Consistency and Variability of DNA Methylation in Women During Puberty, Young Adulthood, and Pregnancy
Prior DNA methylation (DNA-m) analyses have identified cytosine-phosphate-guanine (CpG) sites, which show either a significant change or consistency during lifetime. However, the proportion of CpGs that are neither significantly different nor consistent over time (indifferent CpGs) is unknown. We in...
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doaj-22f9db3ab62f439eafc2e52d84883f9b2020-11-24T21:04:32ZengSAGE PublishingGenetics and Epigenetics1179-237X2017-07-01910.1177/1179237X17721540Consistency and Variability of DNA Methylation in Women During Puberty, Young Adulthood, and PregnancySu Chen0Nandini Mukherjee1Vimala Devi Janjanam2S Hasan Arshad3Ramesh J Kurukulaaratchy4John W Holloway5Hongmei Zhang6Wilfried Karmaus7Department of Mathematical Sciences, The University of Memphis, Memphis, TN, USADivision of Epidemiology, Biostatistics, and Environmental Health, School of Public Health, The University of Memphis, Memphis, TN, USADivision of Epidemiology, Biostatistics, and Environmental Health, School of Public Health, The University of Memphis, Memphis, TN, USAThe David Hide Asthma and Allergy Research Centre, Newport, UKThe David Hide Asthma and Allergy Research Centre, Newport, UKAcademic Unit of Human Development and Health, Faculty of Medicine, University of Southampton, Southampton, UKDivision of Epidemiology, Biostatistics, and Environmental Health, School of Public Health, The University of Memphis, Memphis, TN, USADivision of Epidemiology, Biostatistics, and Environmental Health, School of Public Health, The University of Memphis, Memphis, TN, USAPrior DNA methylation (DNA-m) analyses have identified cytosine-phosphate-guanine (CpG) sites, which show either a significant change or consistency during lifetime. However, the proportion of CpGs that are neither significantly different nor consistent over time (indifferent CpGs) is unknown. We investigated the methylation dynamics, both longitudinal changes and consistency, in women from preadolescence to late pregnancy using DNA-m of peripheral blood cells. Consistency of cell type–adjusted DNA-m between paired individuals was assessed by regressing CpGs of subsequent age on the prior, stability by intraclass correlation coefficients (>0.5), and changes by linear mixed models. In the first 2 transitions (10-18 years and 18 years to early pregnancy), 19.5% and 20.9% CpGs were consistent, but only 0.35% in the third transition (from early to late pregnancy). Significant changes in methylation were found in 0.7%, 5.6%, and 0% CpGs, respectively. Functional enrichment analyses of genes with significant changes in DNA-m in early pregnancy (5.6%) showed that the maternal DNA-m seems to reflect signaling pathways between the uterus and the trophoblast. The transition from early to late pregnancy showed low consistency/stability and no changes, suggesting the presence of a large proportion of indifferent CpGs in late pregnancy.https://dx.doi.org/10.1177/1179237X17721540 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Su Chen Nandini Mukherjee Vimala Devi Janjanam S Hasan Arshad Ramesh J Kurukulaaratchy John W Holloway Hongmei Zhang Wilfried Karmaus |
spellingShingle |
Su Chen Nandini Mukherjee Vimala Devi Janjanam S Hasan Arshad Ramesh J Kurukulaaratchy John W Holloway Hongmei Zhang Wilfried Karmaus Consistency and Variability of DNA Methylation in Women During Puberty, Young Adulthood, and Pregnancy Genetics and Epigenetics |
author_facet |
Su Chen Nandini Mukherjee Vimala Devi Janjanam S Hasan Arshad Ramesh J Kurukulaaratchy John W Holloway Hongmei Zhang Wilfried Karmaus |
author_sort |
Su Chen |
title |
Consistency and Variability of DNA Methylation in Women During Puberty, Young Adulthood, and Pregnancy |
title_short |
Consistency and Variability of DNA Methylation in Women During Puberty, Young Adulthood, and Pregnancy |
title_full |
Consistency and Variability of DNA Methylation in Women During Puberty, Young Adulthood, and Pregnancy |
title_fullStr |
Consistency and Variability of DNA Methylation in Women During Puberty, Young Adulthood, and Pregnancy |
title_full_unstemmed |
Consistency and Variability of DNA Methylation in Women During Puberty, Young Adulthood, and Pregnancy |
title_sort |
consistency and variability of dna methylation in women during puberty, young adulthood, and pregnancy |
publisher |
SAGE Publishing |
series |
Genetics and Epigenetics |
issn |
1179-237X |
publishDate |
2017-07-01 |
description |
Prior DNA methylation (DNA-m) analyses have identified cytosine-phosphate-guanine (CpG) sites, which show either a significant change or consistency during lifetime. However, the proportion of CpGs that are neither significantly different nor consistent over time (indifferent CpGs) is unknown. We investigated the methylation dynamics, both longitudinal changes and consistency, in women from preadolescence to late pregnancy using DNA-m of peripheral blood cells. Consistency of cell type–adjusted DNA-m between paired individuals was assessed by regressing CpGs of subsequent age on the prior, stability by intraclass correlation coefficients (>0.5), and changes by linear mixed models. In the first 2 transitions (10-18 years and 18 years to early pregnancy), 19.5% and 20.9% CpGs were consistent, but only 0.35% in the third transition (from early to late pregnancy). Significant changes in methylation were found in 0.7%, 5.6%, and 0% CpGs, respectively. Functional enrichment analyses of genes with significant changes in DNA-m in early pregnancy (5.6%) showed that the maternal DNA-m seems to reflect signaling pathways between the uterus and the trophoblast. The transition from early to late pregnancy showed low consistency/stability and no changes, suggesting the presence of a large proportion of indifferent CpGs in late pregnancy. |
url |
https://dx.doi.org/10.1177/1179237X17721540 |
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