5-Methylcytosine and 5-hydroxymethylcytosine spatiotemporal profiles in the mouse zygote.
BACKGROUND: In the mouse zygote, DNA methylation patterns are heavily modified, and differ between the maternal and paternal pronucleus. Demethylation of the paternal genome has been described as an active and replication-independent process, although the mechanisms responsible for it remain elusive...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2012-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3364968?pdf=render |
id |
doaj-2300a2419ebe41f6b2991066b71d18ab |
---|---|
record_format |
Article |
spelling |
doaj-2300a2419ebe41f6b2991066b71d18ab2020-11-25T01:53:28ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0175e3815610.1371/journal.pone.00381565-Methylcytosine and 5-hydroxymethylcytosine spatiotemporal profiles in the mouse zygote.Juliette SalvaingTiphaine Aguirre-LavinClaire BoulesteixGaëtan LehmannPascale DebeyNathalie BeaujeanBACKGROUND: In the mouse zygote, DNA methylation patterns are heavily modified, and differ between the maternal and paternal pronucleus. Demethylation of the paternal genome has been described as an active and replication-independent process, although the mechanisms responsible for it remain elusive. Recently, 5-hydroxymethylcytosine has been suggested as an intermediate in this demethylation. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we quantified DNA methylation and hydroxymethylation in both pronuclei of the mouse zygote during the replication period and we examined their patterns on the pericentric heterochromatin using 3D immuno-FISH. Our results demonstrate that 5-methylcytosine and 5-hydroxymethylcytosine localizations on the pericentric sequences are not complementary; indeed we observe no enrichment of either marks on some regions and an enrichment of both on others. In addition, we show that DNA demethylation continues during DNA replication, and is inhibited by aphidicolin. Finally, we observe notable differences in the kinetics of demethylation and hydroxymethylation; in particular, a peak of 5-hydroxymethylcytosine, unrelated to any change in 5-methylcytosine level, is observed after completion of replication. CONCLUSIONS/SIGNIFICANCE: Together our results support the already proposed hypothesis that 5-hydroxymethylcytosine is not a simple intermediate in an active demethylation process and could play a role of its own during early development.http://europepmc.org/articles/PMC3364968?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Juliette Salvaing Tiphaine Aguirre-Lavin Claire Boulesteix Gaëtan Lehmann Pascale Debey Nathalie Beaujean |
spellingShingle |
Juliette Salvaing Tiphaine Aguirre-Lavin Claire Boulesteix Gaëtan Lehmann Pascale Debey Nathalie Beaujean 5-Methylcytosine and 5-hydroxymethylcytosine spatiotemporal profiles in the mouse zygote. PLoS ONE |
author_facet |
Juliette Salvaing Tiphaine Aguirre-Lavin Claire Boulesteix Gaëtan Lehmann Pascale Debey Nathalie Beaujean |
author_sort |
Juliette Salvaing |
title |
5-Methylcytosine and 5-hydroxymethylcytosine spatiotemporal profiles in the mouse zygote. |
title_short |
5-Methylcytosine and 5-hydroxymethylcytosine spatiotemporal profiles in the mouse zygote. |
title_full |
5-Methylcytosine and 5-hydroxymethylcytosine spatiotemporal profiles in the mouse zygote. |
title_fullStr |
5-Methylcytosine and 5-hydroxymethylcytosine spatiotemporal profiles in the mouse zygote. |
title_full_unstemmed |
5-Methylcytosine and 5-hydroxymethylcytosine spatiotemporal profiles in the mouse zygote. |
title_sort |
5-methylcytosine and 5-hydroxymethylcytosine spatiotemporal profiles in the mouse zygote. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2012-01-01 |
description |
BACKGROUND: In the mouse zygote, DNA methylation patterns are heavily modified, and differ between the maternal and paternal pronucleus. Demethylation of the paternal genome has been described as an active and replication-independent process, although the mechanisms responsible for it remain elusive. Recently, 5-hydroxymethylcytosine has been suggested as an intermediate in this demethylation. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we quantified DNA methylation and hydroxymethylation in both pronuclei of the mouse zygote during the replication period and we examined their patterns on the pericentric heterochromatin using 3D immuno-FISH. Our results demonstrate that 5-methylcytosine and 5-hydroxymethylcytosine localizations on the pericentric sequences are not complementary; indeed we observe no enrichment of either marks on some regions and an enrichment of both on others. In addition, we show that DNA demethylation continues during DNA replication, and is inhibited by aphidicolin. Finally, we observe notable differences in the kinetics of demethylation and hydroxymethylation; in particular, a peak of 5-hydroxymethylcytosine, unrelated to any change in 5-methylcytosine level, is observed after completion of replication. CONCLUSIONS/SIGNIFICANCE: Together our results support the already proposed hypothesis that 5-hydroxymethylcytosine is not a simple intermediate in an active demethylation process and could play a role of its own during early development. |
url |
http://europepmc.org/articles/PMC3364968?pdf=render |
work_keys_str_mv |
AT juliettesalvaing 5methylcytosineand5hydroxymethylcytosinespatiotemporalprofilesinthemousezygote AT tiphaineaguirrelavin 5methylcytosineand5hydroxymethylcytosinespatiotemporalprofilesinthemousezygote AT claireboulesteix 5methylcytosineand5hydroxymethylcytosinespatiotemporalprofilesinthemousezygote AT gaetanlehmann 5methylcytosineand5hydroxymethylcytosinespatiotemporalprofilesinthemousezygote AT pascaledebey 5methylcytosineand5hydroxymethylcytosinespatiotemporalprofilesinthemousezygote AT nathaliebeaujean 5methylcytosineand5hydroxymethylcytosinespatiotemporalprofilesinthemousezygote |
_version_ |
1724990792598028288 |