Saliva microRNA Biomarkers of Cumulative Concussion
Recurrent concussions increase risk for persistent post-concussion symptoms, and may lead to chronic neurocognitive deficits. Little is known about the molecular pathways that contribute to persistent concussion symptoms. We hypothesized that salivary measurement of microribonucleic acids (miRNAs),...
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2020-10-01
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doaj-231a0b76957f4f4bbfccf203c80b1a7d2020-11-25T03:06:10ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-10-01217758775810.3390/ijms21207758Saliva microRNA Biomarkers of Cumulative ConcussionSteven D. Hicks0Robert P. Olympia1Cayce Onks2Raymond Y. Kim3Kevin J. Zhen4Gregory Fedorchak5Samantha DeVita6Aakanksha Rangnekar7Matthew Heller8Hallie Zwibel9Chuck Monteith10Zofia Gagnon11Callan D. McLoughlin12Jason Randall13Miguel Madeira14Thomas R. Campbell15Elise Fengler16Michael N. Dretsch17Christopher Neville18Frank A. Middleton19Department of Pediatrics, Penn State College of Medicine, Hershey, PA 17033, USADepartment of Emergency Medicine, Penn State College of Medicine, Hershey, PA 17033, USADepartment of Family Medicine, Penn State College of Medicine, Hershey, PA 17033, USADepartment of Orthopedics and Rehabilitation, Penn State College of Medicine, Hershey, PA 17033, USADepartment of Pediatrics, Penn State College of Medicine, Hershey, PA 17033, USAQuadrant Biosciences, Institute for Human Performance, Syracuse, NY 13210, USAQuadrant Biosciences, Institute for Human Performance, Syracuse, NY 13210, USAQuadrant Biosciences, Institute for Human Performance, Syracuse, NY 13210, USADepartment of Family Medicine, New York Institute of Technology College of Osteopathic Medicine, Old Westbury, NY 11568, USADepartment of Family Medicine, New York Institute of Technology College of Osteopathic Medicine, Old Westbury, NY 11568, USADepartment of Athletic Training, Colgate University, Hamilton, NY 13346, USASchool of Science, Marist College, Poughkeepsie, NY 12601, USASchool of Science, Marist College, Poughkeepsie, NY 12601, USASchool of Science, Marist College, Poughkeepsie, NY 12601, USASchool of Science, Marist College, Poughkeepsie, NY 12601, USASchool of Rehabilitation Sciences, Old Dominion University, Norfolk, VA 23529, USADepartment of Exercise Science, Syracuse University, Syracuse, NY 13210, USAUS Army Medical Research Directorate-West, Walter Reed Army Institute of Research, Joint Base Lewis-McChord, WA 98433, USADepartment of PT Education, Orthopedics, and Neuroscience, SUNY Upstate Medical University, Syracuse, NY 13210, USADepartment of Neuroscience and Physiology, SUNY Upstate Medical University, Syracuse, NY 13210, USARecurrent concussions increase risk for persistent post-concussion symptoms, and may lead to chronic neurocognitive deficits. Little is known about the molecular pathways that contribute to persistent concussion symptoms. We hypothesized that salivary measurement of microribonucleic acids (miRNAs), a class of epitranscriptional molecules implicated in concussion pathophysiology, would provide insights about the molecular cascade resulting from recurrent concussions. This hypothesis was tested in a case-control study involving 13 former professional football athletes with a history of recurrent concussion, and 18 age/sex-matched peers. Molecules of interest were further validated in a cross-sectional study of 310 younger individuals with a history of no concussion (<i>n</i> = 230), a single concussion (<i>n </i>= 56), or recurrent concussions (<i>n</i> = 24). There was no difference in neurocognitive performance between the former professional athletes and their peers, or among younger individuals with varying concussion exposures. However, younger individuals without prior concussion outperformed peers with prior concussion on three balance assessments. Twenty salivary miRNAs differed (adj. <i>p</i> < 0.05) between former professional athletes and their peers. Two of these (miR-28-3p and miR-339-3p) demonstrated relationships (<i>p</i> < 0.05) with the number of prior concussions reported by younger individuals. miR-28-3p and miR-339-5p may play a role in the pathophysiologic mechanism involved in cumulative concussion effects.https://www.mdpi.com/1422-0067/21/20/7758salivamicroRNAsports-related concussionmild traumatic brain injurybiomarker |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Steven D. Hicks Robert P. Olympia Cayce Onks Raymond Y. Kim Kevin J. Zhen Gregory Fedorchak Samantha DeVita Aakanksha Rangnekar Matthew Heller Hallie Zwibel Chuck Monteith Zofia Gagnon Callan D. McLoughlin Jason Randall Miguel Madeira Thomas R. Campbell Elise Fengler Michael N. Dretsch Christopher Neville Frank A. Middleton |
spellingShingle |
Steven D. Hicks Robert P. Olympia Cayce Onks Raymond Y. Kim Kevin J. Zhen Gregory Fedorchak Samantha DeVita Aakanksha Rangnekar Matthew Heller Hallie Zwibel Chuck Monteith Zofia Gagnon Callan D. McLoughlin Jason Randall Miguel Madeira Thomas R. Campbell Elise Fengler Michael N. Dretsch Christopher Neville Frank A. Middleton Saliva microRNA Biomarkers of Cumulative Concussion International Journal of Molecular Sciences saliva microRNA sports-related concussion mild traumatic brain injury biomarker |
author_facet |
Steven D. Hicks Robert P. Olympia Cayce Onks Raymond Y. Kim Kevin J. Zhen Gregory Fedorchak Samantha DeVita Aakanksha Rangnekar Matthew Heller Hallie Zwibel Chuck Monteith Zofia Gagnon Callan D. McLoughlin Jason Randall Miguel Madeira Thomas R. Campbell Elise Fengler Michael N. Dretsch Christopher Neville Frank A. Middleton |
author_sort |
Steven D. Hicks |
title |
Saliva microRNA Biomarkers of Cumulative Concussion |
title_short |
Saliva microRNA Biomarkers of Cumulative Concussion |
title_full |
Saliva microRNA Biomarkers of Cumulative Concussion |
title_fullStr |
Saliva microRNA Biomarkers of Cumulative Concussion |
title_full_unstemmed |
Saliva microRNA Biomarkers of Cumulative Concussion |
title_sort |
saliva microrna biomarkers of cumulative concussion |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2020-10-01 |
description |
Recurrent concussions increase risk for persistent post-concussion symptoms, and may lead to chronic neurocognitive deficits. Little is known about the molecular pathways that contribute to persistent concussion symptoms. We hypothesized that salivary measurement of microribonucleic acids (miRNAs), a class of epitranscriptional molecules implicated in concussion pathophysiology, would provide insights about the molecular cascade resulting from recurrent concussions. This hypothesis was tested in a case-control study involving 13 former professional football athletes with a history of recurrent concussion, and 18 age/sex-matched peers. Molecules of interest were further validated in a cross-sectional study of 310 younger individuals with a history of no concussion (<i>n</i> = 230), a single concussion (<i>n </i>= 56), or recurrent concussions (<i>n</i> = 24). There was no difference in neurocognitive performance between the former professional athletes and their peers, or among younger individuals with varying concussion exposures. However, younger individuals without prior concussion outperformed peers with prior concussion on three balance assessments. Twenty salivary miRNAs differed (adj. <i>p</i> < 0.05) between former professional athletes and their peers. Two of these (miR-28-3p and miR-339-3p) demonstrated relationships (<i>p</i> < 0.05) with the number of prior concussions reported by younger individuals. miR-28-3p and miR-339-5p may play a role in the pathophysiologic mechanism involved in cumulative concussion effects. |
topic |
saliva microRNA sports-related concussion mild traumatic brain injury biomarker |
url |
https://www.mdpi.com/1422-0067/21/20/7758 |
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