Determination of collagen content within picrosirius red stained paraffin-embedded tissue sections using fluorescence microscopy
Picrosirius red (PSR) staining is a commonly used histological technique to visualize collagen in paraffin-embedded tissue sections. PSR stained collagen appears red in light microscopy. However it is largely unknown that PSR stained collagen also shows a red fluorescence, whereas live cells have a...
Main Authors: | Benjamin Vogel, Hanna Siebert, Ulrich Hofmann, Stefan Frantz |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2015-01-01
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Series: | MethodsX |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016115000102 |
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