Regeneration of hyaline-like cartilage in situ with SOX9 stimulation of bone marrow-derived mesenchymal stem cells.
Microfracture, a common procedure for treatment of cartilage injury, induces fibrocartilage repair by recruiting bone marrow derived mesenchymal stem cells (MSC) to the site of cartilage injury. However, fibrocartilage is inferior biomechanically to hyaline cartilage. SRY-type high-mobility group bo...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2017-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC5493350?pdf=render |
id |
doaj-23086210aa0f4eeb9a9ff66dfc1caa88 |
---|---|
record_format |
Article |
spelling |
doaj-23086210aa0f4eeb9a9ff66dfc1caa882020-11-25T00:27:02ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01126e018013810.1371/journal.pone.0180138Regeneration of hyaline-like cartilage in situ with SOX9 stimulation of bone marrow-derived mesenchymal stem cells.Xiaowei ZhangShili WuTy NaccaratoManan Prakash-DamaniYuan ChouCong-Qiu ChuYong ZhuMicrofracture, a common procedure for treatment of cartilage injury, induces fibrocartilage repair by recruiting bone marrow derived mesenchymal stem cells (MSC) to the site of cartilage injury. However, fibrocartilage is inferior biomechanically to hyaline cartilage. SRY-type high-mobility group box-9 (SOX9) is a master regulator of chondrogenesis by promoting proliferation and differentiation of MSC into chondrocytes. In this study we aimed to test the therapeutic potential of cell penetrating recombinant SOX9 protein in regeneration of hyaline cartilage in situ at the site of cartilage injury. We generated a recombinant SOX9 protein which was fused with super positively charged green fluorescence protein (GFP) (scSOX9) to facilitate cell penetration. scSOX9 was able to induce chondrogenesis of bone marrow derived MSC in vitro. In a rabbit cartilage injury model, scSOX9 in combination with microfracture significantly improved quality of repaired cartilage as shown by macroscopic appearance. Histological analysis revealed that the reparative tissue induced by microfracture with scSOX9 had features of hyaline cartilage; and collagen type II to type I ratio was similar to that in normal cartilage. This short term in vivo study demonstrated that when administered at the site of microfracture, scSOX9 was able to induce reparative tissue with features of hyaline cartilage.http://europepmc.org/articles/PMC5493350?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiaowei Zhang Shili Wu Ty Naccarato Manan Prakash-Damani Yuan Chou Cong-Qiu Chu Yong Zhu |
spellingShingle |
Xiaowei Zhang Shili Wu Ty Naccarato Manan Prakash-Damani Yuan Chou Cong-Qiu Chu Yong Zhu Regeneration of hyaline-like cartilage in situ with SOX9 stimulation of bone marrow-derived mesenchymal stem cells. PLoS ONE |
author_facet |
Xiaowei Zhang Shili Wu Ty Naccarato Manan Prakash-Damani Yuan Chou Cong-Qiu Chu Yong Zhu |
author_sort |
Xiaowei Zhang |
title |
Regeneration of hyaline-like cartilage in situ with SOX9 stimulation of bone marrow-derived mesenchymal stem cells. |
title_short |
Regeneration of hyaline-like cartilage in situ with SOX9 stimulation of bone marrow-derived mesenchymal stem cells. |
title_full |
Regeneration of hyaline-like cartilage in situ with SOX9 stimulation of bone marrow-derived mesenchymal stem cells. |
title_fullStr |
Regeneration of hyaline-like cartilage in situ with SOX9 stimulation of bone marrow-derived mesenchymal stem cells. |
title_full_unstemmed |
Regeneration of hyaline-like cartilage in situ with SOX9 stimulation of bone marrow-derived mesenchymal stem cells. |
title_sort |
regeneration of hyaline-like cartilage in situ with sox9 stimulation of bone marrow-derived mesenchymal stem cells. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2017-01-01 |
description |
Microfracture, a common procedure for treatment of cartilage injury, induces fibrocartilage repair by recruiting bone marrow derived mesenchymal stem cells (MSC) to the site of cartilage injury. However, fibrocartilage is inferior biomechanically to hyaline cartilage. SRY-type high-mobility group box-9 (SOX9) is a master regulator of chondrogenesis by promoting proliferation and differentiation of MSC into chondrocytes. In this study we aimed to test the therapeutic potential of cell penetrating recombinant SOX9 protein in regeneration of hyaline cartilage in situ at the site of cartilage injury. We generated a recombinant SOX9 protein which was fused with super positively charged green fluorescence protein (GFP) (scSOX9) to facilitate cell penetration. scSOX9 was able to induce chondrogenesis of bone marrow derived MSC in vitro. In a rabbit cartilage injury model, scSOX9 in combination with microfracture significantly improved quality of repaired cartilage as shown by macroscopic appearance. Histological analysis revealed that the reparative tissue induced by microfracture with scSOX9 had features of hyaline cartilage; and collagen type II to type I ratio was similar to that in normal cartilage. This short term in vivo study demonstrated that when administered at the site of microfracture, scSOX9 was able to induce reparative tissue with features of hyaline cartilage. |
url |
http://europepmc.org/articles/PMC5493350?pdf=render |
work_keys_str_mv |
AT xiaoweizhang regenerationofhyalinelikecartilageinsituwithsox9stimulationofbonemarrowderivedmesenchymalstemcells AT shiliwu regenerationofhyalinelikecartilageinsituwithsox9stimulationofbonemarrowderivedmesenchymalstemcells AT tynaccarato regenerationofhyalinelikecartilageinsituwithsox9stimulationofbonemarrowderivedmesenchymalstemcells AT mananprakashdamani regenerationofhyalinelikecartilageinsituwithsox9stimulationofbonemarrowderivedmesenchymalstemcells AT yuanchou regenerationofhyalinelikecartilageinsituwithsox9stimulationofbonemarrowderivedmesenchymalstemcells AT congqiuchu regenerationofhyalinelikecartilageinsituwithsox9stimulationofbonemarrowderivedmesenchymalstemcells AT yongzhu regenerationofhyalinelikecartilageinsituwithsox9stimulationofbonemarrowderivedmesenchymalstemcells |
_version_ |
1725341381145133056 |