Kit Li Lei Zhou Chi-Ho To Thomas Chuen Lam |
author_sort |
Jimmy Ka-Wai Cheung |
title |
Data on protein changes of chick vitreous during normal eye growth using data-independent acquisition (SWATH-MS) |
title_short |
Data on protein changes of chick vitreous during normal eye growth using data-independent acquisition (SWATH-MS) |
title_full |
Data on protein changes of chick vitreous during normal eye growth using data-independent acquisition (SWATH-MS) |
title_fullStr |
Data on protein changes of chick vitreous during normal eye growth using data-independent acquisition (SWATH-MS) |
title_full_unstemmed |
Data on protein changes of chick vitreous during normal eye growth using data-independent acquisition (SWATH-MS) |
title_sort |
data on protein changes of chick vitreous during normal eye growth using data-independent acquisition (swath-ms) |
publisher |
Elsevier |
series |
Data in Brief |
issn |
2352-3409 |
publishDate |
2020-06-01 |
description |
Myopia is the most common refractive error which is estimated to affect half the population of the world by 2050. It has been suggested that it could be determined by multiple factors such as environmental and genetic, but the mechanism behind the cause of myopia is still yet to be identified. Vitreous humor (VH) is a transparent gelatin-like substance that takes up to 80% of the volume of the eye, making it the largest component of the eye. Although VH is the main contributor to axial elongation of the eye including normal eye growth (emmetropization) and myopia, the diluted nature of VH (made up of 99% of water) made it difficult for less abundant molecules to be identified and therefore often overlooked. Using the more sensitive label-free mass spectrometry approach with data-independent acquisition (SWATH-MS), we established a comprehensive VH proteome library in chick animal model and quantified possible protein biomarkers that are responsible for the axial elongation during emmetropization (7, 14, 21, 28 days after hatching, n = 48 eyes). Raw data files for both information-dependent acquisition (IDA) and data-independent acquisition (SWATH-MS) were uploaded on PeptideAtlas for public access (http://www.peptideatlas.org/PASS/PASS01258). |
topic |
Vitreous SWATH Emmetropization Chick |
url |
http://www.sciencedirect.com/science/article/pii/S2352340920304704 |
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