A comparative study of multiple clinical enterovirus 71 isolates and evaluation of cross protection of inactivated vaccine strain FY-23 K-B in vitro
Abstract Background Enterovirus 71 (EV71) is one of the causative agents of hand, foot and mouth disease, which mostly affects infants and children and leads to severe neurological diseases. Vaccination offers the best option for disease control. We have screened the virus strain FY-23 K-B, which is...
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doaj-2304b0125b46461b994a46ca10b3a4652020-11-24T21:49:50ZengBMCVirology Journal1743-422X2017-10-011411810.1186/s12985-017-0872-8A comparative study of multiple clinical enterovirus 71 isolates and evaluation of cross protection of inactivated vaccine strain FY-23 K-B in vitroTing Yang0Hua Li1Lei Yue2Xia Song3Tianhong Xie4Shaohui Ma5Huaqing Meng6Ye Zhang7Xin He8Runxiang Long9Rong Yang10Fangyu Luo11Zhongping Xie12Qihan Li13Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical CollegeInstitute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical CollegeInstitute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical CollegeInstitute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical CollegeInstitute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical CollegeInstitute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical CollegeInstitute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical CollegeInstitute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical CollegeInstitute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical CollegeInstitute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical CollegeInstitute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical CollegeInstitute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical CollegeInstitute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical CollegeInstitute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical CollegeAbstract Background Enterovirus 71 (EV71) is one of the causative agents of hand, foot and mouth disease, which mostly affects infants and children and leads to severe neurological diseases. Vaccination offers the best option for disease control. We have screened the virus strain FY-23 K-B, which is used as an inactivated vaccine strain. An important issue in the development of vaccines is whether they provide cross protection against all other strains. Methods We collected and identified 19 clinical EV71 isolates from mainland China, which all belong to the C4 genotype. We established growth curves of the strains in Vero cells, performed genetic analysis, and evaluated the cross protection efficacy through neutralizing assays using antisera from a rabbit, monkey and adult human immunized with the FY-23 K-B vaccine strain. Results The antisera showed broad cross protection among the C4 subgroup strains and homotype strain. Neutralizing indexes (NIs) among the isolates and homotype strain of antisera varied between 56.2–1995.3 for rabbit, 17.8–42,169.7 for monkey and 31.6–17,782.8 for human, whereas NIs against Coxsackievirus A16 or other enteroviruses were below 10. Conclusions These results suggested that FY-23 K-B used as an antigen could elicit broad spectrum neutralizing antibodies with cross protective efficacy among C4 genotype strains.http://link.springer.com/article/10.1186/s12985-017-0872-8HandFoot and mouth diseaseEnterovirus 71Cross protectionGenetic conditions |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ting Yang Hua Li Lei Yue Xia Song Tianhong Xie Shaohui Ma Huaqing Meng Ye Zhang Xin He Runxiang Long Rong Yang Fangyu Luo Zhongping Xie Qihan Li |
spellingShingle |
Ting Yang Hua Li Lei Yue Xia Song Tianhong Xie Shaohui Ma Huaqing Meng Ye Zhang Xin He Runxiang Long Rong Yang Fangyu Luo Zhongping Xie Qihan Li A comparative study of multiple clinical enterovirus 71 isolates and evaluation of cross protection of inactivated vaccine strain FY-23 K-B in vitro Virology Journal Hand Foot and mouth disease Enterovirus 71 Cross protection Genetic conditions |
author_facet |
Ting Yang Hua Li Lei Yue Xia Song Tianhong Xie Shaohui Ma Huaqing Meng Ye Zhang Xin He Runxiang Long Rong Yang Fangyu Luo Zhongping Xie Qihan Li |
author_sort |
Ting Yang |
title |
A comparative study of multiple clinical enterovirus 71 isolates and evaluation of cross protection of inactivated vaccine strain FY-23 K-B in vitro |
title_short |
A comparative study of multiple clinical enterovirus 71 isolates and evaluation of cross protection of inactivated vaccine strain FY-23 K-B in vitro |
title_full |
A comparative study of multiple clinical enterovirus 71 isolates and evaluation of cross protection of inactivated vaccine strain FY-23 K-B in vitro |
title_fullStr |
A comparative study of multiple clinical enterovirus 71 isolates and evaluation of cross protection of inactivated vaccine strain FY-23 K-B in vitro |
title_full_unstemmed |
A comparative study of multiple clinical enterovirus 71 isolates and evaluation of cross protection of inactivated vaccine strain FY-23 K-B in vitro |
title_sort |
comparative study of multiple clinical enterovirus 71 isolates and evaluation of cross protection of inactivated vaccine strain fy-23 k-b in vitro |
publisher |
BMC |
series |
Virology Journal |
issn |
1743-422X |
publishDate |
2017-10-01 |
description |
Abstract Background Enterovirus 71 (EV71) is one of the causative agents of hand, foot and mouth disease, which mostly affects infants and children and leads to severe neurological diseases. Vaccination offers the best option for disease control. We have screened the virus strain FY-23 K-B, which is used as an inactivated vaccine strain. An important issue in the development of vaccines is whether they provide cross protection against all other strains. Methods We collected and identified 19 clinical EV71 isolates from mainland China, which all belong to the C4 genotype. We established growth curves of the strains in Vero cells, performed genetic analysis, and evaluated the cross protection efficacy through neutralizing assays using antisera from a rabbit, monkey and adult human immunized with the FY-23 K-B vaccine strain. Results The antisera showed broad cross protection among the C4 subgroup strains and homotype strain. Neutralizing indexes (NIs) among the isolates and homotype strain of antisera varied between 56.2–1995.3 for rabbit, 17.8–42,169.7 for monkey and 31.6–17,782.8 for human, whereas NIs against Coxsackievirus A16 or other enteroviruses were below 10. Conclusions These results suggested that FY-23 K-B used as an antigen could elicit broad spectrum neutralizing antibodies with cross protective efficacy among C4 genotype strains. |
topic |
Hand Foot and mouth disease Enterovirus 71 Cross protection Genetic conditions |
url |
http://link.springer.com/article/10.1186/s12985-017-0872-8 |
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