Toxicokinetics of Hydrolyzed Fumonisin B₁ After Single Oral or Intravenous Bolus to Broiler Chickens Fed a Control or a Fumonisins-Contaminated Diet

• Main Authors: Gunther Antonissen, Siegrid De Baere, Barbara Novak, Dian Schatzmayr, Danica den Hollander, Mathias Devreese, Siska Croubels
• Format: Article
• Language: English
• Published: MDPI AG 2020-06-01
• Series: Toxins
• Subjects: mycotoxins; toxicokinetics; fumonisins metabolites; feeding trial; broiler chicken
• Online Access: https://www.mdpi.com/2072-6651/12/6/413

The toxicokinetics (TK) of hydrolyzed fumonisin B₁ (HFB₁) were evaluated in 16 broiler chickens after being fed either a control or a fumonisins-contaminated diet (10.8 mg fumonisin B₁, 3.3 mg B₂ and 1.5 mg B₃/kg feed) for two weeks, followed by a single oral (PO) or intravenous (IV) dose of 1.25 mg/kg bodyweight (BW) of HFB₁. Fumonisin B₁ (FB₁), its partially hydrolyzed metabolites pHFB_1a and pHFB_1b, and fully hydrolyzed metabolite HFB₁, were determined in chicken plasma using a validated ultra-performance liquid chromatography–tandem mass spectrometry method. None of the broiler chicken showed clinical symptoms of fumonisins (FBs) or HFB₁ toxicity during the trial, nor was an aberration in body weight observed between the animals fed the FBs-contaminated diet and those fed the control diet. HFB₁ was shown to follow a two-compartmental pharmacokinetic model with first order elimination in broiler chickens after IV administration. Toxicokinetic parameters of HFB₁ demonstrated a total body clearance of 16.39 L/kg·h and an intercompartmental flow of 8.34 L/kg·h. Low levels of FB₁ and traces of pHFB_1b were found in plasma of chickens fed the FBs-contaminated diet. Due to plasma concentrations being under the limit of quantification (LOQ) after oral administration of HFB₁, no toxicokinetic modelling could be performed in broiler chickens after oral administration of HFB₁. Moreover, no phase II metabolites, nor N-acyl-metabolites of HFB₁ could be detected in this study.